Protein Alignment Rubicon and PLEKHM1

DIOPT Version :10

• Sequence 1: NP_572496.1
• Gene: Rubicon / 31803
• FlyBaseID: FBgn0030055
• Length: 827
• Species: Drosophila melanogaster
• Sequence 2: NP_055613.1
• Gene: PLEKHM1 / 9842
• HGNCID: 29017
• Length: 1056
• Species: Homo sapiens
• Alignment Length: 1094
• Identity: 221/1094 - (20%)
• Similarity: 360/1094 - (32%)
• Gaps: 378/1094 - (34%)

  Fly     9 TLASDAIEGAVYAAGEALHHQHDHPQ---QQQQQQQQQQL------------ARNSLLADLRRST 58
            |:.| |:| ||:..|  ||.:|...:   ::::...|:.|            ....::::|...|
Human    49 TMCS-ALE-AVFIHG--LHAKHIRAEAGGKRKKSAHQKPLPQPVFWPLLKAVTHKHIISELEHLT 109

  Fly    59 ---------NFWFRAAKSTDG-----LQLLAQSCTRILQHGLLQPVELRRLADDFEFLLASAGKQ 109
                     ..|.|.|.: ||     |:||.|...|:  |...||..|.|.|::.||||
Human   110 FVNTDVGRCRAWLRLALN-DGLMECYLKLLLQEQARL--HEYYQPTALLRDAEEGEFLL------ 165

  Fly   110 QQQTKPQPYRPDYAARDSASMVNGV----------EEFLGEWTTR--CLRARC-LSQCLQTLVSD 161
                               |.:.|:          ...|.|||..  .|...| ||:......|.
Human   166 -------------------SFLQGLTSLSFELSYKSAILNEWTLTPLALSGLCPLSELDPLSTSG 211

  Fly   162 PEL-----------------MELYYPSEEAFLRRSGDATALFVCL-------TAVQLNQSGLLSQ 202
            .||                 :|:::...:  :||:...||..:.|       .:..||....|.|
Human   212 AELQRKESLDSISHSSGSEDIEVHHSGHK--IRRNQKLTASSLSLDTASSSQLSCSLNSDSCLLQ 274

  Fly   203 LEVRHQVRHRRTCSQPNFSISPKL---QAVPEERLQKRIFLR----RLKSLP--NLSQEDEELDD 258
            ........|   |.:| .|....|   .|...:|..:.:.|.    ::.|:|  .||||.|....
Human   275 ENGSKSPDH---CEEP-MSCDSDLGTANAEDSDRSLQEVLLEFSKAQVNSVPTNGLSQETEIPTP 335

  Fly   259 RAAFRRRCQTFSSSRSHKLRED---HLDA-----PSTSSCSSHD------------SPRRIQLIN 303
            :|          |...|.|...   |.:|     |:.::..:.|            ||..:|
Human   336 QA----------SLSLHGLNTSTYLHCEAPAEPLPAQAASGTQDGVHVQEPRPQAPSPLDLQ--- 387

  Fly   304 CDDIKIWTDQTPQEVPEEVHEKLQQAS---KASTSVGGYLGSFFGSP--PLYTSW---------- 353
             ..::..:.|.|.....|...::.|.:   ||....|..|.....||  |...||
Human   388 -QPVESTSGQQPSSTVSETAREVGQGNGLQKAQAHDGAGLKLVVSSPTSPKNKSWISEDDFYRPS 451

  Fly   354 -------------------------FHRGVGDDLNASGDGGMLD--------------------- 372
                                     .||....:...:...|.||
Human   452 REQPLESASDHPIASYRGTPGSRPGLHRHFSQEPRKNCSLGALDQACVPSPGRRQAQAAPSQGHK 516

  Fly   373 TFLPVNGRKL-----------------------------------------KKQHTLFEGVSMLD 396
            :|..|:.|::                                         .::||..|..|:|
Human   517 SFRVVHRRQMGLSNPFRGLMKLGTVERRGAMGIWKELFCELSPLEFRLYLSNEEHTCVENCSLL- 580

  Fly   397 EAASSTACISSAPWDIQGS----AGDSTTSTTASSSVSITPGSDKMDQQSLASFLQMSRQTHNNT 457
                  .|.|..|....|.    ......:..|||            |.....:|...|:.....
Human   581 ------RCESVGPAHSDGRFELVFSGKKLALRASS------------QDEAEDWLDRVREALQKV 627

  Fly   458 QLEKENAHFRIS------------EACITAIEHVKWSRRLATPAGP-------TSHNLSEPNL-- 501
            :.::|:....:.            :.|::.      |..|:.||..       :|..:.||:.
Human   628 RPQQEDEWVNVQYPDQPEEPPEAPQGCLSP------SDLLSEPAALQGTQFDWSSAQVPEPDAIK 686

  Fly   502 ------------MPYAEQIPGGGMQ-------------NHSAEAVG------------------- 522
                        |||...:....::             :|..|.:.
Human   687 ESLLYLYMDRTWMPYIFSLSLEALKCFRIRNNEKMLSDSHGVETIRDILPDTSLGGPSFFKIITA 751

  Fly   523 ---LQLISRFSEQ-----QLPRLGHLKWLVSEQEAPQQLLPLPTPKQDHEQASLTRGTSSWAPPR 579
               |:|.:..:|:     .|.|    |.|.|..|..::.:.|.....::.|..|...|      |
Human   752 KAVLKLQAGNAEEAALWRDLVR----KVLASYLETAEEAVTLGGSLDENCQEVLKFAT------R 806

  Fly   580 QQIIFTEH----PSESRSKVLQKQNHRCAGCGMRVAKHLQQHFRYCTYLGKYLCTGCHRNQISAI 640
            :.....::    |.|   |.|..|...||||..::.....:. :.|.:.|.|.|..||::..|.|
Human   807 ENGFLLQYLVAIPME---KGLDSQGCFCAGCSRQIGFSFVRP-KLCAFSGLYYCDICHQDDASVI 867

  Fly   641 PARILRSWDFRCYPVCSFAYRLIEQMYAFPLFHVPDLNSQLYKHKE-LAKARKRRLQLQAVKGFI 704
            ||||:.:||....|:|..|.:.:.|:.|.||.::..:|:.||:|.| :....:||.||:.:..::
Human   868 PARIIHNWDLTKRPICRQALKFLTQIRAQPLINLQMVNASLYEHVERMHLIGRRREQLKLLGDYL 932

  Fly   705 ANCRF-ATREQSFFNAIPAHITQDPDMWSMCDFVDVQNTSMNRSIKEVIALSEQHVHNCVLCTGR 768
            ..||. |.:|.|.......::.:.|..:|:.|...:.:......:|.:|..:.|||::|.|||.|
Human   933 GLCRSGALKELSKRLNHRNYLLESPHRFSVADLQQIADGVYEGFLKALIEFASQHVYHCDLCTQR 997

  Fly   769 AFVCEHCKGGELIYPWQ-RKVQRCDRCGACFHNGCWKVRSRCRNQNDPCPRCSR 821
            .|:|:.|:..::|:|:: ....||..|...||..|..|..:      .||||:|
Human   998 GFICQICQHHDIIFPFEFDTTVRCAECKTVFHQSCQAVVKK------GCPRCAR 1045

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rubicon	NP_572496.1	RUN_RUBCN	50..207	CDD:439048	45/207 (22%)
		zf-RING_9	619..824	CDD:464030	65/206 (32%)
PLEKHM1	NP_055613.1	RUN_PLEKHM1	18..188	CDD:439041	37/170 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	215..245		3/31 (10%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	277..303		6/29 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	360..422		11/65 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	451..483		2/31 (6%)
		PH_PLEKHM1	499..629	CDD:241475	18/148 (12%)
		LIR. /evidence=ECO:0000305	632..638		1/5 (20%)
		Interaction with RAB7A. /evidence=ECO:0000269|PubMed:25500191	654..1056		101/418 (24%)
		PH	685..777	CDD:214574	11/95 (12%)
		zf-RING_9	846..1048	CDD:464030	65/206 (32%)