DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpII215 and POLR2A

DIOPT Version :9

Sequence 1:	NP_511124.1	Gene:	RpII215 / 32100	FlyBaseID:	FBgn0003277	Length:	1887	Species:	Drosophila melanogaster
Sequence 2:	NP_000928.1	Gene:	POLR2A / 5430	HGNCID:	9187	Length:	1970	Species:	Homo sapiens

Alignment Length:	1926	Identity:	1404/1926 - (72%)
Similarity:	1640/1926 - (85%)	Gaps:	54/1926 - (2%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 DSKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTC 70
            ||..|||.:||||||:|||||::||||||||:::.||.||||||||||||||||||:||.|||||
Human    10 DSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTC 74

  Fly    71 AGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRK 135
            ||||||||||||||:|||||||:||:.||:|:||||||:|||:||..:|||||:|:.||:|||:|
Human    75 AGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKK 139

  Fly   136 RLAYVYDLCKGKTICEGGEDMDLTKENQQP----DPNKKPGHGGCGHYQPSIRRTGLDLTAEWKH 196
            ||.:||||||||.||||||:||.....:||    |..|:.||||||.|||.|||:||:|.|||||
Human   140 RLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKH 204

  Fly   197 QNEDSQEKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVVMFGA 261
            .|||||||||::|.|||.||.|.|:|||||:|||:|:||||:||||||||||||:|||||||.|:
Human   205 VNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGS 269

  Fly   262 AKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQKSGK 326
            |:|||||||||:||:|.||:||:||.:|||||||.|::|:|||||||:|||::||:||||||||:
Human   270 ARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGR 334

  Fly   327 PLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNI 391
            |||::|.||||||||:|||||||||||||||||||||||.|||||||||||.|:||.|:||||||
Human   335 PLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNI 399

  Fly   392 DRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTL 456
            ||:|||||||||||||||||:||||:|||||||||.||||||.|||||||:.|.|:|||||||||
Human   400 DRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTL 464

  Fly   457 HKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQ 521
            ||||||||||::|||||||:|||.|:||||||||||||||:|||:|||||::.:.:.||.|:|||
Human   465 HKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQ 529

  Fly   522 ANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQIFSL 586
            :|:|||||||||||||||.||||||:.|.:||||||||.|||.|:|||.||||||||||||||||
Human   530 SNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSL 594

  Fly   587 IIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHICFLE 651
            ||||::|.|||||||||:||.||||.||||||||:||:||||||||||||||||||||:||.:||
Human   595 IIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLE 659

  Fly   652 LGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAHNME 716
            :||||...||.|||||||||||.|||:|||||:|||.:||.:||..|||||.|||.||:||||.|
Human   660 MGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNE 724

  Fly   717 LEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACVG 781
            |||||||||||||||:|||||||||||||.||:|||:||||.|:|||||:|||.||||||||.||
Human   725 LEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVG 789

  Fly   782 QQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKT 846
            ||||||||||:||:.|||||||||||||||||||||||||||||:||:|||||||||||||||||
Human   790 QQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKT 854

  Fly   847 AETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFEKR 911
            ||||||||||||:||||||.||.|||||:.|::|||||||||.||.|||||:.|:|.|||:|||:
Human   855 AETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKK 919

  Fly   912 FKFDWSNERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSLRQIFPNGESKVVLPCNLQ 976
            |:||::|||.:::...:|::|::..::....|||.|::|:..||:.||.|||.|:|||||||||.
Human   920 FRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLL 984

  Fly   977 RMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLFQCLIRSTL 1041
            |||||.|||||||.|||:||.||:|::|||.|.::.|||.|:|.:|:||.|||||||...:||||
Human   985 RMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTL 1049

  Fly  1042 CTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAGVSS 1106
            |::.::||||||.|||:||:||||::|.||.|:||||||||||||||||||||||||||:||||:
Human  1050 CSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSA 1114

  Fly  1107 KNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIYYDP 1171
            ||||||||||||:||||||||.|||||||.|.:|||||:||::||||||||||||||||||||||
Human  1115 KNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDP 1179

  Fly  1172 DPQRTVISEDQEFVNVYYEMPDFDPTRISPWLLRIELDRKRMTDKKLTMEQIAEKINVGFGEDLN 1236
            :||.||::||||:|||||||||||..||||||||:|||||.|||:||||||||||||.|||:|||
Human  1180 NPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLN 1244

  Fly  1237 CIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQGIEAIGKVYM 1301
            ||||||||:||||||||||::|||.|:|:|.||||:||:||||||:|||:||||||||.|.||||
Human  1245 CIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYM 1309

  Fly  1302 HLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICEIFQVLGIEAV 1366
            ||||||:||:|:|||.|||||:.||:|||||.|:|:||||:||||:||:||||.|||.|||||||
Human  1310 HLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAV 1374

  Fly  1367 RKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVL 1431
            ||::|:|:..|:.|.|.||||||||||||.||.:||||||||||:||||||.||:||||||||||
Human  1375 RKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVL 1439

  Fly  1432 MDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIEIP-NTLGNSMLGGAAMFIG 1495
            |:||||.|:|||:||||||::|||...||||||||||||||::|:||| |..|....|...||.|
Human  1440 MEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFG 1504

  Fly  1496 GGSTP--SMTPPMTPWANCNTPRYFS-PPGHVSAMTPGGPSFSPSAASDASGMSPSWSPA---HP 1554
            ...:|  .::|.||||....||.|.: .|...|.||||...|||||||||||.||.:|||   .|
Human  1505 SAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTP 1569

  Fly  1555 GSSPSSPGPSMSPYFPASP--SVSPSYSPTSPNYTASSPGG-----------------ASPNYSP 1600
            | ||.||||| |||.| ||  ::|||||||||.|...||||                 .||:|||
Human  1570 G-SPGSPGPS-SPYIP-SPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSP 1631

  Fly  1601 SSPNYSPTSPLYA--SPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQS--SPSFAGSG 1661
            :|||||||||.|:  ||.|:.|:|:::|.|..|||:|..||||||.||||....|  |||::.:
Human  1632 TSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT- 1695

  Fly  1662 SNIYSPGN-AYSPSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTP 1725
            |..|||.: :|||:|.:|||.|||||||||||||:|||||||||.||||||:||||||||||.:|
Human  1696 SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSP 1760

  Fly  1726 SYSPTSPNYS-ASPQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGS------------PGSPQY 1777
            |||||||:|| .||.|:|.||.||.|...||||||:|||.||||..|            |.||.|
Human  1761 SYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSY 1825

  Fly  1778 TPGSPQYSPASPKYSPTSPLYSPSSPQHSP-SNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSP 1841
            :|.||.|||.||||:||||.||||||:::| |.:||||...||.|||:|||....|||::.||||
Human  1826 SPSSPSYSPTSPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSP 1890

  Fly  1842 TSPTYTPTARNYSPTSPMYSPTAPSHYSPTSPAYSPSSPTF 1882
            |||||:||:..|:||||.||||:|: ||||||.|||:|||:
Human  1891 TSPTYSPTSPVYTPTSPKYSPTSPT-YSPTSPKYSPTSPTY 1930

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpII215	NP_511124.1	PRK08566	14..887	CDD:236292	699/876 (80%)
		RNAP_II_RPB1_N	15..868	CDD:259848	685/856 (80%)
		RNA_pol_Rpb1_5	822..1420	CDD:282807	445/597 (75%)
		RNA_pol_Rpb1_6	890..1071	CDD:282801	105/180 (58%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	334/417 (80%)
		TCRP1	1602..1880	CDD:291605	188/296 (64%)
		RCR	<1789..1864	CDD:304939	49/75 (65%)
POLR2A	NP_000928.1	RNAP_II_RPB1_N	19..876	CDD:259848	685/856 (80%)
		Bridging helix	833..845		9/11 (82%)
		RNA_pol_Rpb1_6	896..1079	CDD:398590	106/182 (58%)
		RNAP_II_Rpb1_C	1058..1476	CDD:132720	334/417 (80%)
		CTD	1491..1610	CDD:215026	65/121 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1546..1970		241/390 (62%)
		C-terminal domain (CTD), 52 X 7 AA approximate tandem repeats of Y-[ST]-P-[STQ]-[ST]-P-[SRTEVKGN]	1593..1960		207/340 (61%)
		Herpes_BLLF1	<1854..>1959	CDD:282904	50/78 (64%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165158796
Domainoid	1	1.000	928	1.000	Domainoid score	I43
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H721
Inparanoid	1	1.050	2828	1.000	Inparanoid score	I22
Isobase	1	0.950	-	0	Normalized mean entropy	S300
OMA	1	1.010	-	-		QHG53835
OrthoDB	1	1.010	-	-		D591636at2759
OrthoFinder	1	1.000	-	-		FOG0000819
OrthoInspector	1	1.000	-	-		oto91412
orthoMCL	1	0.900	-	-		OOG6_100210
Panther	1	1.100	-	-	LDO	PTHR19376
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1183
SonicParanoid	1	1.000	-	-		X2195
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	15	14.890