DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpII215 and rpb1

DIOPT Version :9

Sequence 1:	NP_511124.1	Gene:	RpII215 / 32100	FlyBaseID:	FBgn0003277	Length:	1887	Species:	Drosophila melanogaster
Sequence 2:	NP_595673.1	Gene:	rpb1 / 2540292	PomBaseID:	SPBC28F2.12	Length:	1752	Species:	Schizosaccharomyces pombe

Alignment Length:	1899	Identity:	1007/1899 - (53%)
Similarity:	1303/1899 - (68%)	Gaps:	179/1899 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 SKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETME--GGRPKLGGLMDPRQGVIDRTSRCQT 69
            |..|||:|:.||||||||:|||.|||.:  ::|.|||:  |.||::|||:|||.|.|||..:|||
pombe     9 SSVPLRRVEEVQFGILSPEEIRSMSVAK--IEFPETMDESGQRPRVGGLLDPRLGTIDRQFKCQT 71

  Fly    70 CAGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPR 134
            |...|.:||||||||:||||||||||::|..|||.|||:.|.|:.:...|||..: ..:.| .|:
pombe    72 CGETMADCPGHFGHIELAKPVFHIGFLSKIKKILECVCWNCGKLKIDSSNPKFND-TQRYR-DPK 134

  Fly   135 KRLAYVYDLCKGKTICE-----GGEDMDLTKENQQPDPNKKPGHGGCGHYQPSIRRTGLDLTAEW 194
            .||..|:::||.|.:|:     |.::.||:      :|:...||||||..||:||:.||.|...|
pombe   135 NRLNAVWNVCKTKMVCDTGLSAGSDNFDLS------NPSANMGHGGCGAAQPTIRKDGLRLWGSW 193

  Fly   195 KHQNEDSQ--EKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVV 257
            |...::|.  ||:::...| |..|..||:.|:...||::.:||||||||:|||||||.:|||::.
pombe   194 KRGKDESDLPEKRLLSPLE-VHTIFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSIS 257

  Fly   258 MFGAAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQ 322
            :.|.::.:||||||||||||||..:|:.|..||.||::.|..::|||||||.:||::.|.|:|:|
pombe   258 VDGTSRGEDDLTHKLSDIIKANANVRRCEQEGAPAHIVSEYEQLLQFHVATYMDNEIAGQPQALQ 322

  Fly   323 KSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVT 387
            |||:|||:|:|||||||||:||||||||||||||||||.||||.:|::|||||||:.||:||.||
pombe   323 KSGRPLKSIRARLKGKEGRLRGNLMGKRVDFSARTVITGDPNLSLDELGVPRSIAKTLTYPETVT 387

  Fly   388 PFNIDRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNR 452
            |:||.::|||||.|..::||||||:||.|||||||:|.::.|:.|:.|::||||:||.|:|||||
pombe   388 PYNIYQLQELVRNGPDEHPGAKYIIRDTGERIDLRYHKRAGDIPLRYGWRVERHIRDGDVVIFNR 452

  Fly   453 QPTLHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQI 517
            ||:||||||||||::|:|:||||:|||.||||||||||||||:|||||.|||||::.|.:.|:||
pombe   453 QPSLHKMSMMGHRIRVMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSEETRAEIQEITMVPKQI 517

  Fly   518 ITPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQ 582
            ::||:||||||||||||..|||.:.||.|:||..|||:::::|.||..:|.|.||||:.||||||
pombe   518 VSPQSNKPVMGIVQDTLAGVRKFSLRDNFLTRNAVMNIMLWVPDWDGILPPPVILKPKVLWTGKQ 582

  Fly   583 IFSLIIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHI 647
            |.|||||..:|:||         |:......:|.|:.:::|:||:|.|::.||::|.|.|.|:|.
pombe   583 ILSLIIPKGINLIR---------DDDKQSLSNPTDSGMLIENGEIIYGVVDKKTVGASQGGLVHT 638

  Fly   648 CFLELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKA 712
            .:.|.|.:|...|:..||.|:|.|||..|.||||||||||..|..|:.:.:|:|:..|...||.|
pombe   639 IWKEKGPEICKGFFNGIQRVVNYWLLHNGFSIGIGDTIADADTMKEVTRTVKEARRQVAECIQDA 703

  Fly   713 HNMELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVI 777
            .:..|:|.||.|||::||.||:||||.|||..|.||:.||.:.||:|.||.:|||||.|||||:.
pombe   704 QHNRLKPEPGMTLRESFEAKVSRILNQARDNAGRSAEHSLKDSNNVKQMVAAGSKGSFINISQMS 768

  Fly   778 ACVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDT 842
            ||||||.|||||||:||:.||||||.|||..||||||:|||||.||||.||:||||.||||||||
pombe   769 ACVGQQIVEGKRIPFGFKYRTLPHFPKDDDSPESRGFIENSYLRGLTPQEFFFHAMAGREGLIDT 833

  Fly   843 AVKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKS 907
            ||||||||||||||:||||.|||.|||||||::|.:||..||||||...|||:|...:::||.|.
pombe   834 AVKTAETGYIQRRLVKAMEDVMVRYDGTVRNAMGDIIQFAYGEDGLDATLVEYQVFDSLRLSTKQ 898

  Fly   908 FEKRFKFDWSNERLMKKVFTDDVIKEMTDSSEAIQE-LEAEWDRLVSDRDSL-RQIFPNGESKVV 970
            |||:::.|...:|.:.....:.:     ::..::|: |:.|:.:||:||:.| :.|||.|:::..
pombe   899 FEKKYRIDLMEDRSLSLYMENSI-----ENDSSVQDLLDEEYTQLVADRELLCKFIFPKGDARWP 958

  Fly   971 LPCNLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLFQC 1035
            ||.|:||:|.|..:|||:..:.||||.|..:|.|:..|:.:..|..|:|||::....|||||||.
pombe   959 LPVNVQRIIQNALQIFHLEAKKPTDLLPSDIINGLNELIAKLTIFRGSDRITRDVQNNATLLFQI 1023

  Fly  1036 LIRSTLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFH 1100
            |:||....|.|..|:||:..||||::||:|.|||||..:||||||.|||||:|||||||||||||
pombe  1024 LLRSKFAVKRVIMEYRLNKVAFEWIMGEVEARFQQAVVSPGEMVGTLAAQSIGEPATQMTLNTFH 1088

  Fly  1101 FAGVSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANT 1165
            :||||||||||||||||||:|::|..|.||||::|....|.:.:.||||..::|||||..||:.|
pombe  1089 YAGVSSKNVTLGVPRLKEILNVAKNIKTPSLTIYLMPWIAANMDLAKNVQTQIEHTTLSTVTSAT 1153

  Fly  1166 AIYYDPDPQRTVISEDQEFVNVYYEMPDFDPT----RISPWLLRIELDRKRMTDKKLTMEQIAEK 1226
            .|:||||||.|||.||::||..::.:||.:..    :.||||||:||||.:|.||||:|..:|.|
pombe  1154 EIHYDPDPQDTVIEEDKDFVEAFFAIPDEEVEENLYKQSPWLLRLELDRAKMLDKKLSMSDVAGK 1218

  Fly  1227 INVGFGEDLNCIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQ 1291
            |...|..||..|:::||||||::|.||:.:::.|.:|:|..:   |:|:||:.||.:||..::|:
pombe  1219 IAESFERDLFTIWSEDNADKLIIRCRIIRDDDRKAEDDDNMI---EEDVFLKTIEGHMLESISLR 1280

  Fly  1292 GIEAIGKVYMHLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICE 1356
            |:..|.:|||.     ..|.:...|.|.|:...||:|||||.::.:.::...||..||.||...|
pombe  1281 GVPNITRVYMM-----EHKIVRQIEDGTFERADEWVLETDGINLTEAMTVEGVDATRTYSNSFVE 1340

  Fly  1357 IFQVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMR 1421
            |.|:|||||.|.::.||:..|::|.|.|||||||||||||||::|||||||||||||.:||||||
pombe  1341 ILQILGIEATRSALLKELRNVIEFDGSYVNYRHLALLCDVMTSRGHLMAITRHGINRAETGALMR 1405

  Fly  1422 CSFEETVDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAE---KCRFGIEIPNTLG 1483
            |||||||::||||||..|.|..:|:||||::|||..||||.||:.||.:   ....|..:|...|
pombe  1406 CSFEETVEILMDAAASGEKDDCKGISENIMLGQLAPMGTGAFDIYLDQDMLMNYSLGTAVPTLAG 1470

  Fly  1484 NSMLGGAAMFIGGGSTPSMTPPMTPWANCNTPRYFSPPGHVSAMTPGGPSFSP-----SAASDAS 1543
            :.|  |.:....|..||....||.            ..|.|.  :|...:|||     |...:..
pombe  1471 SGM--GTSQLPEGAGTPYERSPMV------------DSGFVG--SPDAAAFSPLVQGGSEGREGF 1519

  Fly  1544 GMSPSWSPAHPGSSPSSPGPSMSPYFPASPSVSPSYSPTSPNYTASSPG-GASPNYSPSSPNYSP 1607
            |.......|.|.....|||.: ||:   |.::||.|..|||:|:.|||| ..||.|.||||:|||
pombe  1520 GDYGLLGAASPYKGVQSPGYT-SPF---SSAMSPGYGLTSPSYSPSSPGYSTSPAYMPSSPSYSP 1580

  Fly  1608 TSPLYASPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQSSPSFAGSGSNIYSPGNAYS 1672
            |||                          .||||||.||||     |||              ||
pombe  1581 TSP--------------------------SYSPTSPSYSPT-----SPS--------------YS 1600

  Fly  1673 PSSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTPSYSPTSPNYSAS 1737
            |:|.:||..|||||||||||||:|||||||||.|||||||||||||:|:|.:||||||||:
pombe  1601 PTSPSYSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS---- 1661

  Fly  1738 PQYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGSPGSPQYTPGSPQYSPASPKYSPTSPLYSPSS 1802
                            ||||||:|||.||||  ||.||.|:|.||.|||.||.||||||.|||:|
pombe  1662 ----------------YSPTSPSYSPTSPSY--SPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTS 1708

  Fly  1803 PQHSPSNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSPTSPTYTPTARNYSPTSPMYSPTAPSH 1867
            |                                  .||||||:|:||:.:||||||.||||:||
pombe  1709 P----------------------------------SYSPTSPSYSPTSPSYSPTSPSYSPTSPS- 1738

  Fly  1868 YSPTSPAYSPSSPT 1881
            ||||||:|||:||:
pombe  1739 YSPTSPSYSPTSPS 1752

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpII215	NP_511124.1	PRK08566	14..887	CDD:236292	519/881 (59%)
		RNAP_II_RPB1_N	15..868	CDD:259848	505/861 (59%)
		RNA_pol_Rpb1_5	822..1420	CDD:282807	319/603 (53%)
		RNA_pol_Rpb1_6	890..1071	CDD:282801	72/182 (40%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	236/421 (56%)
		TCRP1	1602..1880	CDD:291605	139/277 (50%)
		RCR	<1789..1864	CDD:304939	30/74 (41%)
rpb1	NP_595673.1	RNAP_II_RPB1_N	18..859	CDD:259848	505/860 (59%)
		RNA_pol_Rpb1_6	879..1059	CDD:309914	73/184 (40%)
		RNAP_II_Rpb1_C	1038..1452	CDD:132720	236/421 (56%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	621	1.000	Domainoid score	I25
eggNOG	1	0.900	-	-		E1_COG0086
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H721
Inparanoid	1	1.050	1831	1.000	Inparanoid score	I5
OMA	1	1.010	-	-		QHG53835
OrthoFinder	1	1.000	-	-		FOG0000819
OrthoInspector	1	1.000	-	-		oto101974
orthoMCL	1	0.900	-	-		OOG6_100210
Panther	1	1.100	-	-	LDO	PTHR19376
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1183
SonicParanoid	1	1.000	-	-		X2195
TreeFam	1	0.960	-	-
	14	13.860