Protein Alignment Rubicon and Plekhm1

DIOPT Version :10

• Sequence 1: NP_572496.1
• Gene: Rubicon / 31803
• FlyBaseID: FBgn0030055
• Length: 827
• Species: Drosophila melanogaster
• Sequence 2: NP_001009677.1
• Gene: Plekhm1 / 303584
• RGDID: 1308010
• Length: 1059
• Species: Rattus norvegicus
• Alignment Length: 1126
• Identity: 221/1126 - (19%)
• Similarity: 347/1126 - (30%)
• Gaps: 472/1126 - (41%)

  Fly    21 AAGEALHHQHDHPQQQQQ-QQQQQQLARNSLLADLRR----STNF-----WFRAAKSTDG----- 70
            |.|:...|.|..|..|.. ....:.:....:::||..    :|:.     |.|.|.: ||
  Rat    70 AGGKRKKHTHQKPLPQPVFWPLLKAVTHKHIISDLEHLVFINTDVGRCRAWLRLALN-DGLMECY 133

  Fly    71 LQLLAQSCTRILQHGLLQPVELRRLADDFEFLLASAGKQQQQTKPQPYRPDYAARDSASMVNGV- 134
            |:||.|...|:.::  .||..|.|.|::.||||                         |.:.|:
  Rat   134 LKLLLQEPARLCEY--YQPTALLRDAEEAEFLL-------------------------SFLQGLT 171

  Fly   135 ---------EEFLGEWTTR--CLRARC-LSQCLQTLVSDPELMELYYPSEEAFLRRSGDATALFV 187
                     ...|.|||..  .|...| ||:.....||..||..  ..|.::....||...
  Rat   172 SLSFELSYKSAILNEWTLTPLSLSGLCPLSELDPLTVSGAELQR--KESLDSISHSSGSED---- 230

  Fly   188 CLTAVQLNQSGLLSQLEVRHQVRHRRTCSQPNFSISPKLQAVPEERLQKRIFLRRLKSLPNLSQE 252
                :::..||        |::|..|..:..:.|
  Rat   231 ----IEVQHSG--------HKIRRDRKLTASSLS------------------------------- 252

  Fly   253 DEELDDRAAFRRRCQTFSSSRSHKLRE------DHLDAPSTSSCSSHDSPRRIQLINCDDIKIWT 311
               ||..::.:..|..  :|.|..|:|      ||.:.|     .|:||.  :...|.||    :
  Rat   253 ---LDTASSSQLSCSL--NSDSCLLQENGPKSPDHSEEP-----MSYDSD--LGTANADD----S 301

  Fly   312 DQTPQEVPEEVHEKLQQASKASTSVGGYLGSFFGSPPLYTSWFHRGVGDDLNASGDGGMLDTFL- 375
            |::.|||       |.:.|||..:          |.|               :||.....||.:
  Rat   302 DRSLQEV-------LSEFSKAQVN----------SAP---------------SSGPSQESDTPMF 334

  Fly   376 --PVNGRKL-KKQHTLFEGV--------------------------------------SMLDEAA 399
              |::...| ...|.||||.                                      |..|:..
  Rat   335 QTPLSLHSLANSTHLLFEGSEEPFPAHTSSGTSSGHKHQPQESPDMQPLGTAQAGPAGSTSDQQP 399

  Fly   400 SS----TACISSAPWDI--QGSAG-----DSTTSTTASSSVS--------------------ITP 433
            ||    .|...|.||..  .|..|     .|.||....|.:|                    |:|
  Rat   400 SSPVAGAADQGSEPWKALEYGRVGPKLVVSSPTSPKGKSWISEDDFCRPPKEHALKNTSDLCISP 464

  Fly   434 --GSDKMDQQSLASFLQMSRQTHNNTQLEKENAHFRISEACITAIEHVKWSRRLATPAGPTSH-- 494
              |:.::.......|.|..|::.:...|:|         ||:::::: :.::...:||....|
  Rat   465 LQGTPELRTALHGPFSQGPRKSCSLGALDK---------ACVSSLDY-RNAQTAPSPAVTQDHKN 519

  Fly   495 ---------NLSEP--NLMPYAEQIPGGGM---------------------------QNHS---A 518
                     .||.|  .||........|.|                           ::.|   .
  Rat   520 FCVVHRRQMGLSNPFRGLMKLGTVARRGAMGIWKEFFCELSPLELRLYLSDEERTCVESCSLLRC 584

  Fly   519 EAVG-------LQLISRFSEQQLPRLGHLK-----WLVSEQEA-----PQQ-------------- 552
            ||||       .:|:  ||.::|......:     ||...:||     |||
  Rat   585 EAVGPAHSDGRFELV--FSGKKLALRASSQDEAEDWLDRVREALQKVRPQQEEEWVNIQYPDQAE 647

  Fly   553 ---------------LLP----------------LPTPKQDHEQASLTRGTSSWAP--------- 577
                           |||                :|.|....|.........:|.|
  Rat   648 DSPEAPPDNLPPYSALLPEHAGAQGIQPNWTSAQVPEPDAIKESLLYLYADRTWIPYIFSLSLES 712

  Fly   578 -------PRQQIIFTEHPSES-------------------------------------------- 591
                   ..::::...|..|:
  Rat   713 LKCFRVRNNEKMLSDSHGVETIRDILPDTSLGGPAFFKIITAKAVLKLQAKNTEEAAHWRDLVRK 777

  Fly   592 ---------------------------------------------RSKVLQKQNHRCAGCGMRVA 611
                                                         ..|.|..|...||||..::.
  Rat   778 VLASYLESVQEAVTLAGSLDENCQEVLKFATRENGFLLQYLVAIPTEKGLDSQGCFCAGCSRQIG 842

  Fly   612 KHLQQHFRYCTYLGKYLCTGCHRNQISAIPARILRSWDFRCYPVCSFAYRLIEQMYAFPLFHVPD 676
            ....:. :.|.:.|.|.|..||::..|.|||||:.:||....|||..|.:.:.|:.|.||.::..
  Rat   843 FSFVRP-KLCAFSGLYYCDFCHQDDASVIPARIIHNWDLTKRPVCRQALKFLAQIRAQPLINLQL 906

  Fly   677 LNSQLYKHKE----LAKARKRRLQLQAVKGFIANCRF-ATREQSFFNAIPAHITQDPDMWSMCDF 736
            :|:.||:|.|    :.::|:   ||:.:..::..||. |.:|.|...:...::.:.|..:|:.|.
  Rat   907 VNASLYEHVERMHLIGRSRE---QLKLLGDYLGLCRSGALKELSKRLSHRNYLLESPHKFSVADL 968

  Fly   737 VDVQNTSMNRSIKEVIALSEQHVHNCVLCTGRAFVCEHCKGGELIYPWQ-RKVQRCDRCGACFHN 800
            ..:........:|.:|..:.|||::|.|||.|.|:|:.|...::|:|:: ....||..|...||.
  Rat   969 QQIAEGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICHHQDIIFPFEFDTTVRCAECRTVFHQ 1033

  Fly   801 GCWKVRSRCRNQNDPCPRCSR 821
            .|..|..:      .||||:|
  Rat  1034 SCQAVVRK------GCPRCAR 1048

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rubicon	NP_572496.1	RUN_RUBCN	50..207	CDD:439048	41/183 (22%)
		zf-RING_9	619..824	CDD:464030	65/209 (31%)
Plekhm1	NP_001009677.1	RUN_PLEKHM1	17..187	CDD:439041	31/144 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	218..244		7/41 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	272..334		25/104 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	354..411		5/56 (9%)
		PH_PLEKHM1	508..631	CDD:241475	23/124 (19%)
		LIR. /evidence=ECO:0000250|UniProtKB:Q9Y4G2	634..640		0/5 (0%)
		Interaction with RAB7A. /evidence=ECO:0000250|UniProtKB:Q9Y4G2	657..1059		82/402 (20%)
		PH	688..780	CDD:214574	5/91 (5%)
		zf-RING_9	849..1051	CDD:464030	65/209 (31%)