Protein Alignment Rubicon and Rufy2

DIOPT Version :10

• Sequence 1: NP_572496.1
• Gene: Rubicon / 31803
• FlyBaseID: FBgn0030055
• Length: 827
• Species: Drosophila melanogaster
• Sequence 2: XP_038955024.1
• Gene: Rufy2 / 690777
• RGDID: 1592188
• Length: 737
• Species: Rattus norvegicus
• Alignment Length: 688
• Identity: 110/688 - (15%)
• Similarity: 217/688 - (31%)
• Gaps: 245/688 - (35%)

  Fly    81 ILQHGLLQPVELRRLADDFEFLLASAGKQQQQTKPQPYRPDYAARDSASMVNGVEEFLGE---WT 142
            :::|.|...:::|:....:...:....:..::..|:......:.||    :.|::..||.   |.
  Rat   180 VMEHCLKHGLKVRKSFLSYNKTIWGPLELVEKLYPEAEEIGASVRD----LPGLKTPLGRARAWL 240

  Fly   143 TRCLRARCLSQCLQTLVSDPELMELYYPSEEAFLRRSGDA-TALFVCLTAVQLN----------Q 196
            ...|..:.::..|:.|:...||:..:|......:...|.. ..|.|.|..:..|          |
  Rat   241 RLALMQKKMADYLRCLIIQRELLSEFYEYHALMMEEEGAVIVGLLVGLNVIDANLCVKGEDLDSQ 305

  Fly   197 SGLL-------SQLEVRHQVRHRRTCS-------------QPNFSISP----------------- 224
            .|::       ::.|:.::.|:.:..:             |.|.::|.
  Rat   306 VGVIDFSMYLKNEEEIGNKERNVQIAAILDQKNYVEELNRQLNSTVSSLHSRVDSLEKSNTKLIE 370

  Fly   225 ----------KLQAVPEE----RLQKRIFLRRLKSLPNLSQEDEELDDRAAFRRRCQTFSSSR-- 273
                      |||   ||    |.:..:.|.|.:....:::.|.|.:        .||:..||
  Rat   371 ELAIAKNNIIKLQ---EENHQLRSENELILMRTRQHLEVTKVDVETE--------LQTYKHSRQG 424

  Fly   274 --------------SHKLREDHLDAPSTSSCSSHDSPRRIQLINCD-----DIKIWTDQTPQEVP 319
                          ..:||:|..:..|......|:....::|:..|     |..|...|..:||.
  Rat   425 LDEMYDDARRQLRDESQLRQDVENELSVQVGMKHEIELAMKLLEKDIHEKQDTLIGLRQQLEEVK 489

  Fly   320 E---EVHEKLQQASKASTSVGGYLGSFFGSPPLYTSWFHRGVGDDLNASGDGGMLDTFLPVNGRK 381
            .   |:::|||                                    .|.||
  Rat   490 AINIEMYQKLQ------------------------------------GSEDG------------- 505

  Fly   382 LKKQHTLFEGVSMLDEAASSTACISSAPWDIQGSAGDSTTSTTASSSVSITPGSDKMDQQSLASF 446
            ||:::   |.::.|:|   .|..|::|...:     :........:.:......:|..|:.|:..
  Rat   506 LKEKN---EIIARLEE---KTNKITTAMRQL-----EQRLQQAEKAQMEAEDEDEKYAQECLSKS 559

  Fly   447 LQMSRQ-THNNTQLEKENAHFRISEACITAIEHVKWSRRLATPAGPTSHNLSEPNLMPYAEQIPG 510
            ..:.|| :....||.:.....:|.:         :|.:.|..........||.
  Rat   560 DSLQRQISQKEQQLVQLETDLKIEK---------EWRQTLQEDLQKEKDVLSH------------ 603

  Fly   511 GGMQNHSAEAVGLQ---LISRFSEQQLPRLGHLKWLVSEQEAPQQL--------LPLPTPKQDHE 564
              :::.:.:.:.|:   |..:...|||.|:.|     .:::|.|:|        |.:...|:.::
  Rat   604 --LRHETQKIISLKKEFLNLQDENQQLKRIYH-----EQEQALQELGSKLCESKLKIDDIKEANK 661

  Fly   565 QASLTRGTSSWAPPRQQIIFTEHPSESRSKV------LQKQNHRCAGCGMRVAKHLQQHFRYCTY 623
            ..             |.:::.:....:..|:      |.|:.|.|..|
  Rat   662 AL-------------QGLVWLKDKDATHCKLCEKEFSLSKRKHHCRNC----------------- 696

  Fly   624 LGKYLCTGCHRNQI----SAIPARILRSWDFRCYPVCS 657
             |:..|..|..|::    |..|.|:..|........||
  Rat   697 -GEIFCNACSDNELPLPSSPKPVRVCDSCHAMLIQRCS 733

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rubicon	NP_572496.1	RUN_RUBCN	50..207	CDD:439048	24/146 (16%)
		zf-RING_9	619..824	CDD:464030	10/43 (23%)
Rufy2	XP_038955024.1	RUN_RUFY2	141..296	CDD:439057	21/119 (18%)
		Smc	<381..>664	CDD:440809	65/394 (16%)
		FYVE_like_SF	665..734	CDD:333710	16/87 (18%)