Protein Alignment Upf1 and DNA2

DIOPT Version :10

• Sequence 1: NP_572767.1
• Gene: Upf1 / 32153
• FlyBaseID: FBgn0030354
• Length: 1180
• Species: Drosophila melanogaster
• Sequence 2: NP_012034.1
• Gene: DNA2 / 856569
• SGDID: S000001207
• Length: 1522
• Species: Saccharomyces cerevisiae
• Alignment Length: 1168
• Identity: 238/1168 - (20%)
• Similarity: 411/1168 - (35%)
• Gaps: 397/1168 - (33%)

  Fly    19 DNELLPGADTQPTQYDYRDFTMPSTSQSQTQNDQLEIA------QRCSAGDSHPRLASITNDLAD 77
            :|:.|...|..|              ::|..||.|.:.      ...|.|.|   :..:...:..
Yeast   479 ENKRLLSDDKNP--------------KTQLANDNLLVLNPDVLFSATSVGSS---VGCLRRSILQ 526

  Fly    78 LQFEEEDDEPG-----SSYVKELPPHACKYCGIHDPA------------------TVVMCNNCRK 119
            :||::...||.     .:.|.||...:.||...|:..                  ::::||...:
Yeast   527 MQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQ 591

  Fly   120 WFCNGRGSTSGSHIINHLVRAKHREVTLHGEGP-LGETILECYS--CGVRNVFVLGFIPAKADSV 181
            :             :..||..:|.|..|:.... :.::...||:  .|.|..
Yeast   592 Y-------------VKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRT------------- 630

  Fly   182 VVLLCRQPCAAQNSLKDMNWDQEQWKPLIADRCFLAWLVKQ----------PSEQGQLRARQIS- 235
                  ||.:..|.:   :.::..|.|:...:.||...|:.          |.|....::|.:|
Yeast   631 ------QPISISNVI---DIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSY 686

  Fly   236 -------AAQINKLEELWKENIEATF---QDLEK-PGIDSEPAHVL-----LRYEDGYQYEKTFG 284
                   ...:|...|:..|.....|   :::.| |.:.....|:|     :.....:|.::.||
Yeast   687 EVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFG 751

  Fly   285 ---------PLVRLEAEYD-----------QKLKESATQEN---IEVRWDVGLN--KKTIA---- 320
                     ||:| ::..|           .||.|..|.|.   :|..:::..|  .:.:|
Yeast   752 QAQSRFELPPLLR-DSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKE 815

  Fly   321 YFT-----LAKTDS-------DMKLMHG----------------DELRLHYVGE---LY------ 348
            :||     :.|.:|       ::.|:.|                .|:..|...|   :|
Yeast   816 FFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRR 880

  Fly   349 ---NPWSEIGHVIK------VPDNFGD--------------DVGLELKSSTNAPVKCTSNFTVDF 390
               |..|.:...|.      :.|..|.              .:|:.:|.      |..:|..:|.
Yeast   881 NDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKR------KLLNNRLLDK 939

  Fly   391 IWKCTSFDRM------------TRALCKFAIDRN------SVSNF----IYSRLLGHG------- 426
            ....|:...:            |:.|..:.||:|      |::.|    ::...:..|
Yeast   940 EKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDER 1004

  Fly   427 --------RADSNDEV----LFRGPQPKLFSA---PHLP-------DLNRSQVYAVKHALQ-RPL 468
                    |:|..:|:    |.....||...|   |.:|       .||.:|..|:...:: ...
Yeast  1005 SKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDY 1069

  Fly   469 SLIQGPPGTGKTVTSATIVYQLVKLHGGTVLVCAPSNTAVDQLTEKIHRTNLKVVRVCAKSREAI 533
            :||.|.||||||...|.|:..||. .|..||:.:.:::|||.:..|:..||:.::|:..|.:
Yeast  1070 ALILGMPGTGKTTVIAEIIKILVS-EGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHK--- 1130

  Fly   534 DSPVSFLALHNQIRNMETNSELKKLQQLKDETGELSSADEKRYRNLKRAAENQLLEAADVICCTC 598
                    :|     .:|...:.....:|.....||.                 :.:..|:..||
Yeast  1131 --------VH-----PDTQKYVPNYASVKSYNDYLSK-----------------INSTSVVATTC 1165

  Fly   599 VGAGD--GRLSRVKFTSILIDESMQSTEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAARAGL 661
            :|..|  ..|:...|..:::||:.|.:.|..:.|:..| .:.|:||||.||.|:|....|...||
Yeast  1166 LGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYG-NRFIMVGDHYQLPPLVKNDAARLGGL 1229

  Fly   662 SQSLFERLVVLGIRPF---------RLEVQYRMHPELSQFPSNFFYEGSLQ---NGVCAEDRRLK 714
            .:|||        :.|         .|.:||||..::....:...|:..|:   |.|.|:    .
Yeast  1230 EESLF--------KTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQ----S 1282

  Fly   715 LDFPWP---------------------QPERPMFFL--------VTQGQEEIAGSGTSFLNRTEA 750
            |:.|.|                     :|.|.:.||        :.|.:::      :..|..||
Yeast  1283 LELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKD------NITNHGEA 1341

  Fly   751 ANVEKITTRFLKAGIKPEQIGIITPYEGQRAYLVQYMQYQGSLHSRLYQEIEIASVDAFQGREKD 815
            ....:.....|.:|:..|.||::|.|..|...|.:.      .:..:|..:||.:.|.||||:|.
Yeast  1342 ELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKI------FNKNVYDGLEILTADQFQGRDKK 1400

  Fly   816 IIIMSCVRSNER-QGIGFLNDPRRLNVALTRAKFGIIIVGNPKVLAKQQLWNHLLNFYKDRKVLV 879
            .||:|.||.|.: .|...|.:.||:|||:||||..:||:|:...:
Yeast  1401 CIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTI-------------------- 1445

  Fly   880 EGSLNNLKESLIHFQKPKKLVNSMNIGAHFMSTIIADAKEVMVPGSIYDRSGGYGQGRQMVGQ 942
             ||:..:| |.::..:.:..|.:|             .|:.:......|||....:.|:..|:
Yeast  1446 -GSVPEIK-SFVNLLEERNWVYTM-------------CKDALYKYKFPDRSNAIDEARKGCGK 1493

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Upf1	NP_572767.1	UPF1_Zn_bind	98..249	CDD:401391	28/189 (15%)
		1B_UPF1-like	302..390	CDD:394815	24/156 (15%)
		TIGR00376	355..880	CDD:273041	147/640 (23%)
		DEXXQc_UPF1	451..684	CDD:350797	64/244 (26%)
DNA2	NP_012034.1	Dna2	444..662	CDD:462565	40/234 (17%)
		Cas4	<645..779	CDD:441077	24/134 (18%)
		TIGR00376	<1049..1464	CDD:273041	125/495 (25%)
		DEXXQc_DNA2	1052..1253	CDD:350799	64/243 (26%)