DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpII215 and ama-1

DIOPT Version :9

Sequence 1:	NP_511124.1	Gene:	RpII215 / 32100	FlyBaseID:	FBgn0003277	Length:	1887	Species:	Drosophila melanogaster
Sequence 2:	NP_500523.4	Gene:	ama-1 / 177190	WormBaseID:	WBGene00000123	Length:	1856	Species:	Caenorhabditis elegans

Alignment Length:	1907	Identity:	1274/1907 - (66%)
Similarity:	1551/1907 - (81%)	Gaps:	89/1907 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 DSKAPLRQVKRVQFGILSPDEIRRMSVTEGGVQFAETMEGGRPKLGGLMDPRQGVIDRTSRCQTC 70
            |.:||||.|.|||||||.|:||:||||..  |:|.|..|.|:||||||||||||||||..||.||
Worm     7 DFQAPLRIVSRVQFGILGPEEIKRMSVAH--VEFPEVYENGKPKLGGLMDPRQGVIDRRGRCMTC 69

  Fly    71 AGNMTECPGHFGHIDLAKPVFHIGFITKTIKILRCVCFYCSKMLVSPHNPKIKEIVMKSRGQPRK 135
            |||:|:|||||||::||||||||||:|||:||||||||||.::|:....|::.||:.|:....:|
Worm    70 AGNLTDCPGHFGHLELAKPVFHIGFLTKTLKILRCVCFYCGRLLIDKSAPRVLEILKKTGTNSKK 134

  Fly   136 RLAYVYDLCKGKTICEGGEDMDLTKENQQPDPNKKPGH------GGCGHYQPSIRRTGLDLTAEW 194
            ||..:|||||.|::|||..:    ||...||....|.:      ||||.||||.||.|:|:.|||
Worm   135 RLTMIYDLCKAKSVCEGAAE----KEEGMPDDPDDPMNDGKKVAGGCGRYQPSYRRVGIDINAEW 195

  Fly   195 -KHQNEDSQEKKIVVSAERVWEILKHITDEECFILGMDPKYARPDWMIVTVLPVPPLAVRPAVVM 258
             |:.|||:||:||:::||||.|:.:.||||:..::||||::|||:|||.|||||||||||||||.
Worm   196 KKNVNEDTQERKIMLTAERVLEVFQQITDEDILVIGMDPQFARPEWMICTVLPVPPLAVRPAVVT 260

  Fly   259 FGAAKNQDDLTHKLSDIIKANNELRKNEASGAAAHVIQENIKMLQFHVATLVDNDMPGMPRAMQK 323
            ||:||||||||||||||||.|.:|::|||:||||||:.:::::|||||||||||.:||:|.|.||
Worm   261 FGSAKNQDDLTHKLSDIIKTNQQLQRNEANGAAAHVLTDDVRLLQFHVATLVDNCIPGLPTATQK 325

  Fly   324 SGKPLKAIKARLKGKEGRIRGNLMGKRVDFSARTVITPDPNLRIDQVGVPRSIAQNLTFPELVTP 388
            .|:|||:||.|||||||||||||||||||||||||||.||||.||.|||||:|||||||||:|||
Worm   326 GGRPLKSIKQRLKGKEGRIRGNLMGKRVDFSARTVITADPNLPIDTVGVPRTIAQNLTFPEIVTP 390

  Fly   389 FNIDRMQELVRRGNSQYPGAKYIVRDNGERIDLRFHPKSSDLHLQCGYKVERHLRDDDLVIFNRQ 453
            ||:|::||||.||::||||||||:|:||.|:|||:||:::|||||.||:||||::|.|:::||||
Worm   391 FNVDKLQELVNRGDTQYPGAKYIIRENGARVDLRYHPRAADLHLQPGYRVERHMKDGDIIVFNRQ 455

  Fly   454 PTLHKMSMMGHRVKVLPWSTFRMNLSCTSPYNADFDGDEMNLHVPQSMETRAEVENIHITPRQII 518
            ||||||||||||||:|||||||||||.||||||||||||||||:|||:|||||:|.|.:.|||:|
Worm   456 PTLHKMSMMGHRVKILPWSTFRMNLSVTSPYNADFDGDEMNLHLPQSLETRAEIEEIAMVPRQLI 520

  Fly   519 TPQANKPVMGIVQDTLTAVRKMTKRDVFITREQVMNLLMFLPTWDAKMPQPCILKPRPLWTGKQI 583
            ||||||||||||||||.|||.||||||||....:|:|||:|||||.|:|||.||||:|||||||:
Worm   521 TPQANKPVMGIVQDTLCAVRMMTKRDVFIDWPFMMDLLMYLPTWDGKVPQPAILKPKPLWTGKQV 585

  Fly   584 FSLIIPGNVNMIRTHSTHPDEEDEGPYKWISPGDTKVMVEHGELIMGILCKKSLGTSAGSLLHIC 648
            ||||||||||::||||||||.||.|||||||||||||::|||||:.||:|.|::|.|||:|||:.
Worm   586 FSLIIPGNVNVLRTHSTHPDSEDSGPYKWISPGDTKVIIEHGELLSGIVCSKTVGKSAGNLLHVV 650

  Fly   649 FLELGHDIAGRFYGNIQTVINNWLLFEGHSIGIGDTIADPQTYNEIQQAIKKAKDDVINVIQKAH 713
            .||||::||..||.:||||||.||:.|||:|||||||||..||.:||..|:|||.||::||:|||
Worm   651 TLELGYEIAANFYSHIQTVINAWLIREGHTIGIGDTIADQATYLDIQNTIRKAKQDVVDVIEKAH 715

  Fly   714 NMELEPTPGNTLRQTFENKVNRILNDARDKTGGSAKKSLTEYNNLKAMVVSGSKGSNINISQVIA 778
            |.:||||||||||||||||||:|||||||:||.||:|||:|:||.|:|||||||||.||||||||
Worm   716 NDDLEPTPGNTLRQTFENKVNQILNDARDRTGSSAQKSLSEFNNFKSMVVSGSKGSKINISQVIA 780

  Fly   779 CVGQQNVEGKRIPYGFRKRTLPHFIKDDYGPESRGFVENSYLAGLTPSEFYFHAMGGREGLIDTA 843
            |||||||||||||:|||.|||||||||||||||:||||||||||||||||:||||||||||||||
Worm   781 CVGQQNVEGKRIPFGFRHRTLPHFIKDDYGPESKGFVENSYLAGLTPSEFFFHAMGGREGLIDTA 845

  Fly   844 VKTAETGYIQRRLIKAMESVMVNYDGTVRNSVGQLIQLRYGEDGLCGELVEFQNMPTVKLSNKSF 908
            |||||||||||||||||||||||||||||||:.|::|||||||||.|..||.|||||:|.:|..|
Worm   846 VKTAETGYIQRRLIKAMESVMVNYDGTVRNSLAQMVQLRYGEDGLDGMWVENQNMPTMKPNNAVF 910

  Fly   909 EKRFKFDWSNERLMKKVFTDDVIKEMTDSSEAIQELEAEWDRLVSDRDSLRQIFPNGESKVVLPC 973
            |:.|:.|.::.:.::|.:::||::|:.:|.:.|..:|:||.:|..||..||:|||.|::|:||||
Worm   911 ERDFRMDLTDNKFLRKNYSEDVVREIQESEDGISLVESEWSQLEEDRRLLRKIFPRGDAKIVLPC 975

  Fly   974 NLQRMIWNVQKIFHINKRLPTDLSPIRVIKGVKTLLERCVIVTGNDRISKQANENATLLFQCLIR 1038
            ||||:|||.||||.::.|.|.:|||:.||.||:.|.::.:||:|||.|||||..|||||...|:|
Worm   976 NLQRLIWNAQKIFKVDLRKPVNLSPLHVISGVRELSKKLIIVSGNDEISKQAQYNATLLMNILLR 1040

  Fly  1039 STLCTKYVSEEFRLSTEAFEWLVGEIETRFQQAQANPGEMVGALAAQSLGEPATQMTLNTFHFAG 1103
            ||||||.:..:.:|::|||:||:||||:|||||.|.||||||||||||||||||||||||||:||
Worm  1041 STLCTKNMCTKSKLNSEAFDWLLGEIESRFQQAIAQPGEMVGALAAQSLGEPATQMTLNTFHYAG 1105

  Fly  1104 VSSKNVTLGVPRLKEIINISKKPKAPSLTVFLTGGAARDAEKAKNVLCRLEHTTLRKVTANTAIY 1168
            ||:|||||||||||||||:||..|.|||||||||.||:|.||||:|||:||||||:|||.|||||
Worm  1106 VSAKNVTLGVPRLKEIINVSKTLKTPSLTVFLTGAAAKDPEKAKDVLCKLEHTTLKKVTCNTAIY 1170

  Fly  1169 YDPDPQRTVISEDQEFVNVYYEMPDFDPTRISPWLLRIELDRKRMTDKKLTMEQIAEKINVGFGE 1233
            |||||:.|||:||:|:|:::|||||.|.:|.||||||||||||||.|||||||.||::|:.|||.
Worm  1171 YDPDPKNTVIAEDEEWVSIFYEMPDHDLSRTSPWLLRIELDRKRMVDKKLTMEMIADRIHGGFGN 1235

  Fly  1234 DLNCIFNDDNADKLVLRIRIMNNEENKFQDEDEAVDKMEDDMFLRCIEANMLSDMTLQGIEAIGK 1298
            |::.|:.||||:|||.|:||..  |:|.:.::|.|||||||:||||||||||||:|||||.||.|
Worm  1236 DVHTIYTDDNAEKLVFRLRIAG--EDKGEAQEEQVDKMEDDVFLRCIEANMLSDLTLQGIPAISK 1298

  Fly  1299 VYMHLPQTDSKKRIVITETGEFKAIGEWLLETDGTSMMKVLSERDVDPIRTSSNDICEIFQVLGI 1363
            |||:.|.||.||||:||..|.||::.:|:||||||::::|||||.:||:||:||||||||:||||
Worm  1299 VYMNQPNTDDKKRIIITPEGGFKSVADWILETDGTALLRVLSERQIDPVRTTSNDICEIFEVLGI 1363

  Fly  1364 EAVRKSVEKEMNAVLQFYGLYVNYRHLALLCDVMTAKGHLMAITRHGINRQDTGALMRCSFEETV 1428
            |||||::|:||:.|:.|.|.|||||||||||||||||||||||||||||||:.||||||||||||
Worm  1364 EAVRKAIEREMDNVISFDGSYVNYRHLALLCDVMTAKGHLMAITRHGINRQEVGALMRCSFEETV 1428

  Fly  1429 DVLMDAAAHAETDPMRGVSENIIMGQLPKMGTGCFDLLLDAEKCRFGIEIPNTLGNSMLGGA--A 1491
            |:||:||.|||.||::||||||::|||.:.|||||||:||.|||::|:|||.   |.::||.  .
Worm  1429 DILMEAAVHAEEDPVKGVSENIMLGQLARCGTGCFDLVLDVEKCKYGMEIPQ---NVVMGGGFYG 1490

  Fly  1492 MFIGGGSTPSMTPPMTPWANCNTPRY----FSPPGHVSAMTPGGPSFSPSAASDASGMSP----S 1548
            .|.|..|....:|..:||.:..||.|    :||.  ...|:||. .|||:..:| .|.||    .
Worm  1491 SFAGSPSNREFSPAHSPWNSGVTPTYAGAAWSPT--TGGMSPGA-GFSPAGNTD-GGASPFNEGG 1551

  Fly  1549 WSPAHPGSSPSSPGPSMSPYFPASPSVSPS-YSPTSPNYTASSPGGASPNYSPSSPNYSPTSPLY 1612
            ||||.||    .|..::||..|:...:||. |||:||.::.:     ||:|||:||:||||||..
Worm  1552 WSPASPG----DPLGALSPRTPSYGGMSPGVYSPSSPQFSMT-----SPHYSPTSPSYSPTSPAA 1607

  Fly  1613 ----ASPRYASTTPNFNPQSTGYSPSSSGYSPTSPVYSPTVQFQSSPSFAGSGSNIYSPGNAYSP 1673
                .||.|:.|:|:::|.|..|||:|..||||||.||||     |||              |||
Worm  1608 GQSPVSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT-----SPS--------------YSP 1653

  Fly  1674 SSSNYSPNSPSYSPTSPSYSPSSPSYSPTSPCYSPTSPSYSPTSPNYTPVTPSYSPTSPNYSASP 1738
            :|.:|||:||||||:|||||||||.||||||.||||||:||||||.|:|.:|:||||||:|.:..
Worm  1654 TSPSYSPSSPSYSPSSPSYSPSSPRYSPTSPTYSPTSPTYSPTSPTYSPTSPTYSPTSPSYESGG 1718

  Fly  1739 QYSPASPAYSQTGVKYSPTSPTYSPPSPSYDGSPGSPQYTPGSPQYSPASPKYSPTSPLYSPSSP 1803
            .|||:||       ||||:||||||.||||  ||.||||:|.||||||:||.|:|:||.|:|:||
Worm  1719 GYSPSSP-------KYSPSSPTYSPTSPSY--SPTSPQYSPTSPQYSPSSPTYTPSSPTYNPTSP 1774

  Fly  1804 QHSPSNQYSPTGSTYSATSPRYSPNMSIYSPSSTKYSPTSPTYTP-------TARNYSPTSPMYS 1861
            :...|.|||||..|||.|||.       |:|||.:||||||||||       |:..||||||.||
Worm  1775 RGFSSPQYSPTSPTYSPTSPS-------YTPSSPQYSPTSPTYTPSPSEQPGTSAQYSPTSPTYS 1832

  Fly  1862 PTAPSHYSPTSPAYSPSSPTFE 1883
            |::|: |||.||:|||||||::
Worm  1833 PSSPT-YSPASPSYSPSSPTYD 1853

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpII215	NP_511124.1	PRK08566	14..887	CDD:236292	649/879 (74%)
		RNAP_II_RPB1_N	15..868	CDD:259848	633/859 (74%)
		RNA_pol_Rpb1_5	822..1420	CDD:282807	415/597 (70%)
		RNA_pol_Rpb1_6	890..1071	CDD:282801	94/180 (52%)
		RNAP_II_Rpb1_C	1050..1468	CDD:132720	308/417 (74%)
		TCRP1	1602..1880	CDD:291605	170/288 (59%)
		RCR	<1789..1864	CDD:304939	45/81 (56%)
ama-1	NP_500523.4	PRK08566	7..893	CDD:236292	657/891 (74%)
		RNAP_II_RPB1_N	17..870	CDD:259848	633/858 (74%)
		RNA_pol_Rpb1_5	824..1420	CDD:282807	415/597 (70%)
		RNA_pol_Rpb1_6	890..1073	CDD:282801	95/182 (52%)
		RNAP_II_Rpb1_C	1053..1468	CDD:132720	308/416 (74%)
		CTD	1493..1584	CDD:215026	35/98 (36%)
		CytochromB561_N	<1735..>1828	CDD:286826	59/101 (58%)
		DUF3432	1746..1837	CDD:288743	56/97 (58%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160166310
Domainoid	1	1.000	854	1.000	Domainoid score	I20
eggNOG	1	0.900	-	-		E1_COG0086
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H721
Inparanoid	1	1.050	2561	1.000	Inparanoid score	I8
Isobase	1	0.950	-	0	Normalized mean entropy	S300
OMA	1	1.010	-	-		QHG53835
OrthoDB	1	1.010	-	-		D591636at2759
OrthoFinder	1	1.000	-	-		FOG0000819
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100210
Panther	1	1.100	-	-	LDO	PTHR19376
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1183
SonicParanoid	1	1.000	-	-		X2195
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	15	14.790