DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Met and Clock

DIOPT Version :9

Sequence 1:	NP_001285132.1	Gene:	Met / 32114	FlyBaseID:	FBgn0002723	Length:	716	Species:	Drosophila melanogaster
Sequence 2:	NP_001276755.1	Gene:	Clock / 12753	MGIID:	99698	Length:	855	Species:	Mus musculus

Alignment Length:	684	Identity:	137/684 - (20%)
Similarity:	239/684 - (34%)	Gaps:	242/684 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 REARNLAEKQRRDKLNASIQELATMVPHAAESSRRLDKTAVLRFATHGLRLQYVFGKSASRRRKK 102
            |.:||.:||:|||:.|..|:||.:|:|   .::|::||:.||:.:...|           |:.|:
Mouse    36 RVSRNKSEKKRRDQFNVLIKELGSMLP---GNARKMDKSTVLQKSIDFL-----------RKHKE 86

  Fly   103 TGLKGTGMS-ASPVGDLPNPSL----HLTDTLMQLLDCCFLTLTCSGQIVLVSTSVEQLLGHCQS 162
            |    |..| ||.:.....|:.    ..|..:::.||..||.:...|.|:.||.||..||.|..|
Mouse    87 T----TAQSDASEIRQDWKPTFLSNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTSLLEHLPS 147

  Fly   163 DLYGQNLLQITHPDDQDLLRQQLIPRDIETLFYQHQHHQQQGHNPQQHSTSTSASTSGSDLEEEE 227
            ||..|::.             ..||               :|.:.:.:...:      :.|.|.:
Mouse   148 DLVDQSIF-------------NFIP---------------EGEHSEVYKILS------THLLESD 178

  Fly   228 METEEHRLGRQQGEADDDEDHPYNRRTPSPRRMAHLATIDDRLRMDRRCFTVRLARASTRAEATR 292
            ..|.|:...:.|.|                                   |...:.|.:...:...
Mouse   179 SLTPEYLKSKNQLE-----------------------------------FCCHMLRGTIDPKEPS 208

  Fly   293 HYERVKIDGCFRRSDSSLTGGAAANYPIVSQLIRRSRNNNMLAAAAAVAAEAATVPPQHDAIAQA 357
            .||.|:..|.|:    |||..:.:.:......|:|:..                 |...|.:...
Mouse   209 TYEYVRFIGNFK----SLTSVSTSTHNGFEGTIQRTHR-----------------PSYEDRVCFV 252

  Fly   358 ALHGISGNDIVLVAMARVLRE----ERPPEETEGTVGLTIYRQPEPYQLEYHTRHLIDGSIIDCD 418
            |        .|.:|..:.::|    |.|.|                   |:.:||.::...:..|
Mouse   253 A--------TVRLATPQFIKEMCTVEEPNE-------------------EFTSRHSLEWKFLFLD 290

  Fly   419 QRIGLVAGYMKDEVRNLSPFCFMHLDDVRWVIVALRQMYDCNSDY-----GESC-YRLLSRNGRF 477
            .|...:.||:..||...|.:.:.|:||       |..:..|:...     |:|| ||.|::..::
Mouse   291 HRAPPIIGYLPFEVLGTSGYDYYHVDD-------LENLAKCHEHLMQYGKGKSCYYRFLTKGQQW 348

  Fly   478 IYLHTKGFLEVDRGSNKVHSFLCVNTLL---DEEAGRQKVQEMKEKFSTIIKAEMPTQSSSPDLP 539
            |:|.|..::...:.:::....:|.:|::   :..|.|::              |:..:.|.|:..
Mouse   349 IWLQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRR--------------ELGIEESLPETA 399

  Fly   540 ASQAPQQLERIVLYLIENLQKSVDSAETVGGQGMESLMDDGYSSPANTLTLEELAP----SPTPA 600
            |.::                                 .|.|..:..||::|:|...    ||||:
Mouse   400 ADKS---------------------------------QDSGSDNRINTVSLKEALERFDHSPTPS 431

  Fly   601 LA---------LVPPAPSSVKSSISKSVSVVNVTAARKFQQEHQKQRERDREQLKERTNSTQGVI 656
            .:         .....|||..:.|....|    |..|:....|:|..:| |.....::.::|.|
Mouse   432 ASSRSSRKSSHTAVSDPSSTPTKIPTDTS----TPPRQHLPAHEKMTQR-RSSFSSQSINSQSV- 490

  Fly   657 RQLSSCLSEAETASCILSPASSLSASEAPDTPDP 690
                        ...:..||    .|:|.:.|.|
Mouse   491 ------------GPSLTQPA----MSQAANLPIP 508

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Met	NP_001285132.1	HLH	38..87	CDD:238036	19/48 (40%)
		PAS	137..>186	CDD:238075	15/48 (31%)
		PAS_11	403..510	CDD:291273	26/115 (23%)
Clock	NP_001276755.1	bHLH-PAS_CLOCK	29..89	CDD:381577	23/70 (33%)
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:19414601	32..47		5/10 (50%)
		PAS	109..>177	CDD:366402	22/101 (22%)
		PAS_11	274..377	CDD:373151	27/128 (21%)
		Interaction with NR3C1. /evidence=ECO:0000269\|PubMed:19141540	371..854		37/207 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	392..411		5/51 (10%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	420..497		17/94 (18%)
		Interaction with SIRT1. /evidence=ECO:0000269\|PubMed:18662547	450..570		17/81 (21%)
		Implicated in the circadian rhythmicity	514..564
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	613..650
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	752..791
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	814..855
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167834653
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3561
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.740