Protein Alignment cac and Cacna1i

DIOPT Version :10

• Sequence 1: NP_001356945.1
• Gene: cac / 32158
• FlyBaseID: FBgn0263111
• Length: 2110
• Species: Drosophila melanogaster
• Sequence 2: XP_006521012.1
• Gene: Cacna1i / 239556
• MGIID: 2178051
• Length: 2226
• Species: Mus musculus
• Alignment Length: 2270
• Identity: 554/2270 - (24%)
• Similarity: 902/2270 - (39%)
• Gaps: 659/2270 - (29%)

  Fly     4 PKKEENP----PGGGPTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGG 64
            |....||    |...|.:.|.|.:....|.:...::..|.||...:|.|:.|||.|.:  :.|..
Mouse    39 PLDGTNPDVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECVSMLVILLNCVTLGM--YQPCD 101

  Fly    65 DKTVLAQK---LEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYP 126
            |...|:.:   |:..:.:....|.:|..||::||| :..|..||.:.||.:|||:|:.| |.:|.
Mouse   102 DMECLSDRCKILQVFDDFIFIFFAMEMVLKMVALG-IFGKKCYLGDTWNRLDFFIVMAG-MVEYS 164

  Fly   127 QIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFY 191
            .....::|..:|.:|||||||.::.:||:::::..::..:..|..:.||..|...||.|||::.:
Mouse   165 LDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLW 229

  Fly   192 SGALHKTCY-----------------------------SLEDPNKL--------VKEGESETPCN 219
            :|.|...|:                             ||...|.:        :||...|...:
Mouse   230 AGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLS 294

  Fly   220 TDNILEKATG------SFVC---NNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTA 275
            .|::.:...|      |.:|   |...::|......|:.|..:|||||:|.:.:||.||:|||..
Mouse   295 KDDMYDFGAGRQDLNASGLCVNWNRYYNVCRTGNANPHKGAINFDNIGYAWIVIFQVITLEGWVE 359

  Fly   276 ILYWTNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFA--KERE---KVENRQEFLKLRRQ 335
            |:|:..|| .|.:|:|||:.||::|||||:||.|.|::.:|:  |:||   .:|.||.:|.....
Mouse   360 IMYYVMDA-HSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTV 423

  Fly   336 QQLERELNGYVE---WIC----KAE---------------------------------------- 353
            .......:.|.|   ::|    ||:
Mouse   424 ASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALQNRRQATGPGTPAPAKPGPHAKEPSHCKLC 488

  Fly   354 -----------------EVILAEERTTEEEKMHIMEARRRNAAKRKKLKSLGKSKSTDTEEE--- 398
                             ..|||.:.::.....|  ||.||.:       .||   |||:.:|
Mouse   489 PRHSPLDTTPHTLVQPISAILASDPSSCPRCQH--EAGRRPS-------GLG---STDSGQEGSG 541

  Fly   399 -----EAE---------EDYGDDGYLKTRSKPQGS---CTGFWRAEKRFRFWIRHTVKTQWFYWF 446
                 |||         ||....|..|...:..|:   |...|| |.|.:  :|..|.:::|...
Mouse   542 SGGSAEAEANGDGPQSSEDGVSSGLGKEEEQEDGAARLCGDVWR-ETRAK--LRGIVDSKYFNRG 603

  Fly   447 VIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCV 511
            :::.:.:|||.:.:||:.||..||..|.....:|..:|..||.:|:.|.|...|..:.:|.||.:
Mouse   604 IMMAILVNTVSMGIEHHEQPEELTNILEICNVVFTSMFALEMILKLAAFGLFDYLRNPYNIFDSI 668

  Fly   512 VISGSIFEVIWSEVKGGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFL 576
            ::..||:|:: .:..|   ||||||..||||:.|:.::..:||..::.|:.:|.::.:...||.|
Mouse   669 IVIISIWEIV-GQADG---GLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLML 729

  Fly   577 FILIFALLGMQLFGGQFNLPGGTPET-----NFNTFPIALLTVFQILTGEDWNEVMYQGIISQGG 636
            ||.||::|||.:||.:|:|...|.:|     ||::...|::|||||||.||||.|:|.|:
Mouse   730 FIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGM----- 789

  Fly   637 AQKGMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEME- 700
            |......|:||:.|:.||||.|.|:.:||.|:..  ..|..|......|:.....|:||:|..|
Mouse   790 ASTTPWASLYFVALMTFGNYVLFNLLVAILVEGF--QAEGDANRSYSDEDQSSSNLEELDKLPEG 852

  Fly   701 ------------------------------------------ALQADGVHV-------------- 709
                                                      :||.|.|.|
Mouse   853 LDSSRDLKLCPIPMTPNGHLDPSLPLGGHLGPAGAMGAAPRLSLQPDPVLVALESRKSSVMSLGR 917

  Fly   710 --------------------------------------------------ENGDGAV-------- 716
                                                              |.|...|
Mouse   918 MSYDQRSLSSSRSSYYGPWGRSGTWASRRSSWNSLKHKPPSAEHESLLSGERGGSCVRACEGARE 982

  Fly   717 -APSK------------------------------------------------------------ 720
             ||.:
Mouse   983 DAPPRAAPLHAPHTHHAHHGPHLAHRHRHHRRTLSLDTRDSVDLAELVPVVGAHSRAAWRAAGQA 1047

  Fly   721 ------------------SKGKKKEEEKKEEEEV-----------TEGPKP----MLPYSSMFIL 752
                              :|...:.:..::|||:           .:..||    :....|:::.
Mouse  1048 PGHEDCNGRMPNIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLF 1112

  Fly   753 SPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDP-VRENSRRNKILNYFDYAFTGVFTIE 816
            ||.|..|.....::....||:.::..|.::.|.:|.|.| :...|.....|...:|.||.:|..|
Mouse  1113 SPENKFRILCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGE 1177

  Fly   817 MLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPL 881
            |.||:|.||:.....:|||..||::|..:|..:.:.....::.:...:.|..::.||:||.||||
Mouse  1178 MTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPL 1242

  Fly   882 KTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYC--TDESKHTS-AE 943
            :.|.|.|.||.|.:.:::|||.:.||:::...|..||.::|||||.|||::|  .|....|: ::
Mouse  1243 RVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSD 1307

  Fly   944 CQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGP 1008
            |..:.::              |....:::||:..|:::||.:.:.:||..::.:.:.|...|:.|
Mouse  1308 CVAANYR--------------WVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQP 1358

  Fly  1009 IQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITF---QEQGEAELQDGEIDKNQKSCIDFTIGA 1070
            :.|....|.:::|.:.::..||.:|:||.:::..|   ::..|||    |..:.::..:
Mouse  1359 VTNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAE----EARRREEKRL------ 1413

  Fly  1071 RPLERYMPKNRNTFKYKVW---RIVV-----STPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSL 1127
            |.||:...|.:....|..:   |:::     |...:.||..:|..|.:.:.::::||....|.:|
Mouse  1414 RRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETAL 1478

  Fly  1128 KYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNS---IN---V 1186
            ||.|..||.:|.:|.|||::.||::.||||.||..||..||.|::.....|...|:   ||   :
Mouse  1479 KYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTII 1543

  Fly  1187 GFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTV 1251
            ..:|:.|.||::|||:....:|.||.|.||:...:..:.||..:||||||.:|:::||.:....
Mouse  1544 RIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCND- 1607

  Fly  1252 EN---SITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYA- 1312
            ||   .::||..|::|....:.||:.:||:.|..||...|:.  |..|...     |.|:|.:.
Mouse  1608 ENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRD--CTHDERS-----CLSSLQFVS 1665

  Fly  1313 --YFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEM 1375
              |||||:....|:::|:.|||:|.:.|...::                |:.|.....:|.
Mouse  1666 PLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKE----------------AQEDAEMDAEIE---- 1710

  Fly  1376 YDMLKNMDP---------PLGFGNKCPN-----------------------------RLAYKK-- 1400
            .:|...:.|         |......||.                             |..|..
Mouse  1711 LEMAHGLGPGPGPCPCPCPCPCPCPCPGPRMPTSSPGAPGRGSGGAGVGGDTESHLCRHCYSPAQ 1775

  Fly  1401 ----LIRMNMPLDDELRVQFTTTLFALIRENLSIKM----RAPEEMDQADMELRETITNIWPLQA 1457
                |..:::.:.|.|..:.|      |.:|||..:    .:|...|:...:.:|     .|: |
Mouse  1776 ETLWLDSVSLIIKDSLEGELT------IIDNLSGSIFHHYSSPAGCDKCHHDKQE-----GPV-A 1828

  Fly  1458 KKMLNLLVPPSDQLNKG-------KLSVGKIYAGFLILESWRSTRFGQLDSGMPMLELQDASRHP 1515
            ::....|..||.::.:|       :|:..:.::    |.|.||:.....|.    |.|:|.:..|
Mouse  1829 QRSRPRLPHPSPRVTRGHHLPPQVQLAETEAFS----LNSDRSSSVLLGDD----LSLEDPTACP 1885

  Fly  1516 S--QESLTGADAGHLHPGHSYMNGHRRSPSLRHNGSPLARSPS---------PRRRGHQYIHHDI 1569
            .  :||     .|.|.|......|..........|.|::..|.         |......::.|:.
Mouse  1886 QGPKES-----KGELEPPEPMQAGDLDECFFPFAGEPVSAGPESLLCEMGAIPFNPVQSWLKHES 1945

  Fly  1570 G------FS-DTVSNVVEMVKETRHP---------RHGNSHPRYPR----GSWSASTSP------ 1608
            .      || |..|.:::|..|..||         ..|.|....|:    |||::..||
Mouse  1946 NQAPPSPFSPDGSSPLLQMPAEFFHPAVSASQKGQEPGMSSGTLPKIALQGSWASLRSPSVNCTL 2010

  Fly  1609 ---------ARSPSPSRYGGH----------LSRSKR------TQLPYPTYGTTSLCQRSRSPSP 1648
                     :...|||...|.          ||.|.|      .|:|.|        :.|.||:.
Mouse  2011 LRQATVSDTSLDASPSSSAGSLQTTLEDSLTLSDSPRRALGPPVQVPGP--------RASLSPAT 2067

  Fly  1649 ARLQEMRERDRL--------------GYGIDMGVTHVQHSYPTLASRRAGIGRR----------- 1688
            .|...:|.|...              |.....|.|:.....|:....|.|.|..
Mouse  2068 RRRLSLRGRGLFSLRGLRAHQRSHSSGGSTSPGCTYHDSMDPSDEEGRGGAGGGGAGSEHSETLS 2132

  Fly  1689 ---------LPPTPSKPSTLQLKPTNINFPKLNASPTHTHHSTPHSVHSLPHHRDLLRDP 1739
                     ||||        |.|..:. |....|.|.:..:.|....:....|.|.|.|
Mouse  2133 SLSLTSLFCLPPT--------LPPPGLT-PARKFSSTSSLAAGPGRPGATVSVRGLARSP 2183

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
cac	NP_001356945.1	Ion_trans	37..324	CDD:459842	106/340 (31%)
		Ion_trans	441..677	CDD:459842	89/240 (37%)
		Ion_trans	769..1050	CDD:459842	83/287 (29%)
		Ion_trans	1094..1345	CDD:459842	91/262 (35%)
		GPHH	1354..1407	CDD:465306	11/96 (11%)
		Ca_chan_IQ	1417..1496	CDD:462591	19/89 (21%)
		AF-4	<1512..>1704	CDD:461550	57/287 (20%)
Cacna1i	XP_006521012.1	Ion_trans	76..407	CDD:459842	105/335 (31%)
		Ion_trans	597..824	CDD:459842	88/237 (37%)
		Ion_trans	1128..1400	CDD:459842	82/285 (29%)
		Ion_trans	1445..1700	CDD:459842	91/278 (33%)
		PHA03307	1867..>2226	CDD:223039	71/343 (21%)