DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment cac and Cacna1c

DIOPT Version :9

Sequence 1:	NP_001356945.1	Gene:	cac / 32158	FlyBaseID:	FBgn0263111	Length:	2110	Species:	Drosophila melanogaster
Sequence 2:	NP_036649.2	Gene:	Cacna1c / 24239	RGDID:	2245	Length:	2170	Species:	Rattus norvegicus

Alignment Length:	2047	Identity:	866/2047 - (42%)
Similarity:	1165/2047 - (56%)	Gaps:	289/2047 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 GGPKKEENPPGGG------PTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEH 60
            |.|||:     ||      |.:|..||..||||:....|:||..||..:||||.||||.||:...
  Rat   117 GKPKKQ-----GGTTATRPPRALLCLTLKNPIRRACISIVEWKSFEIIILLTIFANCVALAIYIP 176

  Fly    61 LPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQY 125
            .|..|.......||:.|..||.||.|||.||::|.||:.|.::||||.||::||.:||.|..:..
  Rat   177 FPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAI 241

  Fly   126 PQ-------------IGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVL 177
            .:             .|...|::.|||.||||||:||||:|||||||.||||||.|||.|.||||
  Rat   242 LEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVL 306

  Fly   178 FAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLE 242
            |.|:|:||||||.:.|.:|||||:.|....:..| |..:||    .||...|. .|.|.| :|..
  Rat   307 FVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAE-EDPSPC----ALETGHGR-QCQNGT-VCKP 364

  Fly   243 KWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNL 307
            .|:||..|||:|||..||||||||||||||||.:|||..||:|....|:|||.|::.||||:|||
  Rat   365 GWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNL 429

  Fly   308 VLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIME 372
            |||||||||:|||||.:.|.:|.|||.:||||.:|.||::||.:||::    :...|:|.|.  |
  Rat   430 VLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMD--E 488

  Fly   373 ARRRNAAKRKKLKSLGKSKSTDTEEEEAEEDYGDD-GYLKTRSKPQGSCTGFWRAEKRF-RFWIR 435
            .:.||.:     ....:::|.:||.....:..|:: |........:...:.:||...|| |...|
  Rat   489 DKPRNMS-----MPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCR 548

  Fly   436 HTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIY 500
            ..||:..|||.||.||||||:.:|.|||.||.:|||....|....|.||.:||.:|||:||.:.|
  Rat   549 AAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAY 613

  Fly   501 FESSFNRFDCVVISGSIFEVIWSEVKGGS-FGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSM 564
            |.|.||||||.::.|.|.|.|..|.|..| .|:||||.:|||||||:|:||:||.|||.|||||:
  Rat   614 FVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSV 678

  Fly   565 RSIISLLFLLFLFILIFALLGMQLFGGQFNLPG-GTPETNFNTFPIALLTVFQILTGEDWNEVMY 628
            |||.|||.||||||:||:|||||||||:||... .|..:.|:.||.:||||||||||||||.|||
  Rat   679 RSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMY 743

  Fly   629 QGIISQGGAQ-KGMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQL 692
            .||::.||.. .||:..||||:|.:.|||.||||||||||||||:|:.||:|::|:.||.:.|:|
  Rat   744 DGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKL 808

  Fly   693 ---------QE-LEK-----------EMEALQADG-------VHVENGDGAVAPSKSKGKKKE-- 727
                     || :||           |::::.|||       :::::    :.||:::.|...
  Rat   809 ARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDD----LQPSENEDKSPHSN 869

  Fly   728 -----EEKKEEEEVTEGPK--------------PMLPYSSMFILSPTNPIRRGAHWVVNLPYFDF 773
                 ||.:||.|:..||:              ||...|:.||.||.|..|...|.:||...|..
  Rat   870 PDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTN 934

  Fly   774 FIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFW 838
            .|:..|.:|||:|||||||:..|.||.||.|||..||.:||||:.||:...|..||.||:.|.::
  Rat   935 LILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYF 999

  Fly   839 NIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKN 903
            ||:|.:||..:.:|||...|.      ::.:|.||||||||||:.|.|...||.|..||..:::.
  Rat  1000 NILDLLVVSVSLISFGIQSSA------INVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRT 1058

  Fly   904 VVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDEL-LPKQELRVWKP 967
            :.||:||..|.||:|:.||||||.||.:.|:|.||.|.|||:|:|..|::.|: .|..:.|.|:.
  Rat  1059 IGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWEN 1123

  Fly   968 RAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFV 1032
            ..|.:|||.|||:.||.|.|.||||::|..|:.:..||:|||.|:|:|:|||:|:|.|:..||.:
  Rat  1124 SKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMM 1188

  Fly  1033 NIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPF 1097
            ||||..:|:|||||||.|.::.|:||||:.|:::.:.||||.||:|||::  :||||.:|.||.|
  Rat  1189 NIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQH--QYKVWYVVNSTYF 1251

  Fly  1098 EYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIF 1162
            ||.:.:||:.||:.|.|:::.|..:::.::..:||.|||:|:||.:||:|.|..|::|.|.||.|
  Rat  1252 EYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDAWNTF 1316

  Fly  1163 DLITVLGSIVDALWMEF---------------GSNSINVGFLRLFRAARLIKLLRQGYTIRILLW 1212
            |.:.|:|||||....|.               .::.|::.|.||||..||:|||.:|..||.|||
  Rat  1317 DALIVVGSIVDIAITEVHPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLW 1381

  Fly  1213 TFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLG-TVENSITRHNNFQSFIQGVMLLFRCAT 1276
            ||::||:|||||.|||.|||||||:|||||||.|.|. |.|  |.|:||||:|.|.|:|||||||
  Rat  1382 TFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTE--INRNNNFQTFPQAVLLLFRCAT 1444

  Fly  1277 GEAWPNIMLACLKGKACDDDAE---KAPGEY-CGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNF 1337
            ||||.:|||||:.||.|..::|   ...||. |||:.|..||:||...|:||::|||||||||||
  Rat  1445 GEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNF 1509

  Fly  1338 DYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLI 1402
            ||||||.||||.||||||.||||||||.|.|:|.:.::..:|:.:.||||||..||:|:|.|:|:
  Rat  1510 DYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLV 1574

  Fly  1403 RMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADMELRETITNIWPLQAKKMLNLLVPP 1467
            .|||||:.:..|.|..|||||:|..|.||...  .::||:.|||..|..||...:.|:|:.:|||
  Rat  1575 SMNMPLNSDGTVMFNATLFALVRTALRIKTEG--NLEQANEELRAIIKKIWKRTSMKLLDQVVPP 1637

  Fly  1468 SDQLNKGKLSVGKIYAGFLILESWRSTRFGQLDSGMPMLELQDASRHPSQESLTGADAGHLHPGH 1532
            :   ...:::|||.||.|||.|.:|..:         ..:.|.....|||.:.....|| |...|
  Rat  1638 A---GDDEVTVGKFYATFLIQEYFRKFK---------KRKEQGLVGKPSQRNALSLQAG-LRTLH 1689

  Fly  1533 SYMNGHRRSPS------------LRHNGSPLARSPSPRRRGHQYIHHDIGF--SDTVSNVVEMVK 1583
            ......||:.|            ::...|..:.....||.|..:.:| :.:  ||:.||..:..
  Rat  1690 DIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNH-VSYYQSDSRSNFPQTF- 1752

  Fly  1584 ETRHPRHGNSHPRYPRGSWSAST-SPARSP------SPSRY-----GGHLSRSKRTQL---PYPT 1633
            .|:.|.|.|.     .|:..|.| ||:...      :||.|     ..:::.:..|.|   |:|.
  Rat  1753 ATQRPLHINK-----TGNNQADTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRFPHPA 1812

  Fly  1634 -YGTTSLCQRSRSP--SPARLQEMRERDRLGYGIDMGVTHVQHSYPTLASRR--------AGIGR 1687
             |.:|........|  |||                   ..||.:...|:|:|        |.:.:
  Rat  1813 GYSSTVSTVEGHGPPLSPA-------------------VRVQEAAWKLSSKRCHSRESQGATVSQ 1858

  Fly  1688 RLPPTPSKPSTLQLKPTNINFPKLNASPTHTHHSTPHSVHSLPHHRD-------LLRDPRDMYYS 1745
            .:.|..::.|.           :|:....:....:..|...|.:..|       |..|.|::..|
  Rat  1859 DMFPDETRSSV-----------RLSEEVEYCSEPSLLSTDILSYQDDENRQLTCLEEDKREIQPS 1912

  Fly  1746 SRERERDRERLRDRDRDRDRDRLHEYDLRYEYRDRERELYERERDREREVERERLEYIAPLSF-- 1808
            .:     |..||.....| |...|            .|..:|::|:..::.::.   ..||..
  Rat  1913 PK-----RSFLRSASLGR-RASFH------------LECLKRQKDQGGDISQKT---ALPLHLVH 1956

  Fly  1809 EQALAM---------------------------GRTGRVL-PSPV--LNGFKPKSGLNPR----- 1838
            .||||:                           |..||.| |.|.  |.|.:....||..
  Rat  1957 HQALAVAGLSPLLQRSHSPSTFPRPRPTPPVTPGSRGRPLQPIPTLRLEGAESSEKLNSSFPSIH 2021

  Fly  1839 -HSDSDEEDWCXQRLXLRSRNRKDQIWVXHQA 1869
             .|.|:|...|.....:..|.|...:.|..||
  Rat  2022 CSSWSEETTACSGGSSMARRARPVSLTVPSQA 2053

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
cac	NP_001356945.1	Ion_trans	37..324	CDD:334124	162/299 (54%)
		Ion_trans	441..677	CDD:334124	147/238 (62%)
		Ion_trans	769..1050	CDD:334124	138/281 (49%)
		Ion_trans	1094..1345	CDD:334124	144/270 (53%)
		GPHH	1346..1416	CDD:339854	39/69 (57%)
		Ca_chan_IQ	1418..1492	CDD:337190	31/73 (42%)
Cacna1c	NP_036649.2	Calmodulin-binding. /evidence=ECO:0000250\|UniProtKB:Q13936	77..98
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	104..128		6/15 (40%)
		I	141..438		161/303 (53%)
		Ion_trans	171..446	CDD:278921	151/281 (54%)
		TrbL	<232..>321	CDD:294888	48/88 (55%)
		Selectivity filter of repeat I. /evidence=ECO:0000250\|UniProtKB:P07293	391..394		2/2 (100%)
		AID/alpha-interaction domain, mediates interaction with the beta subunit. /evidence=ECO:0000269\|PubMed:15170217	458..475		9/16 (56%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	479..511		9/38 (24%)
		II	540..786		149/245 (61%)
		Ion_trans	571..794	CDD:278921	137/222 (62%)
		Selectivity filter of repeat II. /evidence=ECO:0000250\|UniProtKB:P07293	734..737		2/2 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	794..891		24/100 (24%)
		Interaction with STAC2. /evidence=ECO:0000250\|UniProtKB:Q13936	859..906		11/46 (24%)
		Ion_trans	947..1206	CDD:278921	132/264 (50%)
		Dihydropyridine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1119..1208		48/88 (55%)
		Selectivity filter of repeat III. /evidence=ECO:0000250\|UniProtKB:P07293	1143..1146		1/2 (50%)
		IV	1236..1509		142/276 (51%)
		Ion_trans	1266..1517	CDD:278921	135/252 (54%)
		Selectivity filter of repeat IV. /evidence=ECO:0000250\|UniProtKB:P07293	1444..1447		2/2 (100%)
		Dihydropyridine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1460..1528		41/67 (61%)
		Phenylalkylamine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1474..1516		28/41 (68%)
		GPHH	1525..1588	CDD:293510	33/62 (53%)
		Ca_chan_IQ	1590..1661	CDD:285917	32/75 (43%)
		Calmodulin-binding. /evidence=ECO:0000250\|UniProtKB:Q13936	1641..1674		11/41 (27%)
		Important for interaction with STAC1, STAC2 and STAC3. /evidence=ECO:0000250\|UniProtKB:P15381	1641..1668		10/35 (29%)
		Calmodulin-binding IQ region. /evidence=ECO:0000250\|UniProtKB:Q13936	1647..1667		8/28 (29%)
		Important for localization in at the junctional membrane. /evidence=ECO:0000250\|UniProtKB:P15381	1681..1700		6/19 (32%)
		CAC1F_C	1684..2117	CDD:293490	87/429 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1762..1794		8/36 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1971..1999		4/27 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D172471at2759
OrthoFinder	1	1.000	-	-		FOG0000179
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100303
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.820