DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Fum4 and Fh1

DIOPT Version :9

Sequence 1:	NP_723527.1	Gene:	Fum4 / 318995	FlyBaseID:	FBgn0051874	Length:	484	Species:	Drosophila melanogaster
Sequence 2:	NP_034339.2	Gene:	Fh1 / 14194	MGIID:	95530	Length:	507	Species:	Mus musculus

Alignment Length:	462	Identity:	271/462 - (58%)
Similarity:	347/462 - (75%)	Gaps:	3/462 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    24 RVEYDSMGAVHIPLDRMFGPQTMRSLMKFPIGGVEERMPRPLIKALGIVKKSAAETNKIHCLEEH 88
            |||:|:.|.:.:|.|:.:|.||:||.|.|.|||..||||.|:|:|.||:|::|||.|:.:.|:..
Mouse    48 RVEFDTFGELKVPTDKYYGAQTVRSTMNFKIGGATERMPIPVIQAFGILKRAAAEVNQEYGLDPK 112

  Fly    89 LCDAISKACDDVISGKLYDEEHFPLVIWQDGSGEHTNMNVNEVICNRAIEILGGQMGSKEPVDPN 153
            :..||.||.|:|..|||.|  |||||:||.|||..|||||||||.|||||:|||::|||:||.||
Mouse   113 IASAIMKAADEVAEGKLND--HFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKKPVHPN 175

  Fly   154 EHVNMAQSSHDTFSTAVRIAVAMQLQETLYPSLRTFIDLLGKKSNDWMDLIKIGRTHLMDAVPLS 218
            :|||.:|||:|||.||:.||.|:::.:.|.|.|:...|.|..||.::..:|||||||..|||||:
Mouse   176 DHVNKSQSSNDTFPTAMHIAAAVEVHKVLLPGLQKLHDALSAKSKEFAQVIKIGRTHTQDAVPLT 240

  Fly   219 LGQEFSGYQQQLVNGRTRLDCAMCRLYQLPMGGTSVGTKVDTKAEYSAQCIKRIAELTFLPFVES 283
            ||||||||.||:.....|:..||.|:|:|..|||:|||.::|:..::.:...::|.||.||||.:
Mouse   241 LGQEFSGYVQQVQYAMVRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTA 305

  Fly   284 PNFFESISACDCLVELHGELNTIAASVMKIANDIRFLGSGPRCGFGELHLPENEPGSSIMPGKVN 348
            ||.||:::|.|.||||.|.:||.|.|:|||||||||||||||.|.|||.||||||||||||||||
Mouse   306 PNKFEALAAHDALVELSGAMNTAACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVN 370

  Fly   349 PTQCEAMSMICAQVMGNHVAVSMGGSSGHFQLNTFMPMIASNVLRSITLLGDGMKSFCTNCLEGI 413
            |||||||:|:.||||||||||::|||:|||:||.|.||:..|||.|..||||...||..||:.||
Mouse   371 PTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTDNCVVGI 435

  Fly   414 EPNRSKIGSIVKESLMLVTALSPHIGYERSAAIAKAAHHNGTTLEQEAI-LDGIQREDFREWVQP 477
            :.|..:|..::.|||||||||:|||||:::|.|||.||.||:||::.|| |..:..|.|.|||:|
Mouse   436 QANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKP 500

  Fly   478 SKMLGPE 484
            ..||||:
Mouse   501 KDMLGPK 507

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Fum4	NP_723527.1	fumC	22..483	CDD:234779	269/459 (59%)
Fum4	NP_723527.1	Fumarase_classII	24..480	CDD:176465	267/456 (59%)
Fh1	NP_034339.2	fumC	46..507	CDD:234779	270/460 (59%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P05042	142..144		1/1 (100%)
		Substrate binding (B site). /evidence=ECO:0000250\|UniProtKB:P05042	173..176		1/2 (50%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P05042	183..185		1/1 (100%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P9WN93	368..370		1/1 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850285
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S145
OMA	1	1.010	-	-		QHG61095
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002459
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_101016
Panther	1	1.100	-	-	O	PTHR11444
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1641
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	11	10.660