Protein Alignment Sec16 and SEC16

DIOPT Version :9

• Sequence 1: NP_001285158.1
• Gene: Sec16 / 32209
• FlyBaseID: FBgn0052654
• Length: 2326
• Species: Drosophila melanogaster
• Sequence 2: NP_015240.1
• Gene: SEC16 / 856020
• SGDID: S000006006
• Length: 2195
• Species: Saccharomyces cerevisiae
• Alignment Length: 2223
• Identity: 418/2223 - (18%)
• Similarity: 703/2223 - (31%)
• Gaps: 785/2223 - (35%)

  Fly   495 HLEPDNQEVLSAPN-----------------------DERAQYLQTS------HLSEQLGEGEAD 530
            |.|..|....||||                       |||:...:||      | .:|:.||:.|
Yeast   328 HNENTNNTQESAPNTDDRDKGYEGNEALKKSESCTAADERSYSEETSEDIFHGH-DKQVVEGQND 391

  Fly   531 -------QDAGLLPPPGLSRLVLG------QPELDSQQQ-----RQVTG---------ATEQPPL 568
                   .::..|...|..:|.|.      :|..|.|.|     .|.:|         :|::...
Yeast   392 FTGKNIENESQKLMGEGNHKLPLSAEADIIEPGKDIQDQAEDLFTQSSGDLGEVLPWESTDKNAD 456

  Fly   569 NVAQAAALHMQERRADGED------TSDGEQQVRNIQTPPRRVVTGVETNAPSLREQREVVLDGE 627
            ..:::...|.....|.|.|      .||||       ....:....::|:...:.||:...|:.:
Yeast   457 VTSKSQEKHEDLFAASGNDEKLPWEVSDGE-------VSSGKTENSMQTSTEKIAEQKFSFLEND 514

  Fly   628 ------------NLEDREAIP----PPTLAELPT----TSVHHNILPDEA----EQLHHNNPPQA 668
                        :.|:.:.:|    ...|...|.    .|.:..|:.:||    ||:|..|....
Yeast   515 DDLLDDDDSFLASSEEEDTVPNTDNTTNLTSKPVEEKKASRYKPIIEEEAGMRQEQVHFTNTTGI 579

  Fly   669 MTPLNAQQPHQQQST-LQQQHQQHSTTQ--QEKKRAAVGRRTTASLDLESDESDEFLQSERERDR 730
            :||   ||.|....| |...:||.|...  ..|.......|:...::.|..:||.:
Yeast   580 VTP---QQFHGLTKTGLGTPNQQVSVPNIVSPKPPVVKDNRSNFKINEEKKKSDAY--------- 632

  Fly   731 ERERRDLMEERHSRGRSHSHYPYEGETEDSVRGAAHHETKSLRETHHKRNHDSARSRRHQDP-KV 794
                               .:|.|..:|.|.:|                         |..| .|
Yeast   633 -------------------DFPLEIISESSKKG-------------------------HAKPVAV 653

  Fly   795 ERERERDRERDRTWRRRSNKYHSGG--EDPDRSYDHSRRYNNSNYEGESDNPEYNHMGDAELDGS 857
            ..:|                :.||.  ...|:....||:.:|:     |:.|....:|..|
Yeast   654 PTQR----------------FGSGNSFSSLDKPIPQSRKGSNN-----SNRPPVIPLGTQE---- 693

  Fly   858 GGRSSKTSRHRRSAAEEDFDDY--------ERERNRSRRSTKP---------------QSSAEKS 899
             .|||:|:    ||..:...:|        ::.:....:|..|               ..||...
Yeast   694 -PRSSRTN----SAISQSPVNYAFPNPYKIQQLQQAPIQSGMPLPNTNIPPPALKVETTVSAPPI 753

  Fly   900 RSSGGRRNYENSGRSARADDGRRRYQDQRNPGAQYPVSATGYVPYGMYEQMSRNPQVYADMYAKF 964
            |:    |...|:...:.|..|.|.       ..||.:: .|..|...|.|.:.|... |:.|
Yeast   754 RA----RGVSNASVGSSASFGARH-------ATQYGLN-NGVPPVSPYGQATINLPT-ANKY--- 802

  Fly   965 YGQMINSMNAAVSAAASKGGVPAGAGVIPGLMPSAVPVSAAQLVAATSGGSVSGSSEAAMLRERE 1029
                     |.||....:...|:   |:..|..||  |:....|....|.:.|.||
Yeast   803 ---------APVSPTVQQKQYPS---VVQNLGASA--VNTPNFVKTHRGHTSSISS--------- 844

  Fly  1030 RYTHAYITQANEFHRHQYKELIYQQQQQQQQQQQ-----------QQQQREDHLNSSFSVAEDNA 1083
                 |....|| |..:|.. .|||..|.....|           |.|.|..:.......|:.:|
Yeast   845 -----YTPNQNE-HASRYAP-NYQQSYQVPYTSQPVGPVAGNSSYQSQTRSSYAVPMMPQAQTSA 902

  Fly  1084 SFYGSHGGGSIYNQYQSYPLSSARSLSNLNGDGRNSRCGPYYAGSECGLDIRAAADAAPSTYGGV 1148
            |.. .|  .:|.......||:..|.|..|                      :||.:..|......
Yeast   903 SIQ-PH--ANIQPPTGILPLAPLRPLDPL----------------------QAATNLQPRASNIT 942

  Fly  1149 AGTAGPVTN-----------------TAVARPPRRRTPLQFSRPHLVA--------SYAMSLLLK 1188
            |..:.|:.|                 ::||.|.:...|::.....|:.        |.|..::..
Yeast   943 AANSLPLANLPLAENILPEIITHRATSSVAPPRQENNPIKIDNEALLRRQFPIFHWSAANKVVYA 1007

  Fly  1189 VKP-----KYAGRGRLRNDVEVAPPRIRDGTSSLLRMYPGPLQGRKLHKDKIISFCKEQIRLGPT 1248
            |.|     :|.....:..:::|.|.......:.:|:.:||||...||.|..:..:.:..|:
Yeast  1008 VPPIPDQSQYMISSSIVQEIKVTPIDQIIKPNDMLKSFPGPLGSAKLKKKDLTKWMETTIK---- 1068

  Fly  1249 KGCTALYATQKKPQGSVTKYRASHAL-MWHLLILLLRQNGYIADTDVGDLLLENQQEYPYDPSEF 1312
                           |:::..:|..: :|.||.:.|  |..:...::..||..:.:...|....|
Yeast  1069 ---------------SISENESSTDMTIWQLLEMKL--NDKVNWKNISKLLYNSDELLMYLSQPF 1116

  Fly  1313 EAENEPDADAEQDAAAPADKTVDSDLDSESAAGVTPAEPLAAGAATSSINGADAANAATPLSEQA 1377
                 |:.|                              :...|....||
Yeast  1117 -----PNGD------------------------------MIPNAYRLDIN--------------- 1131

  Fly  1378 ATDKFRSYVLRGNVEEALQWATDNGLWTHAFFLA--LYEDRYALTDVAQKFLNRAIKA--NDP-- 1436
            ...:..:::..||.:|||:.|.....:..|..:.  :.:||:  ::|.||:|.....|  ||.
Yeast  1132 CQMRVLAFLQTGNHDEALRLALSKRDYAIALLVGSLMGKDRW--SEVIQKYLYEGFTAGPNDQKE 1194

  Fly  1437 ----LQTLYQ---------MKSCHTPACVSQLRDEQWGDWRSHLSILVTNKSRQPEYDRSSVVAL 1488
                |..::|         :||.:|....||...|   :|:|.::.::.|   .||.:...::
Yeast  1195 LAHFLLLIFQVFVGNSKMAIKSFYTNNETSQWASE---NWKSIVAAVLIN---IPENNEDPLL-- 1251

  Fly  1489 GDTLFQRGDIYAAHFCYLVAQEEFGRYDSSATELTT--LTANVPRLILLGS-------------- 1537
                     |......:|:   |||.:      ||.  |||....|.::|:
Yeast  1252 ---------IPPVVLEFLI---EFGIF------LTKKGLTAAASTLFIIGNVPLSNEPVMADSDV 1298

  Fly  1538 --SHYKHFNEFASNEAIIMTEIYEYARSLFDPKF-SIAHFQHYKFLLATRILDYGQHFRCTNYLE 1599
              ....:.|.|   |:|:..|||||..| :|||| ..:.....|...|:.:.:.|.:...|.|.:
Yeast  1299 IFESIGNMNTF---ESILWDEIYEYIFS-YDPKFKGFSSILPQKIYHASLLQEQGLNSLGTKYTD 1359

  Fly  1600 QIARHIELKPES--YDSNFIQRVCGLAERL-------------------------RY---HDPIL 1634
            .::..:...|:.  ...|..:.:..:|.||                         :|   .|...
Yeast  1360 YLSSSVRKLPKKDILTINLTRELSEVASRLSESNTGWLAKPKLSSVWGQLDKSFNKYIGGDDIDA 1424

  Fly  1635 IN------RVSFASPPNATSKDSAAAEEKAWL---RQLRSLAYVQPQQEQLQQLQQNQQVQQEQN 1690
            :|      :|.....|.:::..|.....:.:.   .|:.|.:||     ....|..|.......:
Yeast  1425 LNKKNDKKKVFDGFTPGSSANSSTVDLTQTFTPFQAQVTSQSYV-----DTTALLHNAHNVPSHS 1484

  Fly  1691 DIDQQFAEVNKQFRELNMQYE---------SNSNMENT----LQPQLPPV-----DQQPPQ---Q 1734
            .:..:.:.|:|...|.|:.|.         |...:.||    .:||:..:     ||...:   :
Yeast  1485 VLHSKPSNVSKGLVEANLPYTHRIGDSLQGSPQRIHNTQFAAAEPQMASLRRVRTDQHTNEKALK 1549

  Fly  1735 QQQVLSESYQQPTQQY---------------------YEPPPQAPTESDPYGQ------------ 1766
            .||:|.:.....|.|:                     :..||:..|....|..
Yeast  1550 SQQILEKKSTAYTPQFGQNHSVPMEKSNSNVPSLFADFPAPPKLGTVPSNYVSSPDLVRRESIIS 1614

  Fly  1767 -----------GQPSYYDPNAGQLHYDPNAAQHQYDQQQPQPQPTPSYGQIEPGTEAYQPGQATA 1820
                       |.|:..:.:.|.|.|.|:......|...||...|   |..:.|.:..|......
Yeast  1615 TGSEFLPPPKIGVPTKANSSQGSLMYSPSVEALPIDPVVPQVHET---GYNDFGNKHSQKSMPED 1676

  Fly  1821 DPAAVPSGYGYDYWSGTQQPPYGDEPNENQAALKDEEEEDEE---------QDEQQILQYAKQ-- 1874
            :             |.|..    |..|.:|..|||..:..:|         .|.:.:|.....
Yeast  1677 E-------------SHTSH----DNSNADQNTLKDSADVTDETMDIEGPGFNDVKNLLPMEPNHQ 1724

  Fly  1875 --------QILQQTKQQQLQQEQHTRSNGAASINNRFKSNALKQERSSGSLRKRQEASRTATIAA 1931
                    |.:....|..||.....|...|:.:.|...|  ::.||||      :|.....:.:|
Yeast  1725 PTSTVNPIQTISDDIQPILQTNVEVRGTDASKMENSLPS--IENERSS------EEQPENISKSA 1781

  Fly  1932 AAAAIAQITPATVAAAATPPAASAKAFNLNDVSSFSSTNKRHQATSSSNNRSSSNQAA---AAAA 1993
            ::|.:    |:|                    ...|..|:  ..|...|:.|.:.|:.   |.:.
Yeast  1782 SSAYL----PST--------------------GGLSLENR--PLTQDENSISETVQSTYLPAGSI 1820

  Fly  1994 ATKAAAIATVPAAPNQLLSCRKSNSQNSPYLATPKTLTTPTRFIAAKAATVTATPTAP------- 2051
            :.:|..|:.|...|..:.:  |::.....::|.||..:|       .|..:..:|..|
Yeast  1821 SMEAKPISQVQDVPRNVNN--KASKLVEQHMAPPKPKST-------DATKMNYSPYVPQSTAASA 1876

  Fly  2052 ----ATSTTTSTTIGARGSSTKRVYFNLPSTIAESEQEFEHELDYDEEMEEENSMALAQAKPNVH 2112
                :|...||..|.||   |.:.:.:.||      |.|......:|....|.|.:.:||:.|.:
Yeast  1877 DGDESTILKTSPAIYAR---THQAHASNPS------QYFPLVNQANETASFELSESTSQAQSNGN 1932

  Fly  2113 LA--------RKPHIVFNGTYPIDMPLTQGQSQLKSNSYDP-EANADRGDLQRYRNYMLHDYVID 2168
            :|        :|..:|...|:   .|..:..|..:..::.| |::||:           ::.||:
Yeast  1933 VASENRFSPIKKAEVVEKDTF---QPTIRKASTNQYRAFKPLESDADK-----------YNDVIE 1983

  Fly  2169 EDAEGEEQAEEEGE-----------EDEYGDGDEDLFPYADDDES--------FDDGD------- 2207
            ::::.:..:.:|.:           :.|....::|:     ||:|        .|.||
Yeast  1984 DESDDDNMSTDEAKNRKEEKKNVNMKKETKPSNKDI-----DDKSNGWFGWLKKDTGDKKVYKAK 2043

  Fly  2208 -------YYTHQL---LNKYGIIPSGEKQRVERTPASVEAATSAAQPQHVWSMKELIANPAI--- 2259
                   ||..:|   :||..  ...|||::      :|  :||..|..:...|:  ..|..
Yeast  2044 LGHKNTLYYDEKLKRWVNKDA--TEEEKQKI------IE--SSAPPPPPIVKRKD--GGPKTKPR 2096

  Fly  2260 --PLNYKKMCSEVEQSLSRFENYLDSKKEQQVPP-----GAAVRTFDIDAPTRRGHRSSTSTN-- 2315
              |:|                |.|.......|.|     |..:......:||.....:|.|.:
Yeast  2097 SGPIN----------------NSLPPVHATSVIPNNPITGEPLPIKTSPSPTGPNPNNSPSPSSP 2145

  Fly  2316 ----AGLDLRSKK 2324
                :|::|.|||
Yeast  2146 ISRISGVNLTSKK 2158

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sec16	NP_001285158.1	ACE1-Sec16-like	1371..1631	CDD:187750	66/327 (20%)
SEC16	NP_015240.1	Sec16_N	291..567	CDD:315590	48/246 (20%)
		PHA03247	<680..980	CDD:223021	76/384 (20%)
		Sec16	994..1093	CDD:403977	23/119 (19%)
		Sec16_C	1136..1420	CDD:403976	66/315 (21%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157346138
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001693
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR13402
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1306
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	5.060