DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment cac and Scn1a

DIOPT Version :9

Sequence 1:	NP_001356945.1	Gene:	cac / 32158	FlyBaseID:	FBgn0263111	Length:	2110	Species:	Drosophila melanogaster
Sequence 2:	NP_001300926.1	Gene:	Scn1a / 20265	MGIID:	98246	Length:	2009	Species:	Mus musculus

Alignment Length:	1921	Identity:	468/1921 - (24%)
Similarity:	792/1921 - (41%)	Gaps:	494/1921 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYF 80
            ::|:|||..||:||....|:....|...::.||:.|||.:.: .:.|...|.|        |..|
Mouse   106 SALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTM-SNPPDWTKNV--------EYTF 161

  Fly    81 LCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRP 145
            ..|:..|:.:||:|.|..|...::||:.||.:||.|:...::|::..:|   ::..||..||||.
Mouse   162 TGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLG---NVSALRTFRVLRA 223

  Fly   146 LKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVK 210
            ||.:|.||.|:.::.::|:::..|..:.:|.:|.:.:||:|||:.:.|.|...|......|..::
Mouse   224 LKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLE 288

  Fly   211 EGESETPCNTD-------------------------NILEKATGSFVCNNTTS--------MCLE 242
            |...|.....|                         ..||....:.:|.|::.        ||::
Mouse   289 EHSIEKNITMDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVK 353

  Fly   243 KWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNL 307
            ....||.|.||||...:|.|::|:.:|.:.|..:...|..|.|..: .|:||.:|.:|||:::||
Mouse   354 AGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTY-MIFFVLVIFLGSFYLINL 417

  Fly   308 VLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTT--------- 363
            :|.|::..:.::.:..        |...:|.|.|....:|.:.|.:|.......||
Mouse   418 ILAVVAMAYEEQNQAT--------LEEAEQKEAEFQQMLEQLKKQQEAAQQAAATTASEHSREPS 474

  Fly   364 ----------EEEKMHIMEAR-RRNAAKRKKLK--SLGKSKSTD----TEEEE------------ 399
                      |..|:....|: |||..|::|.|  |.|:.|..|    :|.|:
Mouse   475 AAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIE 539

  Fly   400 ----------------------------------------------AEEDYGDD----------- 407
                                                          :|.|:.||
Mouse   540 GNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESR 604

  Fly   408 ----------GYLKTRSKPQGS--------------------CTGF------------------- 423
                      |..:..:..|.|                    |.|.
Mouse   605 RDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLP 669

  Fly   424 -------------------WRAEKRFRFWI-------------------------------RHTV 438
                               .|..:...|.:                               |...
Mouse   670 EVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKC 734

  Fly   439 KTQWF-------------YWF------------------VIVLVFLNTVCVAVEHYGQPSFLTEF 472
            ...|:             ||.                  :.:.:.|||:.:|:|||.........
Mouse   735 PPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHV 799

  Fly   473 LYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVKGGSFGLSVLRA 537
            |.....:|.|:|.:|||:|:.|:.|..||:..:|.||..:::.|:.|:..:.|:    ||||||:
Mouse   800 LTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVE----GLSVLRS 860

  Fly   538 LRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFN-------L 595
            .||||:||:.|.|.:|..|:..:.||:.::.:|..:|.:.:.|||::||||||..:.       .
Mouse   861 FRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIAT 925

  Fly   596 PGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKGMIYSIYFIVLVLFGNYTLLN 660
            ....|..:.|.|..:.|.||::|.|| |.|.|:..:...|.|   |..:::.:|:|: ||..:||
Mouse   926 DCKLPRWHMNDFFHSFLIVFRVLCGE-WIETMWDCMEVAGQA---MCLTVFMMVMVI-GNLVVLN 985

  Fly   661 VFLAI-----AVDNLANAQELTAAEEEQVEED-----------------------KEKQLQELE- 696
            :|||:     :.||||...:.......|:..|                       |:|.|.|::
Mouse   986 LFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVKKQKILDEIKP 1050

  Fly   697 --------------------KEMEALQ-ADGVHVENGDGA-----------------------VA 717
                                |:::.|: .:|.....|.|:                       ..
Mouse  1051 LDDLNNRKDNCISNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTV 1115

  Fly   718 P-----------------SKSKGKKKEEEKKEEEEVTEGPKPML--PYSSMFILSPTNPIR---- 759
            |                 |:|..::.:|:..|....:||....:  |.....::.|...:.
Mouse  1116 PIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVIEPEETLEPEAC 1180

  Fly   760 -------------------RGAHW---------VVNLPYFDFFIMVVISMSSIALAAEDPVRENS 796
                               ||..|         :|...:|:.||:.:|.:||.|||.||...:..
Mouse  1181 FTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQR 1245

  Fly   797 RRNK-ILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGS 860
            :..| :|.|.|..||.:|.:|||||.|..|.    .:|....|..:|.::|..:.||...:..|.
Mouse  1246 KTIKTMLEYADKVFTYIFILEMLLKWVAYGY----QTYFTNAWCWLDFLIVDVSLVSLTANALGY 1306

  Fly   861 SAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQL 925
            |   .|..|||||.||.||||:.:.|...::.|.:.::.::.:::|:|:|.::|..|||::||.|
Mouse  1307 S---ELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNL 1368

  Fly   926 FNGKFFYCTDESKHTSAECQGSYFKYEED-------ELLPKQELRVWKPRAFHYDNVAAAMLTLF 983
            |.|||::|.:.:       .|..|:..|.       :|:.:.|...||....::|||....|:|.
Mouse  1369 FAGKFYHCVNTT-------TGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLL 1426

  Fly   984 AVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF----PFFFVNIFVALIIITFQ 1044
            .|.|.:||..:    |.|..:.|......:.|.|::..:||::|    .||.:|:|:.:||..|.
Mouse  1427 QVATFKGWMDI----MYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFN 1487

  Fly  1045 EQ-----GEAELQDGEIDKNQKSCIDF--TIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIM 1102
            :|     |    ||..:.:.||...:.  .:|::..::.:|:..|.|:..|:..|....|:..||
Mouse  1488 QQKKKFGG----QDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIM 1548

  Fly  1103 MLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITV 1167
            :||..|.:.:|::..:|.|.....|..||:.|..:|:.|.|||:|... ..:|...|||||.:.|
Mouse  1549 ILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLR-HYYFTIGWNIFDFVVV 1612

  Fly  1168 LGSIVDALWME-----FGSNSINVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLL 1227
            :.|||.....|     |.|.:: ...:||.|..|:::|::....||.||:..:.|..||..:.||
Mouse  1613 ILSIVGMFLAELIEKYFVSPTL-FRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLL 1676

  Fly  1228 IAMLFFIYAIIGMQVFGNIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKA 1292
            :.::.|||||.||..|..:|.   |..|....||::|...::.||:..|...|..::...|..|.
Mouse  1677 LFLVMFIYAIFGMSNFAYVKR---EVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKP 1738

  Fly  1293 CDDDAEKA-PGEY----CGS-TLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSS-ILGAH 1350
            .|.|..|. ||..    ||: ::...:|||:|.....:::|:::|||::||...|.:|: .|...
Mouse  1739 PDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSED 1803

  Fly  1351 HLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQ 1415
            ..:.|..:|.::||:||..:.:.::......::|||....  ||:|   :||.|::|:....|:.
Mouse  1804 DFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQ--PNKL---QLIAMDLPMVSGDRIH 1863

  Fly  1416 FTTTLFALIRENLSIKMRAPEEMD--QADMELRETITNIWPLQAKKMLNLLVPPSDQLNKGKLSV 1478
            ....|||..:..|.    ...|||  :..||.|...:|...:..:.:...|....::::  .:.:
Mouse  1864 CLDILFAFTKRVLG----ESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVS--AVII 1922

  Fly  1479 GKIYAGFLILESWRSTRF----GQLDSGMPMLELQD 1510
            .:.|...|:..:.:...|    .:|..|..:|..:|
Mouse  1923 QRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKED 1958

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
cac	NP_001356945.1	Ion_trans	37..324	CDD:334124	87/319 (27%)
		Ion_trans	441..677	CDD:334124	85/278 (31%)
		Ion_trans	769..1050	CDD:334124	95/297 (32%)
		Ion_trans	1094..1345	CDD:334124	84/261 (32%)
		GPHH	1346..1416	CDD:339854	18/69 (26%)
		Ca_chan_IQ	1418..1492	CDD:337190	14/75 (19%)
Scn1a	NP_001300926.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	28..60
		I. /evidence=ECO:0000250\|UniProtKB:P04774	110..454		100/364 (27%)
		Ion_trans	127..433	CDD:366146	87/318 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	455..528		18/72 (25%)
		Na_trans_cytopl	510..718	CDD:371806	17/207 (8%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	584..627		6/42 (14%)
		II. /evidence=ECO:0000250\|UniProtKB:P04774	750..1022		86/280 (31%)
		Ion_trans	767..980	CDD:366146	71/221 (32%)
		Na_trans_assoc	1006..1213	CDD:368948	23/206 (11%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1129..1163		6/33 (18%)
		III. /evidence=ECO:0000250\|UniProtKB:P04774	1200..1514		103/335 (31%)
		Ion_trans	1217..1492	CDD:366146	94/292 (32%)
		Na_channel_gate	1484..1536	CDD:240441	12/55 (22%)
		IV. /evidence=ECO:0000250\|UniProtKB:P04774	1523..1821		96/302 (32%)
		Ion_trans	1540..1796	CDD:366146	84/260 (32%)
		S1-S2 loop of repeat IV. /evidence=ECO:0000269\|PubMed:27281198	1561..1571		2/9 (22%)
		S3b-S4 loop of repeat IV. /evidence=ECO:0000269\|PubMed:27281198	1619..1636		3/17 (18%)
		GPHH	1808..1855	CDD:374878	15/51 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1984..2009
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG2301
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810