DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpII215 and NRPB1

DIOPT Version :9

Sequence 1:	NP_511124.1	Gene:	RpII215 / 32100	FlyBaseID:	FBgn0003277	Length:	1887	Species:	Drosophila melanogaster
Sequence 2:	NP_001329346.1	Gene:	NRPB1 / 829734	AraportID:	AT4G35800	Length:	1839	Species:	Arabidopsis thaliana

Alignment Length:	1905	Identity:	1056/1905 - (55%)
Similarity:	1351/1905 - (70%)	Gaps:	133/1905 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 SKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTCA 71
            |.|.:.:|:.||||||||||||:|||..  |:.:||.|.|:||:|||.|.|.|.|||..:|:||.
plant     8 SPAEVSKVRVVQFGILSPDEIRQMSVIH--VEHSETTEKGKPKVGGLSDTRLGTIDRKVKCETCM 70

  Fly    72 GNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRKR 136
            .||.|||||||:::||||::|:||:...:.|:|||||.|||:|......|.|: .||.: .|:.|
plant    71 ANMAECPGHFGYLELAKPMYHVGFMKTVLSIMRCVCFNCSKILADEEEHKFKQ-AMKIK-NPKNR 133

  Fly   137 LAYVYDLCKGKTICEGGEDMDLTKENQQPDPNKKPGHGGCGHYQPSIRRTGLDLTAEWKHQNE-- 199
            |..:.|.||.||.|:||:|:|..:.:...:|.|| ..||||..||.:...|:.:.||:|.|.:
plant   134 LKKILDACKNKTKCDGGDDIDDVQSHSTDEPVKK-SRGGCGAQQPKLTIEGMKMIAEYKIQRKKN 197

  Fly   200 -------DSQEKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVV 257
                   :..|:|..:.|:||..:||.|:|.:|.:||.:||:|||||||:.|||:||..|||:|:
plant   198 DEPDQLPEPAERKQTLGADRVLSVLKRISDADCQLLGFNPKFARPDWMILEVLPIPPPPVRPSVM 262

  Fly   258 MFGAAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQ 322
            |...::::|||||:|:.||:.|..|::.|.:||.||:|.|..::||||:||..||::||.|||.|
plant   263 MDATSRSEDDLTHQLAMIIRHNENLKRQEKNGAPAHIISEFTQLLQFHIATYFDNELPGQPRATQ 327

  Fly   323 KSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVT 387
            |||:|:|:|.:|||.||||||||||||||||||||||||||.:.||::|||.|||.|||:||.||
plant   328 KSGRPIKSICSRLKAKEGRIRGNLMGKRVDFSARTVITPDPTINIDELGVPWSIALNLTYPETVT 392

  Fly   388 PFNIDRMQELVRRGNSQYP---GAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVI 449
            |:||:|::|||..|....|   |||||:||:|:|:|||:..||||.||:.||||||||:|.|.|:
plant   393 PYNIERLKELVDYGPHPPPGKTGAKYIIRDDGQRLDLRYLKKSSDQHLELGYKVERHLQDGDFVL 457

  Fly   450 FNRQPTLHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITP 514
            |||||:|||||:||||::::|:||||:|||.||||||||||||||:|||||.||||||..:.:.|
plant   458 FNRQPSLHKMSIMGHRIRIMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSFETRAEVLELMMVP 522

  Fly   515 RQIITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWT 579
            :.|::||||:||||||||||...||:||||.||.::..||.||:...:|.|:|.|.|||||||||
plant   523 KCIVSPQANRPVMGIVQDTLLGCRKITKRDTFIEKDVFMNTLMWWEDFDGKVPAPAILKPRPLWT 587

  Fly   580 GKQIFSLIIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSL 644
            |||:|:||||..:|::|..:.|.|.|.    .:|:||||:|.:|.|||:.|.||||:||||.|||
plant   588 GKQVFNLIIPKQINLLRYSAWHADTET----GFITPGDTQVRIERGELLAGTLCKKTLGTSNGSL 648

  Fly   645 LHICFLELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVI 709
            :|:.:.|:|.|.|.:|.|:.|.::|.|||..|.:|||||||||..|..:|.:.|..||..|.::|
plant   649 VHVIWEEVGPDAARKFLGHTQWLVNYWLLQNGFTIGIGDTIADSSTMEKINETISNAKTAVKDLI 713

  Fly   710 QKAHNMELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINIS 774
            ::....||:|.||.|:|.||||:||::||.|||..|.||:|||.|.|||||||.:|||||.||||
plant   714 RQFQGKELDPEPGRTMRDTFENRVNQVLNKARDDAGSSAQKSLAETNNLKAMVTAGSKGSFINIS 778

  Fly   775 QVIACVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGL 839
            |:.||||||||||||||:||..||||||.|||||||||||||||||.||||.||:||||||||||
plant   779 QMTACVGQQNVEGKRIPFGFDGRTLPHFTKDDYGPESRGFVENSYLRGLTPQEFFFHAMGGREGL 843

  Fly   840 IDTAVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLS 904
            |||||||:|||||||||:||||.:||.||||||||:|.:||..|||||:....:|.|.:.::|:.
plant   844 IDTAVKTSETGYIQRRLVKAMEDIMVKYDGTVRNSLGDVIQFLYGEDGMDAVWIESQKLDSLKMK 908

  Fly   905 NKSFEKRFKFDWSNERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSL-RQIFPNGESK 968
            ...|::.||::..:|.......:|:.::::....|.....:||:.:|.:||..| .:|..||:|.
plant   909 KSEFDRTFKYEIDDENWNPTYLSDEHLEDLKGIRELRDVFDAEYSKLETDRFQLGTEIATNGDST 973

  Fly   969 VVLPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLF 1033
            ..||.|::|.|||.||.|.|:.|..:|:.|:.::..|..|.||.::|.|:|.:|.:|.:||||.|
plant   974 WPLPVNIKRHIWNAQKTFKIDLRKISDMHPVEIVDAVDKLQERLLVVPGDDALSVEAQKNATLFF 1038

  Fly  1034 QCLIRSTLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNT 1098
            ..|:||||.:|.|.||::||.|||||::||||:||.|:...||||:|.:||||:|||||||||||
plant  1039 NILLRSTLASKRVLEEYKLSREAFEWVIGEIESRFLQSLVAPGEMIGCVAAQSIGEPATQMTLNT 1103

  Fly  1099 FHFAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTA 1163
            ||:||||:||||||||||:||||::|:.|.|||:|:||..|::..|.||.|.|.||:||||.||.
plant  1104 FHYAGVSAKNVTLGVPRLREIINVAKRIKTPSLSVYLTPEASKSKEGAKTVQCALEYTTLRSVTQ 1168

  Fly  1164 NTAIYYDPDPQRTVISEDQEFVNVYYEMPDFD--PTRISPWLLRIELDRKRMTDKKLTMEQIAEK 1226
            .|.::|||||..|:|.||.|||..||||||.|  |.:||||||||||:|:.|.||||:|..||||
plant  1169 ATEVWYDPDPMSTIIEEDFEFVRSYYEMPDEDVSPDKISPWLLRIELNREMMVDKKLSMADIAEK 1233

  Fly  1227 INVGFGEDLNCIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQ 1291
            ||:.|.:||.||||||||.||:|||||||:|..|.:.:||:.   |||:||:.||:|||::|.|:
plant  1234 INLEFDDDLTCIFNDDNAQKLILRIRIMNDEGPKGELQDESA---EDDVFLKKIESNMLTEMALR 1295

  Fly  1292 GIEAIGKVYMHLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICE 1356
            ||..|.||::   :...|.|  ..|.|.||...||:|:|:|.:::.|:...||||.||:||.:.|
plant  1296 GIPDINKVFI---KQVRKSR--FDEEGGFKTSEEWMLDTEGVNLLAVMCHEDVDPKRTTSNHLIE 1355

  Fly  1357 IFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMR 1421
            |.:||||||||:::..|:..|:.|.|.||||||||:|||.||.:|||||||||||||.|||.|||
plant  1356 IIEVLGIEAVRRALLDELRVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPLMR 1420

  Fly  1422 CSFEETVDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIEIPNTLGNSM 1486
            ||||||||:|:||||:||||.:|||:|||::|||..:|||..:|.|:.|..:..||:  .|.:.|
plant  1421 CSFEETVDILLDAAAYAETDCLRGVTENIMLGQLAPIGTGDCELYLNDEMLKNAIEL--QLPSYM 1483

  Fly  1487 LGGAAMFIGGGSTPSMTPPM-TPW-ANCNTPRY-FSPPGHVSAMTP-------GGPSFSPSAASD 1541
            .|     :..|.||:.:|.. ||: ....:|.| .||...:|.|:.       ||.:||||::
plant  1484 DG-----LEFGMTPARSPVSGTPYHEGMMSPNYLLSPNMRLSPMSDAQFSPYVGGMAFSPSSS-- 1541

  Fly  1542 ASGMSPSWSPAHPGSSPSSPGPSMSPYFPASPSVSPSYSPTSPNYTASSPGGASPNYSPSSPNYS 1606
                        ||.||||||                ||||||.|:.:|||     |||:||.||
plant  1542 ------------PGYSPSSPG----------------YSPTSPGYSPTSPG-----YSPTSPGYS 1573

  Fly  1607 PTSPLYASPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQSSPSFAGSGSNIYSPGNAY 1671
            ||||.                   |||||.|||||||.||||     |||              |
plant  1574 PTSPT-------------------YSPSSPGYSPTSPAYSPT-----SPS--------------Y 1600

  Fly  1672 SPSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTPSYSPTSPNYS- 1735
            ||:|.:|||.|||||||||||||:|||||||||.||||||:||||||.|:|.:|:||||||:||
plant  1601 SPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSP 1665

  Fly  1736 ASPQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGSPGSPQYTPGSPQYSPASPKYSPTSPLYSP 1800
            .||.|||.||:||.|...||||||:|||.||:|  ||.||.|:|.||.|||.||.|.||||.|:|
plant  1666 TSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAY--SPTSPGYSPTSPSYSPTSPSYGPTSPSYNP 1728

  Fly  1801 SSPQHSPSNQYSPT------GSTYSATSPRYSPNMSIYSPSSTKYSPTSPTYTPTARNYSPTSPM 1859
            .|.::|||..|||:      .|.||.|||.|||....|||:|..|||:||||:|::...|..||.
plant  1729 QSAKYSPSIAYSPSNARLSPASPYSPTSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYSSGASPD 1793

  Fly  1860 YSPTAPSHYSPTSPAYSPSS 1879
            |||:|  .||||.|.|||||
plant  1794 YSPSA--GYSPTLPGYSPSS 1811

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpII215	NP_511124.1	PRK08566	14..887	CDD:236292	527/884 (60%)
		RNAP_II_RPB1_N	15..868	CDD:259848	512/864 (59%)
		RNA_pol_Rpb1_5	822..1420	CDD:282807	334/600 (56%)
		RNA_pol_Rpb1_6	890..1071	CDD:282801	69/181 (38%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	257/419 (61%)
		TCRP1	1602..1880	CDD:291605	166/285 (58%)
		RCR	<1789..1864	CDD:304939	42/80 (53%)
NRPB1	NP_001329346.1	RNAP_II_RPB1_N	18..872	CDD:259848	512/862 (59%)
		RNA_pol_Rpb1_6	892..1076	CDD:398590	69/183 (38%)
		RNAP_II_Rpb1_C	1055..1467	CDD:132720	257/419 (61%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	654	1.000	Domainoid score	I25
eggNOG	1	0.900	-	-		E1_COG0086
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H721
Inparanoid	1	1.050	1861	1.000	Inparanoid score	I4
OMA	1	1.010	-	-		QHG53835
OrthoDB	1	1.010	-	-		D591636at2759
OrthoFinder	1	1.000	-	-		FOG0000819
OrthoInspector	1	1.000	-	-		oto3089
orthoMCL	1	0.900	-	-		OOG6_100210
Panther	1	1.100	-	-	LDO	PTHR19376
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X2195
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.840