Protein Alignment Hecw and itcha

DIOPT Version :10

• Sequence 1: NP_572574.2
• Gene: Hecw / 31905
• FlyBaseID: FBgn0261931
• Length: 1426
• Species: Drosophila melanogaster
• Sequence 2: NP_001274024.1
• Gene: itcha / 100331274
• ZFINID: ZDB-GENE-120919-4
• Length: 851
• Species: Danio rerio
• Alignment Length: 1276
• Identity: 342/1276 - (26%)
• Similarity: 498/1276 - (39%)
• Gaps: 478/1276 - (37%)

  Fly   187 RKSWTLSPQSGGSTP-----LSGAKKKTK----TGAP------TTTLSNANGDGFNLWVARECFE 236
            :|:|.      |.:|     :.|..|||:    |.:|      |..::..:...|.:|    ..:
Zfish    18 KKNWF------GPSPYVEVFVDGQSKKTEKCTNTHSPKWKQSLTVIVTPFSKLIFRVW----SHQ 72

  Fly   237 TVPAEMVDTLSMAEVDEEVDEEEDEEAAAVESLLGAAAALQASAHVQRRQQQQHHQHLRQPSPPQ 301
            |:.|:::..::..||.|.:.....:....|::       ||.|:...|.:|
Zfish    73 TLKADILLGVASLEVSETLKANNMKICEVVQT-------LQLSSDRGRSEQ-------------- 116

  Fly   302 HRPLERSWPPRAVGRDLKLQGDVFQFDILDTDERLAEEMAQGSGSSNNSPLATPPVRFKPLLKKK 366
                        || ||.|..|..|   :|.:...:||....:..:..|.|....:|.:
Zfish   117 ------------VG-DLSLCLDGLQ---VDAETFASEEQKHNAVVNGESRLNGDAIRSR------ 159

  Fly   367 IGPDSYINTISTQKVPVHQSYEFPTFPSSSAVGSSSSSPNSLQFPYLRQHRPLTRALSNGKASSS 431
               ||..::..|...|:         |:..|||||:|:            .|.|.||
Zfish   160 ---DSSPSSDITDDAPL---------PNGHAVGSSASA------------SPSTGAL-------- 192

  Fly   432 EEQASPRLLLSPKRPRSPPSGCSTRSASPLDISLSPEQHSPTRGRGVGGALQDAVPPGTPTFHGQ 496
               .:||    |.||:.||..             ||.:|:.:.....|         .|||....
Zfish   193 ---RAPR----PLRPQRPPPA-------------SPHKHTSSTASSTG---------STPTHGSS 228

  Fly   497 RPQQRLLKRV---------GGGAGAGATGGATGSPLVCPPTPTHHARRHARLQAVTANSSLASGG 552
            .|...:..||         |..:|:|:......||  .||          |..|:| |..|
Zfish   229 APSPEMSPRVCVNGETESQGSSSGSGSNQAPRASP--TPP----------RAPAIT-NGPL---- 276

  Fly   553 AIPSDPPAVSSQLDYIYLQDGGQEQGQEQSDEVVHISSTRLPSIPERSAAAAAAAAAAAAGAALM 617
                 ||              |.||..:|:..|.::......:..||
Zfish   277 -----PP--------------GWEQRADQNGRVYYVDHIEKRTTWER------------------ 304

  Fly   618 EPGTGTVSGSSGAGAAGEPPLPPAWEARMDSHGRIFYIDHTTRTTSWQRPGAAPSGGPAGREQHH 682
                             ..||||.||.|:|..||::|:||..|||:||||
Zfish   305 -----------------PEPLPPGWERRLDPMGRVYYVDHINRTTTWQRP--------------- 337

  Fly   683 RQQLDRRYQSIRRTITNESRAAQLYNLPTSANSNANSTAPAAPPPATAIHPAILMLCRPDFYSLL 747
                  ..:|:|.....:::.:||
Zfish   338 ------TLESVRNYEEWQNQRSQL----------------------------------------- 355

  Fly   748 HTNEAALAIYNRNAALKHMVMRIRRDPPCFQRYQY--NKDLVALVNTFAQMSRELPSCWETKLDQ 810
               :.|:..:|                   ||:.|  .:.|....|........|||.||.:.|.
Zfish   356 ---QGAMQRFN-------------------QRFIYGLQEQLAPTANKEFDPLGPLPSGWEKRTDS 398

  Fly   811 SGKQFFIDHQHRRTSFMDPRLPVECPRVVRHRQQHSQQQSSVGYYYPDLVDGNCLSATSGHINAS 875
            :.:.:|:.|..|.|.:.|||                                     |.|.:|..
Zfish   399 NRRMYFVHHPTRSTQWEDPR-------------------------------------TQGLLNEK 426

  Fly   876 PAS-------SVSGVPPLPPPRPSSSSSSSRSSHGHGHNCNTTSAISCALNADRSAYGAAGMASA 933
            |..       :|.|:|                 :...||..||:.|.
Zfish   427 PLPEGWEMRFTVDGIP-----------------YFVDHNRRTTTYID------------------ 456

  Fly   934 GATAAGYEDVPIAYNEKVIAFLRQPNILEILRERQGQHTMSRQLREKINILRVEGVPALERLGHD 998
                                            .|.|:.::
Zfish   457 --------------------------------PRTGKSSL------------------------- 464

  Fly   999 LQLTILLSLFEQEIMGFVPIEERSPQGSPVLNSRMPQRAPPPFRRDFEAKL---RSFYRKLESKG 1060
                                 |..||.:.|              |||:||:   |.:.::|..
Zfish   465 ---------------------ENGPQITYV--------------RDFKAKVHYFRFWCQQLSM-- 492

  Fly  1061 YGQGPHKLKLHIRRSHLLEDAFRRIMSANKKDLQRGRLAVLWDTEEGLDYGGPSREFFFLLSREL 1125
                |..:|:|:.|..|.||:|::||..:.:||:| ||.:::..||||||||.:||:|||||.|:
Zfish   493 ----PQHIKIHVSRKTLFEDSFQQIMGCHPQDLRR-RLWIIFPGEEGLDYGGVAREWFFLLSHEV 552

  Fly  1126 FNPYYGLFEYSANDTYTVQVSPLSAFVDNCHDWFRFSGRVLGLALVHQYLLDAFFTRPFYKALLR 1190
            .||.|.||||:..|.|.:|::|.|:...:...:|:|.||.:.:||.|...:|..|:.||||.:|.
Zfish   553 LNPMYCLFEYAGKDNYCLQINPASSINPDHLKYFKFIGRFIAMALFHGKFIDTGFSLPFYKRILN 617

  Fly  1191 LPVALSDLESLDNEFHQSLQWIRDNDIGTGVDLGLTFCVTEELLGSVVDRELKPGGKNIIINEKN 1255
            .|:||.||||:|.||:.||.||:||:| ....|.:.|.|.:|:||.|...||||.|.||.:.|:|
Zfish   618 KPLALKDLESIDPEFYNSLIWIKDNNI-EECGLEMFFSVDKEILGEVTTHELKPDGGNIQVTEEN 681

  Fly  1256 KKEYLERMIKWRLERGVQEQTESLVRGFYEVIDSRLVSVFDARELELVIAGTAEIDTNDWRLNTE 1320
            |:||:..:.:|||.|||:|||::.:.||.:|:..:.:..|||:|||:::.|..|||..||:.|:.
Zfish   682 KEEYIRLVAEWRLSRGVEEQTQAFLEGFNDVLPQQYLQYFDAKELEVMLCGMQEIDLGDWQRNSI 746

  Fly  1321 YRSGYHDNHQVIVWFWQVIERFSNEQRLRLLQFVTGTSSIPYEGFSALRGSTGPRRFCIEKWGKP 1385
            ||. |..:.:.||||||.::...||:|:|||||||||..:|..||:.|.||.||::|||||.||.
Zfish   747 YRH-YARSSKQIVWFWQFVKEMDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKE 810

  Fly  1386 NALPRAHTCFNRLDLPPYPTPELLYEKLLLAVEETNTFGIE 1426
            |.|||:||||||||||||.:.|.|.||||.|:|||..||.|
Zfish   811 NWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 851

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hecw	NP_572574.2	PHA03247	<411..562	CDD:223021	35/159 (22%)
		WW	638..667	CDD:459800	17/28 (61%)
		HECW1_helix	738..794	CDD:465766	7/57 (12%)
		HECTc	1068..1424	CDD:238033	179/355 (50%)
itcha	NP_001274024.1	C2_E3_ubiquitin_ligase	2..125	CDD:175988	29/150 (19%)
		WW	276..305	CDD:459800	10/86 (12%)
		HUL4	<307..851	CDD:227354	251/800 (31%)