DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpII215 and RPO31

DIOPT Version :9

Sequence 1:	NP_511124.1	Gene:	RpII215 / 32100	FlyBaseID:	FBgn0003277	Length:	1887	Species:	Drosophila melanogaster
Sequence 2:	NP_014759.1	Gene:	RPO31 / 854283	SGDID:	S000005642	Length:	1460	Species:	Saccharomyces cerevisiae

Alignment Length:	1589	Identity:	521/1589 - (32%)
Similarity:	796/1589 - (50%)	Gaps:	268/1589 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 SKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGR-PKLGGLMDPRQGVIDRTSRCQTC 70
            |:.| :::|.::|..||..:|...|..|...:....:|..| ||..|.:||:.||...:..|.||
Yeast     7 SETP-KRIKGLEFSALSAADIVAQSEVEVSTRDLFDLEKDRAPKANGALDPKMGVSSSSLECATC 70

  Fly    71 AGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRK 135
            .||:..|.|||||:.||.||||||:...||:||:.:|..||.:|:|..:.:.....::..|....
Yeast    71 HGNLASCHGHFGHLKLALPVFHIGYFKATIQILQGICKNCSAILLSETDKRQFLHELRRPGVDNL 135

  Fly   136 R----LAYVYDLCKGKTIC----------------EGGEDMDLTKE-----NQQPDPNKKPGHGG 175
            |    |..:.|.||.:..|                .|...:.:..:     .::..|.|
Yeast   136 RRMGILKKILDQCKKQRRCLHCGALNGVVKKAAAGAGSAALKIIHDTFRWVGKKSAPEK------ 194

  Fly   176 CGHYQPSIRRTGLDL-TAEWK----HQNE-DSQEKKIV--VSAERVWEILKHITDEECFILGMDP 232
                         |: ..|||    |..| :...|:.:  ::..:...:.|.|...:|.:||:|.
Yeast   195 -------------DIWVGEWKEVLAHNPELERYVKRCMDDLNPLKTLNLFKQIKSADCELLGIDA 246

  Fly   233 KY--ARPDWMIVTVLPVPPLAVRPAVVMFGA-AKNQDDLTHKLSDIIKANNELRKNEASGAAAHV 294
            ..  .||:..|...||.||:.:||:|:|..: |.|:||||.||::|:..::.::.....|.:.:.
Yeast   247 TVPSGRPETYIWRYLPAPPVCIRPSVMMQDSPASNEDDLTVKLTEIVWTSSLIKAGLDKGISINN 311

  Fly   295 IQENIKMLQFHVATLVDNDM--PGMPRAMQKSG---KPLKAIKARLKGKEGRIRGNLMGKRVDFS 354
            :.|:...||..||..:::|.  |.|.......|   ||::....|||||:||.||||.|||||||
Yeast   312 MMEHWDYLQLTVAMYINSDSVNPAMLPGSSNGGGKVKPIRGFCQRLKGKQGRFRGNLSGKRVDFS 376

  Fly   355 ARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQYPGAKYIVRDNGE-R 418
            .||||:|||||.||:|.||..:|:.||:||.||.:|..::|||:..|.:.:|||.|:::.|.: |
Yeast   377 GRTVISPDPNLSIDEVAVPDRVAKVLTYPEKVTRYNRHKLQELIVNGPNVHPGANYLLKRNEDAR 441

  Fly   419 IDLRFHPKSS-DLHLQCGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLPWSTFRMNLSCTS 482
            .:||:..:.. ..:||.|..|||||.|.|:|:|||||:||::|::.|..|:.||.|||:|....:
Yeast   442 RNLRYGDRMKLAKNLQIGDVVERHLEDGDVVLFNRQPSLHRLSILSHYAKIRPWRTFRLNECVCT 506

  Fly   483 PYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVFI 547
            |||||||||||||||||:.|.|||..|:......::||::.:|::...||.:|....::.:|.|.
Yeast   507 PYNADFDGDEMNLHVPQTEEARAEAINLMGVKNNLLTPKSGEPIIAATQDFITGSYLISHKDSFY 571

  Fly   548 TREQVMNLLMFLPT----WDAKMPQPCILKPRPLWTGKQIFSLIIPGN------VNMIRTHSTHP 602
            .|..:..||..:..    :|  :|.|.|:||..||||||:|||:|..|      :|:...:....
Yeast   572 DRATLTQLLSMMSDGIEHFD--IPPPAIMKPYYLWTGKQVFSLLIKPNHNSPVVINLDAKNKVFV 634

  Fly   603 DEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLELGHD------------ 655
            ..:.:.....:|..|..|::...:::.|::.|..||...   .|..|..:..|
Yeast   635 PPKSKSLPNEMSQNDGFVIIRGSQILSGVMDKSVLGDGK---KHSVFYTILRDYGPQEAANAMNR 696

  Fly   656 ---IAGRFYGNIQTVINNWLLFEGHSIGIGD-TIAD--PQTYNEIQQAIKKAKDDVINVIQKAHN 714
               :..||.||           .|.||||.| |.||  .|...|:.:......|::|.:..|.
Yeast   697 MAKLCARFLGN-----------RGFSIGINDVTPADDLKQKKEELVEIAYHKCDELITLFNKG-- 748

  Fly   715 MELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIAC 779
             |||..||....||.|.|:..:|:..|::.|......|..:|....|...|||||.:|:||::|.
Yeast   749 -ELETQPGCNEEQTLEAKIGGLLSKVREEVGDVCINELDNWNAPLIMATCGSKGSTLNVSQMVAV 812

  Fly   780 VGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAV 844
            ||||.:.|.|:|.||:.|:||||.|:...|:|:|||.||:.:||:|.||.|||:.|||||:||||
Yeast   813 VGQQIISGNRVPDGFQDRSLPHFPKNSKTPQSKGFVRNSFFSGLSPPEFLFHAISGREGLVDTAV 877

  Fly   845 KTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFE 909
            |||||||:.|||:|::|.:...||.|||.|...::|..||.|||  :.:|.:........|:|::
Yeast   878 KTAETGYMSRRLMKSLEDLSCQYDNTVRTSANGIVQFTYGGDGL--DPLEMEGNAQPVNFNRSWD 940

  Fly   910 KRFKFDWSN----------------------ERLMKKVFTDDVIK-EMTDSSEAIQELEAEWDRL 951
            ..:...::|                      |||::...:..::| |..:.:|.:.:.:||
Yeast   941 HAYNITFNNQDKGLLPYAIMETANEILGPLEERLVRYDNSGCLVKREDLNKAEYVDQYDAE---- 1001

  Fly   952 VSDRD---SLRQIFPNGESKVVLPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCV 1013
               ||   |||: :.||::                          |.|:.:|..:|:..|||
Yeast  1002 ---RDFYHSLRE-YINGKA--------------------------TALANLRKSRGMLGLLE--- 1033

  Fly  1014 IVTGNDRISKQANENATLLFQCLIRSTLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEM 1078
                     ..|.|...:.....:...:.|. ||:.:|:|.::....:.....::::|:..||..
Yeast  1034 ---------PPAKELQGIDPDETVPDNVKTS-VSQLYRISEKSVRKFLEIALFKYRKARLEPGTA 1088

  Fly  1079 VGALAAQSLGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDA 1143
            :||:.|||:|||.|||||.|||||||:|.||||||||:|||||.||....|.:...|...  .|.
Yeast  1089 IGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINASKVISTPIINAVLVND--NDE 1151

  Fly  1144 EKAKNVLCRLEHTTLRKVTANTAIYYDPDPQRTVISEDQEFVNVYYEMPDFDPTRISPWLLRIEL 1208
            ..|:.|..|:|.|.|..|.     :|..|    |..::..|:.|..::...|         :::|
Yeast  1152 RAARVVKGRVEKTLLSDVA-----FYVQD----VYKDNLSFIQVRIDLGTID---------KLQL 1198

  Fly  1209 DRKRMTDKKLTMEQI------AEKINVGFGEDLNCIFNDDNADKLVLRIRIMNNEENKFQD---- 1263
            :        ||:|.|      |.|:.:. ..|:|.|..|        ||.|     |.|.:
Yeast  1199 E--------LTIEDIAVAITRASKLKIQ-ASDVNIIGKD--------RIAI-----NVFPEGYKA 1241

  Fly  1264 ---EDEAVDKMEDDMFLRCIE-ANMLSDMTLQGIEAIGKVYMHLPQTDSKKRIVITETGEFKAIG 1324
               ...|.:..|:|:|.|..: ...|.|:.::|:..|.:..::: :.|.|:.:::
Yeast  1242 KSISTSAKEPSENDVFYRMQQLRRALPDVVVKGLPDISRAVINI-RDDGKRELLV---------- 1295

  Fly  1325 EWLLETDGTSMMKVLSERDVDPIRTSSNDICEIFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRH 1389
                  :|..:..|:....|...||::|.:.|:|.||||||.|.|:.:|:|..:..:|:.|:.||
Yeast  1296 ------EGYGLRDVMCTDGVIGSRTTTNHVLEVFSVLGIEAARYSIIREINYTMSNHGMSVDPRH 1354

  Fly  1390 LALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVLMDAAAHAETDPMRGVSENIIMGQ 1454
            :.||.||||.||.::.|||.|:::.....|...|||:|.|.|.|||.:.:.|.:.||||.||:||
Yeast  1355 IQLLGDVMTYKGEVLGITRFGLSKMRDSVLQLASFEKTTDHLFDAAFYMKKDAVEGVSECIILGQ 1419

  Fly  1455 LPKMGTGCFDLL---------LDAEKCRF 1474
            ...:|||.|.::         |..::|.|
Yeast  1420 TMSIGTGSFKVVKGTNISEKDLVPKRCLF 1448

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpII215	NP_511124.1	PRK08566	14..887	CDD:236292	341/944 (36%)
		RNAP_II_RPB1_N	15..868	CDD:259848	332/924 (36%)
		RNA_pol_Rpb1_5	822..1420	CDD:282807	190/637 (30%)
		RNA_pol_Rpb1_6	890..1071	CDD:282801	33/206 (16%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	141/440 (32%)
		TCRP1	1602..1880	CDD:291605
		RCR	<1789..1864	CDD:304939
RPO31	NP_014759.1	RNAP_III_RPC1_N	21..905	CDD:259847	333/921 (36%)
		rpoC2	789..>1114	CDD:235052	128/373 (34%)
		RNAP_III_Rpc1_C	1077..1428	CDD:132723	138/409 (34%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0086
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000819
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100210
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.710