DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Pkcdelta and Pkn2

DIOPT Version :9

Sequence 1:	NP_572797.3	Gene:	Pkcdelta / 32191	FlyBaseID:	FBgn0259680	Length:	1894	Species:	Drosophila melanogaster
Sequence 2:	NP_848769.2	Gene:	Pkn2 / 109333	MGIID:	109211	Length:	983	Species:	Mus musculus

Alignment Length:	969	Identity:	271/969 - (27%)
Similarity:	416/969 - (42%)	Gaps:	205/969 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly  1017 TATPTTPNTPTTATGKS-------KLNETAGPKINSGQPAKSPEQQQATAAAVETKINKTT---- 1070
            |..|.||:||.:.:..|       .|.:....::...|.|::..|..:..::.:.|::.|.
Mouse   116 TDCPRTPDTPNSDSRSSTSNNRLMALQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLL 180

  Fly  1071 -DKEQQQQQQQQQQQQAQQQQQQLIDTAATLVPQTETKTK------LTEAAEPTGKSKVQSPNTT 1128
             |.:.:.:..:.|..||.|..:...|.|..::...|.:.:      ..|.|...|...|.
Mouse   181 QDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELRMEELRHHFKIEFAVAEGAKNVM----- 240

  Fly  1129 KPKSAGKTNVGKKKAATPAAAAAAAAAAAPAAATATEAVTALSES----NVTPINADQFITLAPT 1189
              |..|...|..:||                   .:||....:||    ::...:.:|.:...|.
Mouse   241 --KLLGSGKVTDRKA-------------------LSEAQARFNESSQKLDLLKYSLEQRLNELPR 284

  Fly  1190 KAAKTSPNKQQALQLQQQHEEQQRQQ----QQQATNASSPAATGDATTTPTTITIGVAAV--NVA 1248
            ...|:|...:: |.|........||.    |.|.:..|.|||   .|.|.....:|...:  ||.
Mouse   285 NHPKSSVVIEE-LSLVASPTLSPRQSMLSTQNQYSTLSKPAA---LTGTLEVRLMGCQDILENVP 345

  Fly  1249 AVAVVASAAAAAATDAAADATTSLSSNNSCGEGVGNNLSKFATVSNLAQCLRDVDA--ELDDYI- 1310
            ..: .|::.|......:.:.::.:|..:....|...||.|...:||      ||.|  :||:.:
Mouse   346 GRS-KATSVALPGWSPSDNRSSFMSRTSKSKSGSSRNLLKTDDLSN------DVCAVLKLDNTVV 403

  Fly  1311 ------PSSAENSQEDDDDDDDGSGSSS-----DDYSSVDGCA---------------------- 1342
                  |.|.::..:....:.|.|....     .|:.|:  ||
Mouse   404 GQTSWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSL--CAVKFLRLEDFLDNQRHGMCLYLE 466

  Fly  1343 ------------NLSASMKKKLRKEKK---KQKAKAAAAAEAKRFDPHKKIKIDTTNKCYVKEE- 1391
                        |.....:.||:::||   ||:.|....|      |...|.|.|..: .|:..
Mouse   467 PQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRA------PQMNINIATWGR-LVRRAI 524

  Fly  1392 ---------APRYPLVATPRPLWKREKIVYSDENTDD-ESGSEEGSGGSLDEDSDDE--SGGEEC 1444
                     :|:.|:.||         :...|....| ...:.:.:...||.|.:.|  ......
Mouse   525 PTVNHSGTFSPQTPVPAT---------VPVVDARIPDLAPPASDSTVTKLDFDLEPEPPPAPPRA 580

  Fly  1445 SSTSSSASSEDLDAVAMVTKAGSSNATTVLDSSNS---SGSNAGTLSVPSAGSGSGGGASTSSSP 1506
            ||...:..|.:| .|..:...||.....:.:..|:   ...:...||:|.:|....|...
Mouse   581 SSLGETDESSEL-RVLDIPGQGSETVFNIENDRNNLRPKSKSEYELSIPDSGRSCWGVGE----- 639

  Fly  1507 SIIRMSTCSNDSGFEGGTAPSSPKKMLETSYTYSQFQKSGRFTAPATVIPRFKNYSVDDFHFLAV 1571
                                      |:......:||                 :|:.||...||
Mouse   640 --------------------------LDDKRAQQRFQ-----------------FSLQDFRCCAV 661

  Fly  1572 LGKGSFGKVLLAELRDTTYYYAIKCLKKDVVLEDDDVDSTLIERKVLAL--GTKHPYLCHLFCTF 1634
            ||:|.||||||||.:.|...:|||.|||..::..|:|||.:.|:::...  ..:||:|.:||..|
Mouse   662 LGRGHFGKVLLAEYKHTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACF 726

  Fly  1635 QTESHLFFVMEYLNGGDLMFHIQESGRFSEERARFYGAEIISGLKFLHKKGIIYRDLKLDNVLLD 1699
            ||:.|:.|||||..|||||.|| .:..|||.||.||.|.::.||::||:..|:||||||||:|||
Mouse   727 QTKEHVCFVMEYAAGGDLMMHI-HTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLD 790

  Fly  1700 YEGHVRIADFGMCKLQI-YLDKTADSFCGTPDYMAPEIIKGEKYNQNVDWWSFGVLLYEMLIGQS 1763
            .||.|:|||||:||..: |.|:|: :|||||:::|||::....|.:.||||..|||:||||:|:|
Mouse   791 TEGFVKIADFGLCKEGMGYGDRTS-TFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGES 854

  Fly  1764 PFSGCDEDELFWSICNEIPWFPVYISAEATGILKGLLEKDYTKRIGSQYSPAGDIADHIFFRPID 1828
            ||.|.||:|:|.||.|:...:|.::|.||..|::.||.::..:|:|:....|.|:..|.|||..|
Mouse   855 PFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGEKDAEDVKKHPFFRLTD 919

  Fly  1829 WGLLEKRQIEPPFKPQVKHPLDTQYFDRVFTRERVRLT-PIDKEILASMDQKQFHGFTY 1886
            |..|..::::|||.|.::...|...||..||.|...|| |.:..||...:|:.||.|.|
Mouse   920 WSALMDKKVKPPFVPTIRGREDVSNFDDEFTSEAPILTPPREPRILLEEEQEMFHDFDY 978

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pkcdelta	NP_572797.3	S_TKc	1566..1824	CDD:214567	128/260 (49%)
Pkcdelta	NP_572797.3	STKc_nPKC_theta_like	1570..1889	CDD:270744	153/321 (48%)
Pkn2	NP_848769.2	HR1_PKN_1	42..107	CDD:212012
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	107..135		7/18 (39%)
		HR1_PKN2_2	128..201	CDD:212021	12/72 (17%)
		HR1_PKN2_3	209..282	CDD:212025	15/98 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	351..382		3/30 (10%)
		Necessary to rescue apical junction formation	381..462		18/88 (20%)
		C2_PKN-like	389..472	CDD:176069	15/90 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	553..588		6/34 (18%)
		STKc_PKN	656..981	CDD:270741	154/325 (47%)
		S_TKc	661..915	CDD:214567	126/255 (49%)
		Necessary for the catalytic activity. /evidence=ECO:0000250	916..976		22/59 (37%)
		Negatively regulates the responsiveness of the catalytic activity by cardiolipin and is required for optimal activation by the GTP-bound RhoA. /evidence=ECO:0000250	977..983		1/2 (50%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167839290
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R891
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.870