DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpII215 and Polr3a

DIOPT Version :9

Sequence 1:	NP_511124.1	Gene:	RpII215 / 32100	FlyBaseID:	FBgn0003277	Length:	1887	Species:	Drosophila melanogaster
Sequence 2:	NP_001074716.1	Gene:	Polr3a / 218832	MGIID:	2681836	Length:	1390	Species:	Mus musculus

Alignment Length:	1543	Identity:	508/1543 - (32%)
Similarity:	775/1543 - (50%)	Gaps:	270/1543 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 RQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGR-PKLGGLMDPRQGVIDRTSRCQTCAGNMT 75
            :::..:.||:.||:|:|:.:..:...:...:.:... |.|.|::|.|.|..::...|:||..|:.
Mouse    13 KKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNNHAPLLYGVLDHRMGTSEKDRPCETCGKNLA 77

  Fly    76 ECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPK-----IKE---IVMKSRGQ 132
            :|.||:|:|||..|.||:|:....|.||:.:|..|..:::|....:     :|.   ..::.||.
Mouse    78 DCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKQQFLDFLKRPGLTYLQKRGL 142

  Fly   133 PRKRLAYVYDLCKGKTICEGGEDMDLTKENQQPDPNKKPGHGGCGHYQPSIRRTGLDLTAEWKHQ 197
            .:|    :.|.|:.|:.|.                       .||.:..::::.||......|::
Mouse   143 KKK----ISDKCRKKSTCH-----------------------YCGAFNGTVKKCGLLKIIHEKYK 180

  Fly   198 NEDSQEKKIV------------VSAER-------------------VWEILKHITDEECFILGMD 231
            .    .||:|            .:.|.                   |..:.|.|..|:..:|.|:
Mouse   181 T----NKKVVDPIVSNFLQSFETAIEHNKEVEPLLGRAQENLNPLVVLNLFKRIPAEDVPLLLMN 241

  Fly   232 PKYARPDWMIVTVLPVPPLAVRPAVVM-FGAAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVI 295
            |:..:|..:|:|.|.||||.:||:||. ..:..|:||||.||::||..|:.::|:..|||...:|
Mouse   242 PESGKPSDLILTRLLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEIIFLNDVIKKHRISGAKTQMI 306

  Fly   296 QENIKMLQFHVATLVDNDMPGMPRAMQKSGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVIT 360
            .|:...||...|..:::::.|:|..|... |..:....|||||:||.||||.|||||||.||||:
Mouse   307 MEDWDFLQLQCALYINSELSGIPLNMAPK-KWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVIS 370

  Fly   361 PDPNLRIDQVGVPRSIAQNLTFPELVTPFNIDRMQELVRRGNSQYPGAKYIVRDN---------G 416
            ||||||||:|.||..:|:.|||||.|...||:.:::|||.|...:|||.:|.:.:         |
Mouse   371 PDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVRNGPDVHPGANFIQQRHMQMKRFLKYG 435

  Fly   417 ERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQPTLHKMSMMGHRVKVLPWSTFRMNLSCT 481
            .|..:....|..|:       |||||.|.|:|:|||||:|||:|:|.|..:|.|..|||.|....
Mouse   436 NREKMAQELKFGDI-------VERHLIDGDVVLFNRQPSLHKLSIMAHLARVKPHRTFRFNECVC 493

  Fly   482 SPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQIITPQANKPVMGIVQDTLTAVRKMTKRDVF 546
            :||||||||||||||:||:.|.:||...:..|...::||:..:|::..:||.||....:|.:|.|
Mouse   494 TPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTF 558

  Fly   547 ITREQVMNLL-MFLPTWDAK----MPQPCILKPRPLWTGKQIFSLII-PGNVNMIRTHSTHPDEE 605
            ..|.:...:: ..|...|.|    :|.|.||||..|||||||||:|: |.:.|.:|.:.....::
Mouse   559 FDRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANLRTKGKQ 623

  Fly   606 DEGPYKWISPGDTKVMVEHGELIMGILCKKSLGT-SAGSLLHICFLELGHDIAGRFYGNIQTVIN 669
            ..|..:.:...|:.|.:::.||:.|.:.|.:||: |..::.:|...:.|.:.|......:..:..
Mouse   624 YCGKGEDLCVNDSYVTIQNSELMCGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAP 688

  Fly   670 NWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINV--------IQKAHNMELEPTPGNTLR 726
            .:|...|.||||||....        |.:.|||.:::|.        |:..:..:|:..||.|..
Mouse   689 VYLSNRGFSIGIGDVTPG--------QGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAE 745

  Fly   727 QTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIACVGQQNVEGKRIP 791
            :|.|..:.:.|:..||..|.:..:.|.:.|:...|.:.|||||.|||||:|||||||.:.|.|:|
Mouse   746 ETLEALILKELSVIRDHAGSACLRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVP 810

  Fly   792 YGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTAVKTAETGYIQRRL 856
            .||..|:||||.|....|.::|||.||:.:||||:||:||.|.|||||:|||||||||||:||||
Mouse   811 DGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRL 875

  Fly   857 IKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSFEKRFKFDWSNERL 921
            :|::|.:...||.|||:|.|.:||..||.|||....:|.::.|.          .||....|.:.
Mouse   876 VKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAMEGKDEPL----------EFKRVLDNIKA 930

  Fly   922 MKKVFTDDVI--KEMTDSSEAIQELEAEWDRLVSDRDSLRQIFPNGESKVVLPCNLQRMIWNVQK 984
            :....|:..:  .::|.::|||.:.    :..:..:||.                ||.:     |
Mouse   931 VYPCQTEPALSKNDLTLTAEAIMKK----NEFLCCQDSF----------------LQEI-----K 970

  Fly   985 IFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLFQCLIRSTLCTKYVSEE 1049
            .|               ||||...:::       .|.....|:|.|...:.|.:....|....|:
Mouse   971 TF---------------IKGVSEKIKK-------TRDKYGINDNGTTEPRVLYQLDRITPTQIEK 1013

  Fly  1050 FRLSTEAFEWLVGEIET---RFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAGVSSKNVTL 1111
            |             :||   ::.:||..||..||||.|||:|||.|||||.|||||||:|.|:||
Mouse  1014 F-------------LETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITL 1065

  Fly  1112 GVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIYYDPDPQRT 1176
            ||||:|||||.||....|.:|..|  ....||:.|:.|..|:|                    :|
Mouse  1066 GVPRIKEIINASKAISTPIITAQL--DKDDDADYARLVKGRIE--------------------KT 1108

  Fly  1177 VISEDQEFVNVYYEMPD--FDPTRISPWLLRIELDRKRMTDKKLTMEQI-----AEKINVG---- 1230
            ::.|..|::...: :||  |       .|:::.|:|.|:...::..|.:     ..|:.|.
Mouse  1109 LLGEISEYIEEVF-LPDDCF-------ILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDV 1165

  Fly  1231 --FGEDLNCIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQGI 1293
              .||.:.|:...:|:...:..:.       :|..||                   |..:.:|||
Mouse  1166 AVHGEAVVCVTPRENSKSSMYYVL-------QFLKED-------------------LPKVVVQGI 1204

  Fly  1294 EAIGKVYMHLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICEIF 1358
            ..:.:..:|:.:...|::        ||      |..:|.::..|::...|...||:||:..|:.
Mouse  1205 PEVSRAVIHIDEQSGKEK--------FK------LLVEGDNLRAVMATHGVKGTRTTSNNTYEVE 1255

  Fly  1359 QVLGIEAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCS 1423
            :.|||||.|.::..|:...:..:|:.::.||:.||.|:||.||.::.|||.|:.:.....||..|
Mouse  1256 KTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLAS 1320

  Fly  1424 FEETVDVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEK 1471
            ||:|.|.|.|||...:.|.:.||||.||||....:|||.|.||..|.:
Mouse  1321 FEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKANR 1368

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpII215	NP_511124.1	PRK08566	14..887	CDD:236292	340/937 (36%)
		RNAP_II_RPB1_N	15..868	CDD:259848	329/917 (36%)
		RNA_pol_Rpb1_5	822..1420	CDD:282807	183/615 (30%)
		RNA_pol_Rpb1_6	890..1071	CDD:282801	30/185 (16%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	137/433 (32%)
		TCRP1	1602..1880	CDD:291605
		RCR	<1789..1864	CDD:304939
Polr3a	NP_001074716.1	PRK08566	7..932	CDD:236292	348/975 (36%)
		RNAP_III_RPC1_N	24..891	CDD:259847	330/913 (36%)
		RNA_pol_Rpb1_5	841..1317	CDD:282807	183/615 (30%)
		RNAP_III_Rpc1_C	1021..1360	CDD:132723	131/408 (32%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0086
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D591636at2759
OrthoFinder	1	1.000	-	-		FOG0000819
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100210
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	6	5.720