DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Myo10A and myo5b

DIOPT Version :9

Sequence 1:	NP_001036269.2	Gene:	Myo10A / 32028	FlyBaseID:	FBgn0263705	Length:	3145	Species:	Drosophila melanogaster
Sequence 2:	XP_021324561.1	Gene:	myo5b / 567402	ZFINID:	ZDB-GENE-031219-7	Length:	1840	Species:	Danio rerio

Alignment Length:	1759	Identity:	475/1759 - (27%)
Similarity:	711/1759 - (40%)	Gaps:	406/1759 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly   156 GVEDMTLLDDLHEASLLWNLRLRY-DKGLIYTFAGSILIAVNPYKMFPDAYGLEVAKQYAGRPLG 219
            |..|:|.|..|||.::|.||::|: :...|||:.|.:|:|:|||:.. ..||.||...|:|:.:|
Zfish    70 GENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQL-HIYGEEVINAYSGQNMG 133

  Fly   220 SLPPHLFAIGAAAHAAL---PSPQVVVISGESGSGKTESTKLVMQYLAAVVPGGGSASAV-ITEQ 280
            .:.||:||:...|...:   ...|.:::|||||:|||.|.|..|::.|.|   ||||:.. :.|:
Zfish   134 DMDPHIFAVAEEAFKQMARDEKNQSIIVSGESGAGKTVSAKYAMRFFATV---GGSANDTNVEEK 195

  Fly   281 ILEAAPLLEAFGNARTARNDNSSRFGKYLEVYF-KSGAIVGAKITQYLLEKSRIVTQAPGERNYH 344
            :|.::|::||.|||:|.|||||||||||:::.| :...|:||.:..|||||||:|.||..|||||
Zfish   196 VLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDRRYHIIGANMRTYLLEKSRVVFQAEEERNYH 260

  Fly   345 VFYELLGGLSETERSKYGLLEADKYFYLNQGATDCASGRVDWESL---QGAMQVLGVSEGEREGI 406
            :||:|....|..|.....|..|:.:.|.:.|......|..|.|.|   :.|:.:|||.|..:..|
Zfish   261 IFYQLCASCSLPEFKDLTLTSAEDFTYTSLGENIFIEGVNDAEDLVKTREALTMLGVKENHQMSI 325

  Fly   407 VRVLAAVLHLGNVYFHRRQLRHGQEGVEVGSDAEIKWAAHLLHISADGLHRALTSRTTEARAERL 471
            .:::|::||||||..  .|.|.|:......:|..:.....||.|..:.:...|..|.....:|..
Zfish   326 FKIIASILHLGNVEI--VQERDGESCHINRNDTHLHHFCRLLGIEQEQMENWLCRRKLVTTSETY 388

  Fly   472 HTPLGIDQALDARDAFAKALYAGLFNWLVSRINSIVQKGGTHDAHRISILDIFGFEDLAENSFEQ 536
            ...:...||::||||.||.:||.||:|:|..||..:.. .|.....|.:|||:|||....|||||
Zfish   389 VKNMPHSQAVNARDALAKHIYAHLFDWIVEHINKSLHT-STKQHSFIGVLDIYGFETFEINSFEQ 452

  Fly   537 LCINYANENLQLYFNKHVFKLEQAEYARERLEWTPLAWDDNLPVIHLLAKKPVGICHLLDDESNF 601
            .|||||||.||..||.|||||||.||.:|::.||.:.:.||.|.|.|:..| :||..|||:|...
Zfish   453 FCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAK-LGILDLLDEECKV 516

  Fly   602 PRATDLSFLEKCHYNHALSELYARPRIGAQEFGVTHYAGQVWYCVDGFLDKNRDALRGDVLELLA 666
            |:.||.::.:|.:..|:.|..:.:||:..:.|.|.|:|.:|.|..||||:||||.:..:.:.:|.
Zfish   517 PKGTDQNWAQKLYSKHSGSGHFEKPRMSNKSFIVVHFADKVEYQCDGFLEKNRDTVYEEQINILK 581

  Fly   667 SSRLPLVGEL------------TKQLRAQRDAGKTLPKGSNGRFVTMKPRTPTVAARFADSLQQL 719
            :|:..||.:|            :|..|......|..|||.|      :....||..:|.:||..|
Zfish   582 ASKFQLVADLFDDAKDAAPHPGSKTSRINVRPAKPAPKGHN------REHRKTVGTQFRNSLHLL 640

  Fly   720 LQSMGRCHPWFVRCIKPNQEKHALRMDMPCVLQQLRYLGMLDTIQIRQRGYPVRLRFQHFVERYR 784
            ::::....|.:|||||||..|.:...|....:||||..|:|:||:|...|||.|..:..|..|||
Zfish   641 METLNATTPHYVRCIKPNDYKESFVFDSRRAVQQLRACGVLETIRISAAGYPSRWTYPDFFSRYR 705

  Fly   785 HLLPSPLARGTPYRELCRALLEAMPRTGVEGPD-YQLGATRVFLREALHRALESGRTERLRRAAV 848
            .|:..........:::|:.|||.:    ::.|| :|.|.|::|.|......||..|.::.|.|.:
Zfish   706 VLMTKKDLSVGDKKQVCKNLLEIL----IKDPDKFQFGKTKIFFRAGQVAYLEKLRADKFRFACI 766

  Fly   849 SVQRHVRGMLVRRQ------------------LARRQAAATRL-------QARWRGQRAQQRYER 888
            .:|:.|||.|.|.:                  ||||.|...||       |.::|..:.::.|.|
Zfish   767 KIQKTVRGWLQRIRYRKIRKSAITLQRYGRGYLARRYAEMLRLTRAAVICQKQYRMVQVRREYLR 831

  Fly   889 LRKGALTAQRLWRGRQARRRVQQLRSDHR--------RRQEAREAAQRAREAREAKQA----VLE 941
            :|:..:|.|...||...||..|:....|:        |....|:..||:|.|....|.    |..
Zfish   832 VRRAVITIQAFTRGMFIRRLYQEFLLHHKAMIIQKTVRGWLVRKKYQRSRYAAIVIQCFYRRVRA 896

  Fly   942 RSQLSYLDIPAELAFIYSKLQGWSPPHGDRHLVKVLGTVPGPPSSAVQLPEDLGQFSFGKFSSVY 1006
            :.||..|.|.|..|..:.||.                  .|..:..|||.:.:...|
Zfish   897 KRQLKQLKIEARSAEHFKKLN------------------VGMENKIVQLQKKMDNQS-------- 935

  Fly  1007 CNGLRLQPRREPITA-----------------PLLTRAASRDQ-------------DFQDALAVF 1041
               ..|:.:.|.:..                 .|.||..:..|             :.|:|.|..
Zfish   936 ---KELKSQNENLAVAKTVLETEVSKLSKELETLRTRQVAGTQMTSLQEELEKLRAELQEAHAHK 997

  Fly  1042 KLI-LRWSNDK-ALEGAKEKLLADYIVHKALSSRGLRDEILVQLCNQVHGLPPNSGEATRLWQLL 1104
            ||: ..:||:| .||...|:|..:..|.|.......|   .:|...|..|...:..|:.
Zfish   998 KLLEEEFSNEKQGLEQRVEELEKENTVLKKEKEEMNR---RIQTSTQDQGGDVSQKESR------ 1053

  Fly  1105 GQCLCCFQPSAAFSKYLMRFVDDEAPESLRPLLLRQLLRQQGGGTSSGAVGAGACRSFVPAWLEW 1169
                            |...:|||......  |:::..|                       ||.
Zfish  1054 ----------------LQHELDDERQRYQN--LVKEYSR-----------------------LEQ 1077

  Fly  1170 RAWTRGCDMALPLTLPDEASQTVAVDSWTSCEEAAALAVSSLGVASRGWTLVLDDGQQLTDSCGL 1234
            |......||:.....|..........|..|.....::::|.:|..        ||..|..:..|:
Zfish  1078 RYENLQEDMSSMKFHPGHRRNPSNQSSLGSDSNYPSISLSEVGDT--------DDTIQQVEEMGI 1134

  Fly  1235 D-YVMDLIA--------------EKELCPAFPAPRSDLLRSGAKFARTTLPD---------AVKR 1275
            : ..|||..              .|.|............|.|::...:|..|         ::||
Zfish  1135 EKAAMDLSVFMKLQKRVRELEQERKRLQTNLDKVEELSKRKGSEAKNSTSEDDINADLAYNSLKR 1199

  Fly  1276 PAVPPPAPPTSSGKEDVPRERRSSRELLSRSSALNERYFEREPSPGPGQGSSTGQAKSRSKSLDD 1340
            ..:   .......|.|:...|::..:..|.:::.||..        .|......|.|:.::.||.
Zfish  1200 QEL---ETENKKLKNDLNELRKAVADRASENNSSNELQ--------DGYNLLLSQLKAANEELDV 1253

  Fly  1341 ------LLAGDIV---------------PVPTDCDSQEPLHTLGLSESRLNDRYHSAERLAPVGK 1384
                  :|...||               .|....|::.|::|    |.|::         ||..|
Zfish  1254 RKEEVLMLKTQIVNSTRQTENLNHINSNDVSEWTDNKSPVNT----EDRVD---------APASK 1305

  Fly  1385 DTGPRYQKSQHAGRRSHAASHG---SHSSKYADKAEYATRSSAMSDTSEAPSLASHVRRVRVPSQ 1446
            :      |:|..|..:.....|   ....:.|...|...::.......|..:|.:.:..::   .
Zfish  1306 N------KAQDWGYLNEDGELGLAYQGLKQVARLLETQLQTQTRQHRDELEALHTQIELLK---D 1361

  Fly  1447 ASDVDQFLDDLFSPVLDGSLDELSDARSLAASIRGGSYEQENEAESEIDDLDDYINDIFQPIPLV 1511
            ..:..|.:.|.||.:...:|.|.|                   .:.||..|.:...|:.:   ||
Zfish  1362 NLEKKQDMLDHFSTLSPDALVEFS-------------------VQQEITRLTNENLDLKE---LV 1404

  Fly  1512 QNLEKLTS--KDQLAAIIK-------GGGVASKPE----------ERDEESEIE----------- 1546
            :.|||...  |.||...:|       .....|:||          |:|.|..:|
Zfish  1405 EKLEKNEKKLKKQLRIYMKKVQELEVTQATKSRPELTRQFTVQRREKDFEGMLEYYKEDEALLVK 1469

  Fly  1547 ----DLD-----------------------DYINELFEPIPVAEGLDK-----LTSKEHLAVSIR 1579
                |:.                       ||||:           |:     ||:..:....:.
Zfish  1470 TLVTDMKPNAVSATVPCLPAYILFMCIRHADYIND-----------DQKVHSLLTATINAIKKVL 1523

  Fly  1580 GGGSTDSN-------GADPLLHQLMQLPGETESGPALYQQQVQRAFLQSAMAQNLQIQQQLLAQN 1637
            ...:.|..       .|..|||.|.|..|:                 ::.|.||...|.:...:|
Zfish  1524 KKNNDDFEMTSFWLANASRLLHCLKQYSGD-----------------EAFMTQNSAKQNEHCLKN 1571

  Fly  1638 -------QALQTLLSQ--QAAAAAANNSTSPPPAPPILSSLSISPPPPQSPMRMKATRSSLVVME 1693
                   |.|..|..|  |.....|..:..|.....:|.|.||.......||..: .|||.|..|
Zfish  1572 FDLAEYRQVLSDLSIQIYQQLVKVAEANMQPMIVSAMLESESIPSLAGVKPMGYR-NRSSSVDCE 1635

  Fly  1694 SLQP 1697
            |..|
Zfish  1636 SGGP 1639

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Myo10A	NP_001036269.2	MYSc	156..836	CDD:214580	268/701 (38%)
		MYSc_Myo15	169..828	CDD:276838	260/680 (38%)
		MyTH4	1014..1171	CDD:295320	32/188 (17%)
		MyTH4	2704..2810	CDD:279165
		B41	2823..3033	CDD:214604
		FERM_B-lobe	2920..3025	CDD:271216
		FERM_C_MyoXV	3029..3138	CDD:270022
myo5b	XP_021324561.1	Myosin_head	7..1371	CDD:332202	408/1438 (28%)
		MYSc_Myo5	83..746	CDD:276831	260/680 (38%)
		Myo5b_CBD	1461..1835	CDD:271261	41/208 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000014
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100101
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.810