Protein Alignment Crag and Dennd4b

DIOPT Version :10

• Sequence 1: NP_525080.3
• Gene: Crag / 31800
• FlyBaseID: FBgn0025864
• Length: 1671
• Species: Drosophila melanogaster
• Sequence 2: XP_008759470.1
• Gene: Dennd4b / 361987
• RGDID: 1560320
• Length: 1515
• Species: Rattus norvegicus
• Alignment Length: 1790
• Identity: 585/1790 - (32%)
• Similarity: 832/1790 - (46%)
• Gaps: 420/1790 - (23%)

  Fly     2 EEK--RIADYFVVAGMPENPQLLQENSF--NDSGRLRAATTIEPITDIGVYFPLLGEEVPEGYEI 62
            ||:  |:.|||||||:..|...:.|.::  ..:|.||.....|||||:.|....||||||:||..
  Rat    14 EERPPRLVDYFVVAGLAGNGAPIPEETWVPEPTGPLRPPRPAEPITDVAVIARALGEEVPQGYTC 78

  Fly    63 LSHTPTGLQANLNHGSVRTTDCYIYFRRGKDRPPLVDIGVLYDGHERIMSDAEIVAETPGGRVAN 127
            :..:..|....|:.|.:..|...|.:|||:|:||||::||||||.||.....:::..||....||
  Rat    79 IQTSAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYDGKERPKPGFQVLDTTPYSHSAN 143

  Fly   128 V---NNSSAKTFLTYRRARADMPCNELVVTELCVIVQSKGERAPHAFCLIYKTLNKGYVGSDVYL 189
            :   .....:|:|.||||......:.|.:|:||:::.||||..||.:|.:.:.||.|..|..|:|
  Rat   144 LAPPGPGHPRTYLMYRRAAEGAGLHALGITDLCLVLPSKGESTPHTYCRLPRNLNPGMWGPAVFL 208

  Fly   190 CYKKSMYRPKHISYKPEILLRYPTFDHTDFPLNLCPSVPLFCLPMGASLEAWPHVNGTEKRKPIS 254
            |||..:.:...:.|:.|:|.|||..|:..|||.  .|||:|||||||::|.||    .:.:.|:
  Rat   209 CYKVGLAKANTLVYEAELLGRYPEEDNEAFPLP--ESVPVFCLPMGATIECWP----AQTKYPV- 266

  Fly   255 PVFSTFVLTVSDGTYKVYGSALTFYEDYDESKLSAEQKELLGW------DEEFGAQHSLHMIKAI 313
            |||||||||.:.|. ||||:||.|||.:..::||..|...||.      ....|.: ::...:||
  Rat   267 PVFSTFVLTGAAGD-KVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGR-AVRSRRAI 329

  Fly   314 CLLSHHPFGDTFDKWLKYLHRMVLYDVNIP--IPVERYITQLLDEVPFPAPS---IHLQLSSESN 373
            .:||..|....|..:|.:|:|   |.|:.|  :|:|.:|:..:..||||:|.   |.:|:|  ..
  Rat   330 AVLSRWPAFPAFRAFLTFLYR---YSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMS--PY 389

  Fly   374 DRILLTQPEDSPLPRSGAGFHILLQNLGTDNCLHVLLLALTEQKILIHSLRPATLTAVAEAIVSL 438
            |.:||.||..||||.|||.|..||||||.:..:.:||..|||.|:|:|||||..||:|.||:||:
  Rat   390 DNLLLCQPVSSPLPLSGASFLQLLQNLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSM 454

  Fly   439 LFPFKWQCPYIPLCPLGLAEVLHAPLPYLIGVDSRFFDLYEPPTDVTCIDLDTNNISLCESQRHL 503
            :||..|||||||||||.||:||.||:|:::|:.|.:|||::||.||.|:|||||.:...|.::.|
  Rat   455 IFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPL 519

  Fly   504 SPKLLPKRAARLLRQTLTELENAKPISYD------STNSLDRDIR----KRKRELVLEQRIQEAF 558
            |.:.||:|..:||..|||.|......:|.      |...|..|..    :|.|   ||:.:|.||
  Rat   520 SARTLPRRPYKLLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRTR---LEREVQGAF 581

  Fly   559 LLFMASILRGYRDFLVPISKAPSVGATDPSALFQLKAFLRSRDKSHQKFFELMMKTQMFIRFIEE 623
            |.|||.:|:|||:||.|:::|||.|:.|...||.|:.||:||::|..|.:..::.||||.:||||
  Rat   582 LRFMACLLKGYRNFLRPLTQAPSEGSRDVENLFYLQGFLKSRERSSHKLYSQLLHTQMFSQFIEE 646

  Fly   624 RSFVSDGDHGLSFFDECAEKVGNYDETPAQLHLVDWDTGQNSERTKYIFPPDS---VTPAGSQPG 685
            .||.|.....|.|||.|.:||....|.|....||:.:....||.|.:|.||:.   :..:.|.|
  Rat   647 CSFGSTRHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVLEGSESTP- 710

  Fly   686 GGGGGLAYNYENFTLQPELLQSTKKTALSKFLQLQLNASLSPG-------SPIARRTKHEIKLSQ 743
                  .|.|:.|   |||     |..|.:..|.|..|...||       ||..||||.|:|::|
  Rat   711 ------QYCYDGF---PEL-----KAELFESPQEQQGALPVPGPSRSAPSSPAPRRTKQEMKVAQ 761

  Fly   744 KMASRCQQHPEAWSKYLLATCYSLYFLILPSMVLDPRHAGKEPEILRAAYDVL-VRASRLKITCD 807
            ::|.:....||.|::.||..||.|:||.||:.|   |.|......|..||.|| ...||..:..|
  Rat   762 RVAQKSATVPELWARCLLGHCYGLWFLCLPAYV---RSAPSRVRALHTAYHVLREMESRKVVLPD 823

  Fly   808 EFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWNR 872
            |.|||::||||..:..|||:||:...|:|:|:..|.:|||:||:.||||:|| ..|...::||.:
  Rat   824 EVCYRVLMQLCSNYGQPVLSVRVMLEMRRAGIVPNTITYGYYNKAVLESKWP-SGTPGGRLRWAK 887

  Fly   873 IKNVVLGAAQFRKAGKQRAASKVNKSLSASQDQNLSTLETVDGQSRTSLASSSGDGGGHGLLDFA 937
            ::|||.||||||:..|.|  .::.:.....|.|.....:.|..|.:      ||.......|:..
  Rat   888 LRNVVWGAAQFRQPLKDR--RRLQQEQQEQQQQQQQQQQQVAEQQK------SGSSQTEPCLERP 944

  Fly   938 AFDRLRNKLGSIVRQTVTGGNNETMGDAVNNTGLLIPGELSAPNTPTYGGDTEILAKALQQQQPR 1002
            :..|      .:.|||...|.:                 |..|::|.
  Rat   945 SPTR------PLQRQTTWAGRS-----------------LREPSSPI------------------ 968

  Fly  1003 KQIMSIGGGDDDDEDEDEDEYTAGSPSTPQKQLEAGADDLEYAGGGDYEADEEDEDEVDEHVAAQ 1067
            .:::..|              :.||....|..:|||...:
  Rat   969 GRLVKSG--------------SLGSARGAQPTVEAGVAHM------------------------- 994

  Fly  1068 RARQRVQSPTKISPR-TPVTQNDPLGALNE-----EESAASATPTQETQQEQQHSQSQIDSSIYS 1126
                 :::...:.|| :||...|  |:|::     ||.|..::|
  Rat   995 -----IEALGVLEPRGSPVPWQD--GSLSDLSLTGEELAPGSSP--------------------- 1031

  Fly  1127 DKPILFRGQRSATFDESTQI--GKSMHRSETMPVASSGVTNSLA----NIGSSLKFTFGRYSPAR 1185
                  .|..||...:||:.  |.|...|:|     ||......    .:|:.|:...   :|:|
  Rat  1032 ------GGSGSALSAQSTEALEGISGRGSKT-----SGCQEEAGTPRKGLGARLQQLL---TPSR 1082

  Fly  1186 LSLKKDLKLPANIIENISSISPSLTGKKSNELI-----QGSLSSIKSAANSLTKKFDEIKGVISA 1245
            .|....:.:|    |....:.|:......:.|:     .||.:|..|:| ||..::|..:..:|:
  Rat  1083 RSSASRISVP----ELPPDLPPAARRSPMDSLLWPRERPGSTASEVSSA-SLGSEWDISESSLSS 1142

  Fly  1246 NSTPTKTNNGHHPHGLHHGHHHPHHHHHHHSQHGNAEQEEHDAAVHEEGKLRRVSSDLDPWGRLS 1310
            .|                                                |||.|.      |||
  Rat  1143 LS------------------------------------------------LRRSSE------RLS 1153

  Fly  1311 ESRKSSYNNLVPLGENSSTGALHMHAFPAVPDNLYSLTSENAADRDCDVLIQLTTCSQCHNCSVL 1375
            :                :.||..       |.:|.               |.:::||.||.|..|
  Rat  1154 D----------------TPGAFQ-------PPSLE---------------ILMSSCSLCHACDSL 1180

  Fly  1376 VYDEEIMSGWTAEDSNLNTTCHACNKLTVPFLSVQI--ERQVEESEQS----------DPLQDG- 1427
            |||||||:||..:||||||||..|....||.||||.  .|....|.:|          .|:..|
  Rat  1181 VYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDSRPSAPSPKSALAGAGGCKDAPVPGGP 1245

  Fly  1428 --------------KEQIANGNSHKTSLTV-----PYLNPLVLRKELENILTQEGDIALIKPEFV 1473
                          :.|:.||:....|..|     .||:|||||||||:::..||...|..||..
  Rat  1246 GPVLSDRRLCLALDEPQLCNGHMGSASRRVENGAWAYLSPLVLRKELESLVENEGIEVLALPELP 1310

  Fly  1474 DEHPIIYWNLLWLMERIESKTHLPELCL-----PVPSDKEH-------IDPLSKVKTVHIQCLWD 1526
            ..||||:|||||..:|:...:.||.|.|     |.||....       .||.|    ||::.|||
  Rat  1311 AAHPIIFWNLLWYFQRLRLPSILPGLVLASCNGPPPSQLSQGPSSWLTPDPAS----VHVRLLWD 1371

  Fly  1527 NLSLHTEASGPPMYLLYRETQPTSPLIKALLTDQAQLNKNVIQQII------SAIRCNDFATPLK 1585
            .| :....|.||:|:|:|                  ::..:.|:::      |.:......:.|:
  Rat  1372 VL-IPDPNSCPPLYVLWR------------------VHSQIPQRVVWPGPVPSCLSLALLESVLR 1417

  Fly  1586 RLA-NERHKL----------KSNGVERSHSFYRDILFLALTAIGRSNVDLATFHREYAAVFDKLT 1639
            ... ||.||.          ...|:......||:||||.:.|:|:.:||:..|.::|.:.|:||.
  Rat  1418 HFGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAALGKDHVDIVAFDKKYKSAFNKLA 1482

  Fly  1640 ---------ERECNMYYRNQDLPPSASTIFCRAYF 1665
                     :|...|        ||...|.||..|
  Rat  1483 SSMGKEELRQRRAQM--------PSPKAIDCRKCF 1509

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Crag	NP_525080.3	uDENN	189..279	CDD:214824	44/89 (49%)
		DENN	308..492	CDD:460461	94/188 (50%)
		dDENN	551..624	CDD:129037	36/72 (50%)
		PPR repeat	808..837	CDD:276811	14/28 (50%)
Dennd4b	XP_008759470.1	uDENN	209..290	CDD:214824	43/88 (49%)
		DENN	324..508	CDD:460461	94/188 (50%)
		dDENN	574..647	CDD:129037	36/72 (50%)
		PPR_2	822..862	CDD:463778	18/39 (46%)
		PPR repeat	824..853	CDD:276811	14/28 (50%)