DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpII215 and polr1a

DIOPT Version :9

Sequence 1:	NP_511124.1	Gene:	RpII215 / 32100	FlyBaseID:	FBgn0003277	Length:	1887	Species:	Drosophila melanogaster
Sequence 2:	XP_001922839.2	Gene:	polr1a / 327078	ZFINID:	ZDB-GENE-030131-5286	Length:	1693	Species:	Danio rerio

Alignment Length:	1789	Identity:	474/1789 - (26%)
Similarity:	768/1789 - (42%)	Gaps:	437/1789 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 KAPLRQVKRVQFGILSPDEIRRMSV-TEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTCA 71
            :.|.|:::.:.||:.|.||.|::|| |....|..:.:  |.....||.|...|..|....|.||.
Zfish     6 ETPWRRLEGMSFGLFSADETRKLSVKTITNPQLLDNV--GNAAANGLYDLALGPADNKEICSTCM 68

  Fly    72 GNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRKR 136
            .:.:.||||||||:|..||::..|..|...::|..|..|.              ::|.   ||..
Zfish    69 QDFSSCPGHFGHIELPLPVYNPLFFDKLYLLIRGSCLSCH--------------ILKC---PRAA 116

  Fly   137 LAYVYDLCKGKTICEG-----------------------GEDMDLT------------KENQQPD 166
            :..:  ||:.|.:..|                       ||::..|            |:.|..|
Zfish   117 IHLL--LCQLKLLDVGAMKEVFEIENVLNQFLESNPSPPGEEIQETLNDFVKPILETRKDKQHTD 179

  Fly   167 P-----NKKPG--------HGG------CGHYQPSIRR-------TGLDLTAEWKHQNEDSQEKK 205
            |     .||..        |.|      |...:.::|:       ....|..:...|::|||..|
Zfish   180 PVKHICEKKSSLITSFWRIHMGPKKCQNCKANRMTVRKEHNSKLIVTQTLHKKASEQDDDSQLTK 244

  Fly   206 IV-----VSAE---RVWE----ILKHITDEECFILGMDPKYA----RPDWMIVTVLPVPPLAVRP 254
            .:     |:.|   ::||    .|||:      ..|.|...:    .|:...:.:|.|||...||
Zfish   245 RIFLTPSVAREHIIKLWETEGFFLKHL------FSGFDMDNSDNGFNPEVFFLELLVVPPCRYRP 303

  Fly   255 -------------AVVMFGAAKN----QDDLT----HKLSDIIKANNELRKNEASGAAAHVIQEN 298
                         .|.|....|:    |..||    .|...|.....||.::..:.....:.|..
Zfish   304 INRLGDQMFTNGQTVNMQAVLKDGLIIQKLLTLIADEKTKAIEGTQTELEQSSEAPKVTEIDQSF 368

  Fly   299 IKMLQ------------FHVATLV----DNDM--------PGMPRAMQKSGKPLKAIKARLKGKE 339
            :..:|            .|:.:||    |:||        ||:.:.::|              ||
Zfish   369 LTSIQGVTLTDKLYSTWIHLQSLVNIVFDSDMDKLITEKFPGVRQLLEK--------------KE 419

  Fly   340 GRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQ 404
            |..|.::||||||::||:||.||..:..:::|:|...|..||:|:.|||:|:..|::.|..|.:.
Zfish   420 GLFRKHMMGKRVDYAARSVICPDMYIGTNEIGIPMVFATKLTYPQPVTPWNVKEMRQAVLNGPNI 484

  Fly   405 YPGAKYIVRDNGERIDL-------------RFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTL 456
            :|||..:|.::|.|..|             :....|:..|......|.||:::.|:::.||||||
Zfish   485 HPGATMVVNEDGSRTILSSTNQTQREAVAKQLLTPSTGQHRIPMKIVNRHIKNGDVLLLNRQPTL 549

  Fly   457 HKMSMMGHRVKVLPW-STFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITP 520
            |:.|:..|..::||. ...|::.:....|||||||||||.|.|||..:|||...:..|.:|.:.|
Zfish   550 HRPSIQAHCARILPGEKVLRLHYANCKAYNADFDGDEMNAHFPQSELSRAEAYTLVSTDQQYLVP 614

  Fly   521 QANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWD----AKMPQPCILKPRPLWTGK 581
            :..||:.|::||.:.:...||.|..|.||:| ...|::....|    .|:..|.::||..|||||
Zfish   615 KDGKPLAGLIQDHMVSGTSMTIRGCFFTRDQ-YTALVYRGLTDKIGRVKLLPPTLIKPVLLWTGK 678

  Fly   582 QIFSLIIPGNVNMIRTHSTHPDEEDEG--PYK-WIS------PG-------DTKVMVEHGELIMG 630
            |:.|.::   :|:|..:|...:...:.  |.| |:.      ||       :::|::..|||:||
Zfish   679 QVVSTLL---LNVIPENSIPLNLTGKAKIPGKAWVKESPRPVPGYVPESMCESQVVIRQGELLMG 740

  Fly   631 ILCKKSLGTSAGSLLHICFLELGHDIAGRFYGNIQTVINNWL-LFEGHSIGIGDTIADPQTYNEI 694
            :|.|...|:||..|:|.|:...|.:.:|:....:..:...:| |:.|.::|:.|.:.......:.
Zfish   741 VLDKAHYGSSAYGLVHCCYELYGGETSGKLLSCLARLFTAYLQLYRGFTLGVEDILVKDGANKQR 805

  Fly   695 QQAIKKAKDDVINVIQKAHN------------------MELEPTPGNTLRQTFENKVNRILNDAR 741
            ::.|||:.......:|.|.|                  :.::....|.:...|:.:||::.|:..
Zfish   806 KKIIKKSVTCGSKALQAAFNLPASAEDTEARERWQDAHLNIDQRDFNMVDLKFKEEVNQVNNNIN 870

  Fly   742 D---KTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACVGQQNVEGKRIPYGFRKRTLPHFI 803
            .   ..|  ..:|..| |||:.||.||:|||.:|..|:...:||..:||:|.|.....::||.|.
Zfish   871 KVCMPLG--LHRSFPE-NNLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMPSGKSLPCFQ 932

  Fly   804 KDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKTAETGYIQRRLIKAMESVMVNYD 868
            ..:..|.|.|||...:|.|:.|.||:||.|.|||||:||||||:.:||:||.:||.:|.::|.||
Zfish   933 PYEPQPRSGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCVIKHLEGLVVQYD 997

  Fly   869 GTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFEKRFKFDWSN-ERLMKKVFTDDVIK 932
            .|||:|.|.::|..||||||        ::|..:...   .::|.|...| |.:.|....|:|:.
Zfish   998 LTVRDSDGSVVQFLYGEDGL--------DIPKTQFLQ---PRQFPFIKDNYEVIRKSKCLDEVLA 1051

  Fly   933 EM-----TDSSEAIQELEAEWDRLVSDRDSLRQIFPNGESKVVLPCNLQRMIWNVQKIFHINKRL 992
            :|     .:..:||::.:|:.:. .|.||....:|...:        :.::...|:....:|.|
Zfish  1052 KMDPVAALNHFKAIKKWKAKHEH-ASPRDGAFLLFSQKK--------MSKLKAQVEGHELVNGR- 1106

  Fly   993 PTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANE----------------NATLLFQCLIRSTL 1041
              :.:.:::|:..:.|.|     :|..|.||:.:.                :.:..|..::.|.|
Zfish  1107 --EEATLQLIERWRALDE-----SGRMRYSKKTSHCPEPCLGLYRPDISFGSVSETFDSIVESYL 1164

  Fly  1042 CTKYVSEEFRLSTEA--FEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAGV 1104
            ....:.:||..|:|.  .:.|...::.::|:|..:|||.||.:||||:|||:||||||||||||.
Zfish  1165 NQFNIKQEFSASSETTDADRLRNLLQLKWQRALCDPGEAVGLVAAQSIGEPSTQMTLNTFHFAGR 1229

  Fly  1105 SSKNVTLGVPRLKEIINI-SKKPKAP--SLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTA 1166
            ...|||||:|||:||:.: |...|.|  |:.|.:...|.:..:           |..:|:|
Zfish  1230 GEMNVTLGIPRLREILLVASSNIKTPMMSIPVVMNKKALKQVK-----------TLRKKIT---- 1279

  Fly  1167 IYYDPDPQRTVISEDQEFVNVY--YEMPDFDPTRISPWLL--------RIELDRKRMTD------ 1215
                    |..:||....|||.  :.||..:..:...:.:        |.:.|:....|
Zfish  1280 --------RVCLSEVLHKVNVIESFRMPKKETLKSRVFKITFHFLPPERYQQDKLLNPDEILHYM 1336

  Fly  1216 ------------KKLTMEQIAEKINVGFGEDLNCIFND-------------DNADKLVLRIRIMN 1255
                        ||||.:..|    :...|......||             ||..:.....||::
Zfish  1337 EQTFFRMLLEAIKKLTAKLAA----ISLVETRRATVNDNDRDTSDGGADREDNGGEDAENERIVD 1397

  Fly  1256 NEENKFQDEDEAVD-----KMEDDMFLRCIEANMLSDM--------------------------- 1288
            :|.|  :.:.:|.|     |.|:::.....|....||.
Zfish  1398 DEAN--EGDADATDSKRKEKQEEEVDYESEEGEEGSDQEQEEVAEEQEASQEVSEEGSTESQQRV 1460

  Fly  1289 -------------------------------TLQG-------IEAIGKVYMHLP----------- 1304
                                           |.:|       :..|.||:..|.
Zfish  1461 NSEQPKGSSQGSVRINSVLQLSAAIEDYKYDTKKGLWCELTLVMPITKVHFDLTSVVVKQAQNAV 1525

  Fly  1305 --QTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDV-DPIRTSSNDICEIFQVLGIEAV 1366
              :|....|.::.|........|.:|.|:|.::.::....|: |..|..||::..:....|||..
Zfish  1526 IMETKGITRCLLNEVTRKDGSKEIMLNTEGINIHEMFKYADILDLKRLYSNEVHAMASTYGIEVA 1590

  Fly  1367 RKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETVDVL 1431
            .:.:|||:..|...||:.|:.|||:|:.|.|..:|....:.|.|| :.::..|.:.:||.:...|
Zfish  1591 LRVIEKEIKDVFAVYGIEVDPRHLSLVADYMCFEGVYKPLNRFGI-QSNSSPLQQMTFETSFKFL 1654

  Fly  1432 MDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDL 1465
            ..|......|.:...|..:::|::.|.|||.|||
Zfish  1655 KQATMLGSYDKLSSPSACLVVGKVVKGGTGIFDL 1688

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpII215	NP_511124.1	PRK08566	14..887	CDD:236292	305/1051 (29%)
		RNAP_II_RPB1_N	15..868	CDD:259848	294/1031 (29%)
		RNA_pol_Rpb1_5	822..1420	CDD:282807	189/749 (25%)
		RNA_pol_Rpb1_6	890..1071	CDD:282801	35/204 (17%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	134/546 (25%)
		TCRP1	1602..1880	CDD:291605
		RCR	<1789..1864	CDD:304939
polr1a	XP_001922839.2	RNA_pol_rpoA1	10..1024	CDD:274106	310/1069 (29%)
		RNAP_I_RPA1_N	16..997	CDD:259844	294/1028 (29%)
		RNA_pol_Rpb1_5	951..1644	CDD:282807	190/750 (25%)
		RNAP_I_Rpa1_C	1192..1689	CDD:132722	130/527 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D591636at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.920