DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Fum4 and FH

DIOPT Version :9

Sequence 1:	NP_723527.1	Gene:	Fum4 / 318995	FlyBaseID:	FBgn0051874	Length:	484	Species:	Drosophila melanogaster
Sequence 2:	NP_000134.2	Gene:	FH / 2271	HGNCID:	3700	Length:	510	Species:	Homo sapiens

Alignment Length:	462	Identity:	275/462 - (59%)
Similarity:	349/462 - (75%)	Gaps:	3/462 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    24 RVEYDSMGAVHIPLDRMFGPQTMRSLMKFPIGGVEERMPRPLIKALGIVKKSAAETNKIHCLEEH 88
            |:|||:.|.:.:|.|:.:|.||:||.|.|.||||.||||.|:|||.||:|::|||.|:.:.|:..
Human    51 RIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPK 115

  Fly    89 LCDAISKACDDVISGKLYDEEHFPLVIWQDGSGEHTNMNVNEVICNRAIEILGGQMGSKEPVDPN 153
            :.:||.||.|:|..|||.|  |||||:||.|||..|||||||||.|||||:|||::|||.||.||
Human   116 IANAIMKAADEVAEGKLND--HFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPN 178

  Fly   154 EHVNMAQSSHDTFSTAVRIAVAMQLQETLYPSLRTFIDLLGKKSNDWMDLIKIGRTHLMDAVPLS 218
            :|||.:|||:|||.||:.||.|:::.|.|.|.|:...|.|..||.::..:|||||||..|||||:
Human   179 DHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLT 243

  Fly   219 LGQEFSGYQQQLVNGRTRLDCAMCRLYQLPMGGTSVGTKVDTKAEYSAQCIKRIAELTFLPFVES 283
            ||||||||.||:....||:..||.|:|:|..|||:|||.::|:..::.:...::|.||.||||.:
Human   244 LGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTA 308

  Fly   284 PNFFESISACDCLVELHGELNTIAASVMKIANDIRFLGSGPRCGFGELHLPENEPGSSIMPGKVN 348
            ||.||:::|.|.||||.|.:||.|.|:|||||||||||||||.|.|||.||||||||||||||||
Human   309 PNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVN 373

  Fly   349 PTQCEAMSMICAQVMGNHVAVSMGGSSGHFQLNTFMPMIASNVLRSITLLGDGMKSFCTNCLEGI 413
            |||||||:|:.||||||||||::|||:|||:||.|.||:..|||.|..||||...||..||:.||
Human   374 PTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGI 438

  Fly   414 EPNRSKIGSIVKESLMLVTALSPHIGYERSAAIAKAAHHNGTTLEQEAI-LDGIQREDFREWVQP 477
            :.|..:|..::.|||||||||:|||||:::|.|||.||.||:||::.|| |..:..|.|.|||:|
Human   439 QANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKP 503

  Fly   478 SKMLGPE 484
            ..||||:
Human   504 KDMLGPK 510

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Fum4	NP_723527.1	fumC	22..483	CDD:234779	273/459 (59%)
Fum4	NP_723527.1	Fumarase_classII	24..480	CDD:176465	271/456 (59%)
FH	NP_000134.2	fumC	49..510	CDD:234779	274/460 (60%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P05042	145..147		1/1 (100%)
		Substrate binding (B site). /evidence=ECO:0000250\|UniProtKB:P05042	176..179		1/2 (50%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P05042	186..188		1/1 (100%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P9WN93	371..373		1/1 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165159915
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S145
OMA	1	1.010	-	-		QHG61095
OrthoDB	1	1.010	-	-		D1022919at2759
OrthoFinder	1	1.000	-	-		FOG0002459
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101016
Panther	1	1.100	-	-	O	PTHR11444
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1641
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	11	10.670