DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment cac and Scn4a

DIOPT Version :9

Sequence 1:	NP_001356945.1	Gene:	cac / 32158	FlyBaseID:	FBgn0263111	Length:	2110	Species:	Drosophila melanogaster
Sequence 2:	NP_037310.2	Gene:	Scn4a / 25722	RGDID:	3636	Length:	1840	Species:	Rattus norvegicus

Alignment Length:	1847	Identity:	462/1847 - (25%)
Similarity:	819/1847 - (44%)	Gaps:	385/1847 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFL 81
            :|::|:..:.:|:....::....|...:::||:.|||.:.: .:.|...|.|        |..|.
  Rat   110 ALYLLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTM-SNPPSWSKHV--------EYTFT 165

  Fly    82 CIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPL 146
            .|:..|:.:|:||.|..:...::||:.||.:||.|:...::|::..:|   ::..||..||||.|
  Rat   166 GIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLG---NISALRTFRVLRAL 227

  Fly   147 KLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDP------ 205
            |.::.||.|:.::.::|:::..|..:.:|.:|.:.:||::||:.:.|.|.:.|.....|
  Rat   228 KTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPMNDTNT 292

  Fly   206 ----------------------NKLVKEGES---------ETPCNTDN---ILEKATGSFVCNNT 236
                                  |......||         |...|.:.   .||.:..:.:|.|:
  Rat   293 TWYGNDTWYSNDTWYGNDTWYINDTWNSQESWAGNSTFDWEAYINDEGNFYFLEGSNDALLCGNS 357

  Fly   237 TSM--------CLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYF 293
            :..        |::....||.|.||:|...:|.|.:|:.:|.:.|..:...|..|.|..: .|:|
  Rat   358 SDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTY-MIFF 421

  Fly   294 VPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILA 358
            |.:|.:|||:::||:|.|::..:|::                                 .|..||
  Rat   422 VVIIFLGSFYLINLILAVVAMAYAEQ---------------------------------NEATLA 453

  Fly   359 EERTTEEEKMHIMEARRRNAAKRKKLKSLGKSKSTDTEEEEAEEDYGDD--GYLKT---RSKPQG 418
            |::..|||...::|..:::..:.:|.|:....:|  .||.:.:..:..|  |.|..   :..|:.
  Rat   454 EDQEKEEEFQQMLEKYKKHQEEVEKAKAAQALES--GEEADGDPTHNKDCNGSLDASGEKGPPRP 516

  Fly   419 SCTG---------------------FWRAEKRFRFW--------IRHTVKTQWFYWFVI------ 448
            ||:.                     :::...:...|        .:|.:     |..|:
  Rat   517 SCSADSAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHII-----YLIVMDPFVDL 576

  Fly   449 ---VLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDC 510
               :.:.|||:.:|:|||.........|.....:|.|:|.:||.:|:.|:.|..||:..:|.||.
  Rat   577 GITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDS 641

  Fly   511 VVISGSIFEVIWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLF 575
            .:::.|:.|:..:.|:    ||||||:.||||:||:.|.|.:|..|:..:.||:.::.:|..:|.
  Rat   642 FIVTLSLVELGLANVQ----GLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLA 702

  Fly   576 LFILIFALLGMQLFGGQF-----------NLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQ 629
            :.:.|||::||||||..:           ||    |..:.|.|..:.|.||:||.|| |.|.|:.
  Rat   703 IIVFIFAVVGMQLFGKSYKECVCKIASDCNL----PRWHMNDFFHSFLIVFRILCGE-WIETMWD 762

  Fly   630 GIISQGGAQKGMIYSIYFIVLVLFGNYTLLNVFLAI-----AVDNLANAQE-------------- 675
            .:...|.|   |..:::.:|:|: ||..:||:|||:     :.|:||.:.|
  Rat   763 CMEVAGQA---MCLTVFLMVMVI-GNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRI 823

  Fly   676 --------------------------LTAAE--------EEQVEEDKEKQLQELEKEME------ 700
                                      |:..|        ||...||::|:....:||::
  Rat   824 KWGIGFAKTFLLGLLRGKILSPKEIILSLGEPGGAGENAEESTPEDEKKEPPPEDKELKDNHILN 888

  Fly   701 ----------ALQAD----------GVHVE---------------------------------NG 712
                      :::.|          .:||.                                 :|
  Rat   889 HVGLTDGPRSSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDAFSEPEDIKKPLQPLYDG 953

  Fly   713 DGAVAPSKSKGKKKEEEKKEEEEVTEGPKPMLPYSSMFI---------LSPTN-----PIRRGAH 763
            :.:|..:......:|:.:::.||..||.:|...::...:         :|...     .:||...
  Rat   954 NSSVCSTADYKPPEEDPEEQAEENPEGEQPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACF 1018

  Fly   764 WVVNLPYFDFFIMVVISMSSIALAAEDPVRENSR-RNKILNYFDYAFTGVFTIEMLLKIVDLGVI 827
            .:|...:|:.||:.:|.:||.|||.||...|..| ...||.|.|..||.:|.:|||||.|..|..
  Rat  1019 KIVEHNWFETFIVFMILLSSGALAFEDIYIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFK 1083

  Fly   828 LHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKA 892
            :    |....|..:|.::|..:.:|...:..|.|   .|..|||||.||.||||:.:.|...::.
  Rat  1084 V----YFTNAWCWLDFLIVDVSIISLVANWLGYS---ELGPIKSLRTLRALRPLRALSRFEGMRV 1141

  Fly   893 VFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELL 957
            |.:.::.::.:::|:|:|.::|..|||::||.||.|||:||.:.:.....:......|.|.:.|:
  Rat  1142 VVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTSERFDISVVNNKSECESLM 1206

  Fly   958 PKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIV 1022
            ...::| |.....:||||....|:|..|.|.:||..::..::.:..::..|.....:.|.:::::
  Rat  1207 YTGQVR-WMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVNLYMYLYFVI 1270

  Fly  1023 YFIVFPFFFVNIFVALIIITFQEQ----GEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNT 1083
            :.|...||.:|:|:.:||..|.:|    |..::...|..|...:.:. .:|::..::.:|:.:|.
  Rat  1271 FIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMK-KLGSKKPQKPIPRPQNK 1334

  Fly  1084 FKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIG 1148
            .:..|:..|....|:..||:||..|.:.:|::..:|..:....|..|||.|..:|:.|.|||:
  Rat  1335 IQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKM-- 1397

  Fly  1149 FGVKN-FFKDPWNIFDLITVLGSIV-----DALWMEFGSNSINVGFLRLFRAARLIKLLRQGYTI 1207
            |.::: :|...|||||.:.|:.|||     |.:...|.|.:: ...:||.|..|:::|:|....|
  Rat  1398 FALRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVSPTL-FRVIRLARIGRVLRLIRGAKGI 1461

  Fly  1208 RILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENSITRHNNFQSFIQGVMLLF 1272
            |.||:..:.|..||..:.||:.::.|||:|.||..|..:|   .|:.|....||::|...::.||
  Rat  1462 RTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVK---KESGIDDMFNFETFGNSIICLF 1523

  Fly  1273 RCATGEAWPNIMLACLKG--KACDDDAEKAPGEY----CGS-TLAYAYFVSFIFFCSFLMLNLFV 1330
            ...|...|..::...|..  ..||...|. ||..    ||: ::...:|.|:|.....:::|:::
  Rat  1524 EITTSAGWDGLLNPILNSGPPDCDPTLEN-PGTNVRGDCGNPSIGICFFCSYIIISFLIVVNMYI 1587

  Fly  1331 AVIMDNFDYLTRDSS-ILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPN 1394
            |:|::||:..|.:|| .|.....:.|...|.::||:||..|.|:.:.|.:..:..||....  ||
  Rat  1588 AIILENFNVATEESSEPLSEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAK--PN 1650

  Fly  1395 RLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADME-LRETITNIWPLQAK 1458
            ::   |||.:::|:....::.....||||.:|.|.         |..:|: |::|      ::.|
  Rat  1651 KI---KLITLDLPMVPGDKIHCLDILFALTKEVLG---------DSGEMDALKQT------MEEK 1697

  Fly  1459 KMLNLLVPPSDQLNKGKLSVGKIYAGFLILESWRSTRFGQLDSGMPMLELQDA-SRHPSQESLTG 1522
            .|         ..|..|:|...|           :|...:....:..:::|.| .||..|.|:..
  Rat  1698 FM---------AANPSKVSYEPI-----------TTTLKRKQEEVCAIKIQRAYRRHLLQRSVKQ 1742

  Fly  1523 ADAGHLHPGHSYMNGHRRSPSLRHNGSPLARSPSPRRRGHQYIHHDIGFSDTVSNVVEMVKETRH 1587
            |         |||  :|.|.....:|:|.............|.|...|  |.|.:..|..|.:..
  Rat  1743 A---------SYM--YRHSQDGNGDGAPEKEGLLANTMNKMYGHEKEG--DGVQSQGEEEKASTE 1794

  Fly  1588 PRHGNSHPRYPRGSWSASTSPARSPSP 1614
            .......|: |..|...:.:|:..|.|
  Rat  1795 DAGPTVEPK-PTSSSDTALTPSPPPLP 1820

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
cac	NP_001356945.1	Ion_trans	37..324	CDD:334124	85/334 (25%)
		Ion_trans	441..677	CDD:334124	86/300 (29%)
		Ion_trans	769..1050	CDD:334124	91/285 (32%)
		Ion_trans	1094..1345	CDD:334124	81/263 (31%)
		GPHH	1346..1416	CDD:339854	18/69 (26%)
		Ca_chan_IQ	1418..1492	CDD:337190	16/74 (22%)
Scn4a	NP_037310.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	36..63
		I. /evidence=ECO:0000305	113..448		87/380 (23%)
		Ion_trans	130..451	CDD:395416	86/366 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	481..522		9/42 (21%)
		II. /evidence=ECO:0000305	554..826		87/289 (30%)
		Ion_trans	571..784	CDD:395416	73/225 (32%)
		Na_trans_assoc	810..1019	CDD:399489	23/208 (11%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	854..884		8/29 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	925..983		7/57 (12%)
		III. /evidence=ECO:0000305	1006..1319		96/321 (30%)
		Ion_trans	1023..1297	CDD:395416	90/281 (32%)
		Na_channel_gate	1289..1341	CDD:240441	9/52 (17%)
		Important for rapid channel inactivation. /evidence=ECO:0000269\|PubMed:28012039	1303..1305		0/1 (0%)
		IV. /evidence=ECO:0000305	1328..1626		93/304 (31%)
		Ion_trans	1346..1602	CDD:395416	81/262 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1775..1840		12/49 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG2301
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810