Protein Alignment Crag and dennd4a

DIOPT Version :10

• Sequence 1: NP_525080.3
• Gene: Crag / 31800
• FlyBaseID: FBgn0025864
• Length: 1671
• Species: Drosophila melanogaster
• Sequence 2: XP_005159157.1
• Gene: dennd4a / 558211
• ZFINID: ZDB-GENE-060503-285
• Length: 1724
• Species: Danio rerio
• Alignment Length: 1822
• Identity: 647/1822 - (35%)
• Similarity: 941/1822 - (51%)
• Gaps: 260/1822 - (14%)

  Fly     1 MEEK--RIADYFVVAGMPENPQLLQENSFNDSGRLRAATTIE-PITDIGVYFPLLGEEVPEGYEI 62
            ||||  |:||||||||:..:.:.|:|:...:.|..|::..:: ||||:.|....||||||.||..
Zfish     1 MEEKSLRVADYFVVAGLTVSSKPLEEDVHPEDGAHRSSAQLKAPITDVAVVIRSLGEEVPRGYTC 65

  Fly    63 LSHTPTGLQANLNHGSVRTTDCYIYFRRGKDRPPLVDIGVLYDGHERIMSDAEIVAETPGGRVAN 127
            :..||:||.|.||.||:.....::.:|||:|:|||.|:|:||:..|::..|.:|:..||.||.||
Zfish    66 VECTPSGLPAELNGGSLMGPQIFLCYRRGRDKPPLTDLGILYEWKEKLKPDCQIIQTTPSGRPAN 130

  Fly   128 VNNSSA-KTFLTYRRARADMPCNELVVTELCVIVQSKGERAPHAFCLIYKTLNKGYVGSDVYLCY 191
            ::.||: :.::|||||..:.....|.||::|||:..|||..|||||.:.|.||....||.|||||
Zfish   131 ISGSSSQRIYITYRRAAENHSHATLAVTDICVIIPGKGETPPHAFCKVDKNLNSSMWGSSVYLCY 195

  Fly   192 KKSMYRPKHISYKPEILLRYPTFDHTDFPLNLCPSVPLFCLPMGASLEAWPHVNGTEKRKPISPV 256
            |||:.:...:::|..:|.|||..|:..|||.  .||||||||||||:|.|     :...|...||
Zfish   196 KKSLAKTNTLAFKAGLLSRYPEEDYDSFPLP--ESVPLFCLPMGASIECW-----SSHTKYSLPV 253

  Fly   257 FSTFVLTVSDGTYKVYGSALTFYEDYDESKLSAEQKELLGWDEEF---GAQHSLHMIKAICLLSH 318
            |||||||.:.|. ||||:|:.|||.|.|..|:.:|:..||.....   ....::|..|:||||||
Zfish   254 FSTFVLTGASGE-KVYGAAIQFYEPYPEEHLTEKQRSQLGLQANGIRPDGSMTVHTNKSICLLSH 317

  Fly   319 HPFGDTFDKWLKYLHRMVLYDVNIP--IPVERYITQLLDEVPFPA---PSIHLQLSSESNDRILL 378
            .||.|.|..:|.:|:|   |.::.|  :|:|::|:..:.:||||:   |.|.:|||  .:|.::|
Zfish   318 WPFFDAFRNFLTFLYR---YSISGPHTLPIEKHISHFMHKVPFPSSQRPRILVQLS--PHDSLML 377

  Fly   379 TQPEDSPLPRSGAGFHILLQNLGTDNCLHVLLLALTEQKILIHSLRPATLTAVAEAIVSLLFPFK 443
            :||..||||.||.....||.|||..|...:|:||:||.|||:||||||.||:|.||:||::|||.
Zfish   378 SQPVSSPLPLSGGRLSTLLLNLGPKNAATLLVLAVTEHKILVHSLRPAVLTSVTEALVSMIFPFH 442

  Fly   444 WQCPYIPLCPLGLAEVLHAPLPYLIGVDSRFFDLYEPPTDVTCIDLDTNNISLCESQRHLSPKLL 508
            |.|||||||||.||:||.||.|:::|||||:|||||||||::|:|||||.|:..|.:|.|:.|:|
Zfish   443 WPCPYIPLCPLALADVLSAPCPFIVGVDSRYFDLYEPPTDISCVDLDTNTITHKEERRSLTWKIL 507

  Fly   509 PKRAARLLRQTLTELENAKPISYDST---NSLDRDIRKR------KRELVLEQRIQEAFLLFMAS 564
            |::|.:.|..||:.|.......|..:   ..::..:.:|      |....||..||||||.|||:
Zfish   508 PRKACKHLINTLSNLHQQLDEGYQMSREEGQMEAFMSEREPGSGGKSLETLELEIQEAFLRFMAA 572

  Fly   565 ILRGYRDFLVPISKAPSVGATDPSALFQLKAFLRSRDKSHQKFFELMMKTQMFIRFIEERSFVSD 629
            ||:|||.:|:||::|||..|||.|:||.|:.||:|||:|||||:.||.|||||||||||.|||||
Zfish   573 ILKGYRSYLLPITQAPSEKATDASSLFDLQGFLKSRDRSHQKFYSLMTKTQMFIRFIEECSFVSD 637

  Fly   630 GDHGLSFFDECAEKV------GNYD-ETPAQLHLVDWDTGQNSERTKYIFPPD-SVTPAGSQPGG 686
            .|..|:|||:|.:|:      |..| :.|....|::.|....||.|.:|.||: ...|.|.:|
Zfish   638 KDASLAFFDDCVDKLFGTEKGGKVDSDKPEDTRLIELDKSHRSEHTVFISPPELPALPEGEEP-- 700

  Fly   687 GGGGLAYNYENF-TLQPEL------LQSTKKTALSKFLQLQLNASLSPGSPIA--RRTKHEIKLS 742
               .:.|:|..| .|..:|      |:..|.|:..:        .:||.||.|  ||::.|||.:
Zfish   701 ---PMVYSYSGFPALNAQLCEHQDGLRVPKATSALR--------HISPISPAAIFRRSQQEIKSA 754

  Fly   743 QKMASRCQQHPEAWSKYLLATCYSLYFLILPSMVLDPRHAGKEPEILRAAYDVLVRASRLKI-TC 806
            |:||......|:.|||.||..|:.|:|:.||:.| ...|:  :...|..|||||......|: ..
Zfish   755 QRMAKTYSSIPQMWSKCLLRHCHGLWFICLPAYV-SACHS--KVRALHTAYDVLRNMQDKKLQPP 816

  Fly   807 DEFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWN 871
            ||.|||::|||||.:..||||||:.:.||::|||.||:|||:|||.||||.|| .||......|.
Zfish   817 DEVCYRVLMQLCGQYGQPVLAVRVLFEMKKAGVQPNAITYGYYNRAVLESTWP-SSTRGGYFLWG 880

  Fly   872 RIKNVVLGAAQFRKAGKQRAASKVNKSLSASQDQNLSTLETVDGQSRTSLASSSGDGGGHGLLDF 936
            :::|||.|...|::..:::.        :..:|.:||....:|..|..||.||:...      :.
Zfish   881 KLRNVVRGVVLFKQVWRRQT--------THPKDTHLSDASDLDSVSHGSLDSSNDSA------ER 931

  Fly   937 AAFDRLRNKLGSIVRQTVTGGNNETMGDAVNNTGLLIPGELSAPNTPTYGGDTEILAK------- 994
            .:.|....|:.|...::.|||.::...|:::...:...|.....::|      :|..|
Zfish   932 VSIDTDFTKMDSSDDRSSTGGQSDQGYDSLSKEEVRFGGGEEQNSSP------DIKEKKDRDSCP 990

  Fly   995 ALQQQQPRKQIMSIGGGDDDDE--DEDEDEYTAGSPSTPQKQLEAGADDLEYAGGGDYEADEED- 1056
            :.|.:..:|:..|:..|....|  .|....::...|.|.:      .:.|:..|......:..|
Zfish   991 SSQSELSKKEDSSLAAGIHPPEINKESSQAFSNTIPKTKR------PNSLDIFGEKPVRPNTLDL 1049

  Fly  1057 --EDEVDEHVAAQ---RARQRVQSPTKIS------------------------------------ 1080
              :..||....||   .|.:.|.||.|:|
Zfish  1050 TPDKPVDSVPVAQTQTEALEGVVSPPKVSVERKASCTVTMEKTVAMESGHTGVERSMSCNSALSR 1114

  Fly  1081 --PRTPVTQN-DPLGAL-------NEEESAASATPTQETQQEQQHSQSQIDSSIYSDKPILFRGQ 1135
              .||.:... |||..:       .:|::|.:.:..::..:|..|..:.:.|.:         .|
Zfish  1115 VVKRTGIEAGFDPLSLMAAETHGHEDEDNAGTPSARRDLAEEIHHYMNNLSSPL---------SQ 1170

  Fly  1136 RSATFDESTQIGKSMHRSETM-------PVASSGVTNSLANIGSSLKFTFGRYSPARLSLKKDLK 1193
            ||.:.|.......|.|.|..|       |.||.........:.||.....||....|......|.
Zfish  1171 RSLSVDLKGHQTPSPHSSPCMSKIALGSPSASQLQPQPRTRLFSSPSLPQGRVRRVREQRPTSLA 1235

  Fly  1194 LPANIIENISSISPSLTGKKSNELIQGSL----SSIKSAANSLTKKFDEIKGVISANSTPTKTNN 1254
            .|       ||.:||.:....:.|:..:|    ||..||...:.:|...:...:|:.::..:..|
Zfish  1236 SP-------SSPTPSSSSFSMDSLLTPTLDVFKSSFLSAGKGVAEKASRLYSRLSSQTSIAQDLN 1293

  Fly  1255 GHHPHGLHHGHHHPHHHHHHHSQHGNAEQE-----EHDAAVHEEGKLRRVSSDLDPWGRLSESRK 1314
            ....               ..|..|:.:.:     |.|..|..||   ..|...|....:...||
Zfish  1294 SDRV---------------SVSSLGSGDPDCSSLIESDLCVDPEG---LSSPQRDAAQPVIRPRK 1340

  Fly  1315 SSYNNLVPLGENSSTGALHMHAFPAVP---------------DNLYSLTSENAADRDCDVLIQLT 1364
            |.:.:...|...|:...|...|....|               |:.:|..:::.|     |.::::
Zfish  1341 SPHRSNNCLESPSAAPRLFRQASLTGPSLTVMKALQTPDTCSDSSFSQFTQSYA-----VEVRMS 1400

  Fly  1365 TCSQCHNCSVLVYDEEIMSGWTAEDSNLNTTCHACNKLTVPFLSVQI------ER---------Q 1414
            :||:|:.|..||||||||:||||:|||||:||..|....:|.|:|.|      ||         |
Zfish  1401 SCSRCNTCESLVYDEEIMAGWTADDSNLNSTCPFCGSPFLPLLNVDITNMGEQERSPCQDTSSGQ 1465

  Fly  1415 VEESEQSDPLQDGKEQIAN-GNSHKTSLTVPYLNPLVLRKELENILTQEGDIALIKPEFVDEHPI 1478
            .|..::...:.|....::| ......::|||||:||||.||||::|..|||.|:.....||.|||
Zfish  1466 TEREDEPPKVYDASSTLSNKDKKDPETVTVPYLSPLVLWKELESLLENEGDQAISSAAIVDHHPI 1530

  Fly  1479 IYWNLLWLMERIESKTHLPEL-------CLPVPSDKEHIDPLSKVKTVHIQCLWDNLSLHTEASG 1536
            ::|||:|...|::..:.||.|       |...|..::.. .....|.|.:|.||||..||.:..
Zfish  1531 VFWNLVWFFRRLDLPSSLPGLYLTSKNYCRDTPQSQQSC-ASEDSKNVLVQILWDNCRLHQDHI- 1593

  Fly  1537 PPMYLLYRETQPTSPLIKALLTDQAQLNKNVIQQIISAIRCNDFATPLK---RLANERHKLKSNG 1598
            .|.|||::....::.:..:|..|:..::..::|.::..|:.||...|:.   :|...|     .|
Zfish  1594 QPCYLLWKSYCLSTAVSSSLNEDEQAVSLELLQTLVKNIQKNDVYQPMSLILQLLGSR-----LG 1653

  Fly  1599 VERSHSFYRDILFLALTAIGRSNVDLATFHREYAAVFDKLTERECNMYYRNQDLPPSASTIFCRA 1663
            ..|..|.|||||||:|.|:|:|::::..|.|||...:|:||..:..|.: |.|.||||..:.||.
Zfish  1654 FSRQRSLYRDILFLSLVALGKSSINIDAFDREYKTAYDRLTPSQVKMTH-NCDRPPSAGVMECRR 1717

  Fly  1664 YF 1665
            .|
Zfish  1718 TF 1719

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Crag	NP_525080.3	uDENN	189..279	CDD:214824	45/89 (51%)
		DENN	308..492	CDD:460461	104/188 (55%)
		dDENN	551..624	CDD:129037	50/72 (69%)
		PPR repeat	808..837	CDD:276811	17/28 (61%)
dennd4a	XP_005159157.1	uDENN	168..275	CDD:214824	59/114 (52%)
		DENN	307..491	CDD:460461	104/188 (55%)
		dDENN	559..632	CDD:129037	50/72 (69%)
		PPR_2	816..856	CDD:463778	23/39 (59%)
		PPR repeat	818..847	CDD:276811	17/28 (61%)