Protein Alignment Sp1 and Klf4

DIOPT Version :10

• Sequence 1: NP_001259388.1
• Gene: Sp1 / 31913
• FlyBaseID: FBgn0020378
• Length: 726
• Species: Drosophila melanogaster
• Sequence 2: NP_034767.2
• Gene: Klf4 / 16600
• MGIID: 1342287
• Length: 483
• Species: Mus musculus
• Alignment Length: 568
• Identity: 132/568 - (23%)
• Similarity: 178/568 - (31%)
• Gaps: 248/568 - (43%)

  Fly    61 KSPPPLADAAVGKGFHP-------------------------W-------KKSPNSPA----AGS 89
            :.||..:|.||.....|                         |       |:.|..|.    ..:
Mouse     2 RQPPGESDMAVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWREELSHMKRLPPLPGRPYDLAA 66

  Fly    90 SGSSGGGGGGGGSSAGQHSPCAISAASSSSSSG--SSGGQSSRSLSSSASTMVNITASRPLASSC 152
            :.::....||.|::...::|..::...:...:.  ......|.||:...|....:|.|
Mouse    67 TVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTHQESVAATVTTS------- 124

  Fly   153 AAVGGGSTGSSSSASGSQSSSTASAVAAAY----GGDLYFPNTSTSNMDNHHMHQGLLGKVEAGA 213
                 .|..||||.:.|..:|..|..:.:|    |||   |..:.||...     |||
Mouse   125 -----ASASSSSSPASSGPASAPSTCSFSYPIRAGGD---PGVAASNTGG-----GLL------- 169

  Fly   214 AAFGGVYSRH---PYDWPFNAVTHKEAASVNSGWWDMHSAAGSWLDMGGAGMHSTMANYASENYS 275
                  |||.   |...|||      .|.:|              |:..:|      .:.:|...
Mouse   170 ------YSRESAPPPTAPFN------LADIN--------------DVSPSG------GFVAELLR 202

  Fly   276 SALSHSLLGSGQHLLQDTYKSMLPGQGVGVGVGVGMGGFSL---------PHSSPSAAAAAAATA 331
            ..|....:...|        ...||.|:       ||.|.|         .:||||..:.:.
Mouse   203 PELDPVYIPPQQ--------PQPPGGGL-------MGKFVLKASLTTPGSEYSSPSVISVSK--- 249

  Fly   332 AAAAGGSPQGGSPSTPSPRSQRRYAGRATCDCPNC-QEA-------------------------- 369
                 |||.|..|...:|     |:|.....||.. |||
Mouse   250 -----GSPDGSHPVVVAP-----YSGGPPRMCPKIKQEAVPSCTVSRSLEAHLSAGPQLSNGHRP 304

  Fly   370 ----------------------------------------------------------------- 369
                                                                             
Mouse   305 NTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLPPGFHPHPGPNYPPFLPDQMQSQVPSLHY 369

  Fly   370 ERLGPAGVHL--------------RKKN-IHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNW 419
            :.|.|.|..|              ||:. .|:|...||||.|.|:||||||||.||||:|:.|:|
Mouse   370 QELMPPGSCLPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDW 434

  Fly   420 LFCGKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLAKHVKTH 467
            ..||.:|.|||||.||.|.|||.:.|.|..|::.|.||||||.|:|.|
Mouse   435 DGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRH 482

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sp1	NP_001259388.1	SP6-9-like_N	50..386	CDD:411694	82/485 (17%)
		C2H2 Zn finger	390..409	CDD:275368	14/18 (78%)
		zf-H2C2_2	401..428	CDD:463886	17/26 (65%)
		C2H2 Zn finger	417..439	CDD:275368	13/21 (62%)
		zf-H2C2_2	431..454	CDD:463886	11/22 (50%)
		zf-C2H2	445..467	CDD:395048	12/21 (57%)
		C2H2 Zn finger	447..467	CDD:275368	11/19 (58%)
Klf4	NP_034767.2	KLF4_N	7..400	CDD:409228	80/479 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	22..42		0/19 (0%)
		9aaTAD. /evidence=ECO:0000250|UniProtKB:O43474	99..107		0/7 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	294..395		4/100 (4%)
		Interaction with ZNF296. /evidence=ECO:0000269|PubMed:24161396	386..483		52/97 (54%)
		COG5048	<395..478	CDD:227381	48/82 (59%)
		C2H2 Zn finger	402..424	CDD:275368	15/21 (71%)
		C2H2 Zn finger	432..454	CDD:275368	13/21 (62%)
		Interaction with target DNA	443..474		17/30 (57%)
		zf-H2C2_2	446..471	CDD:463886	12/24 (50%)
		C2H2 Zn finger	462..482	CDD:275368	11/19 (58%)