DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Crag and denn-4

DIOPT Version :9

Sequence 1:	NP_001162710.2	Gene:	Crag / 31800	FlyBaseID:	FBgn0025864	Length:	1671	Species:	Drosophila melanogaster
Sequence 2:	NP_001294387.1	Gene:	denn-4 / 186100	WormBaseID:	WBGene00018681	Length:	1548	Species:	Caenorhabditis elegans

Alignment Length:	1787	Identity:	557/1787 - (31%)
Similarity:	854/1787 - (47%)	Gaps:	373/1787 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 EEKRIADYFVVAGMPENPQLLQENSFNDSGRLRAATTIEPITDIGVYFPLLGEEVPEGYEILSHT 66
            :|:|:.::||:||:.||..|.:....::...||....:.|||||.|.||.|||:.|:|::::..|
Worm    12 DERRLFEHFVIAGLAENQPLERLKPSSEECGLRNTQPLAPITDIAVIFPSLGEKPPDGFDVIEST 76

  Fly    67 PTGLQANLNHGSVRTTDCYIYFRRGKDRPPLVDIGVLYDGH-ERIMSDAEIVAETPGGRVANVNN 130
            ..|..|:|||||:.....::.::||.::.||||||:|.:|. ::.|.|:.:|..||.||.|:|||
Worm    77 TLGYPADLNHGSIGQKSVFLCYKRGYNKLPLVDIGILDEGRGDKPMIDSNVVQTTPFGRCASVNN 141

  Fly   131 SSAKTFLTYRRARADMPCNELVVTELCVIVQSKGERAPHAFCLIYKTLNKGYVGSDVYLCYKKSM 195
            :|...:||||||..:...::.|||::.||:.:|||..||.:..|.|.||||.|||||::|||||.
Worm   142 ASQGIYLTYRRAPPNSSQSQFVVTDIKVILANKGEIPPHTYYKITKNLNKGLVGSDVFICYKKSQ 206

  Fly   196 YRPKHISYKPEILLRYPTFDHT----DFPLNLCPSVPLFCLPMGASLEAWPHVNGTEKRKPISPV 256
            ...|.::|||.:|..:|..||:    ||  .|..:|.:|||||||.:|.||     .|..|....
Worm   207 GTAKRLAYKPAVLDYFPRSDHSGEMEDF--KLAQNVAMFCLPMGALIECWP-----TKCAPPDRS 264

  Fly   257 FSTFVLTVSDGTYKVYGSALTFYEDYDESKLSAEQKELL-------GWDEEFGAQHS-------- 306
            |||||||..:|| |.||||:||||.|.: :||.:|.|.|       |..|:.|:.:.
Worm   265 FSTFVLTDENGT-KFYGSAVTFYEKYTK-QLSEDQLEQLDLKSDEGGTVEDSGSNNDPADDLQIL 327

  Fly   307 LHMIKAICLLSHHPFGDTFDKWLKYLHRM-VLYDVNIPIPVERYITQLLDEVPFPA---PSIHLQ 367
            .:...:|||:|..||.::|.::|.:|||| .....:.|:|:||||:.|:.||.||.   |.:.:|
Worm   328 FYKNVSICLISRFPFFNSFKRFLFFLHRMSSTPSCSHPVPIERYISHLMYEVSFPTPRRPRVFMQ 392

  Fly   368 LSSESNDRILLTQPEDSPLPRSGAGFHILLQNLGTDNCLHVLLLALTEQKILIHSLRPATLTAVA 432
            |.:|:   |.....:||.||.:||.....|:.||::|.::::||||.|||||||||||..|.|||
Worm   393 LGAEN---ISFDSHDDSQLPLNGAQLIDTLKYLGSENLMYLMLLALLEQKILIHSLRPWMLAAVA 454

  Fly   433 EAIVSLLFPFKWQCPYIPLCPLGLAEVLHAPLPYLIGVDSRFFDLYE-PPTDVTCIDLDTNNISL 496
            |::.:|:|||.|||||||.||||||.|||||||::.|||||:|:||| ||.||||.||||:.||.
Worm   455 ESVCALMFPFHWQCPYIPQCPLGLAGVLHAPLPFIAGVDSRYFELYEDPPDDVTCFDLDTSTISF 519

  Fly   497 CESQRHLSPKLLPKRAARLLRQTLTELENAKPISYDSTNS--------LDRDIRKRKRELVLEQR 553
            ...:......:|||:.|:.|:..|.::.........||||        :|:|:.|:::....|..
Worm   520 STVRATFKTSMLPKKPAKQLKSALEDIFGKLQKQDYSTNSKSDGGFIPVDQDLVKQRQRREFENE 584

  Fly   554 IQEAFLLFMASILRGYRDFLVPISKAP-SVGATDPSALFQLKAFLRSRDKSHQKFFELMMKTQMF 617
            |.:|||.|:|||::||:.||.||..|| :..|||...||.|..||||||:|...|::...:||.|
Worm   585 IHDAFLKFIASIMKGYQAFLRPIKGAPMTEHATDTGNLFDLDGFLRSRDRSSADFYKRFSETQSF 649

  Fly   618 IRFIEERSFVSDGDHGLSFFDECAEKVGNYDETPAQL---HLVDWDTGQN----------SERTK 669
            :|||||||||||.:...:|||:|..|:.:..|....:   .|::.||..:          ..||.
Worm   650 MRFIEERSFVSDKNTYNAFFDDCIAKLTSAVEDGRDISGVQLLEPDTSHSHTTSTINALADTRTS 714

  Fly   670 YIFPPDSVTPAGSQPGGGGGGLAYNYENFTLQPELLQSTKKTALSKFLQL-QLNASLSPGSPI-- 731
            .:|.| :..|..:|.  .|..|.:.|..|   |..::       |::.|| :|..:...|..:
Worm   715 MVFIP-APEPFLNQE--TGEELQFVYGEF---PSKMR-------SEYFQLDRLEKNGKDGEKVPM 766

  Fly   732 ----------ARRTKHEIKLSQKMASRC-QQHPEAWSKYLLATCYSLYFLILPSMVLDPRHAGKE 785
                      |.|||.|.:.|...|:.. :.:|..|||.||...|||:|:.|.|::   ..|..:
Worm   767 HFVSEASRCSAVRTKPETRSSLLAATNAVKTNPLHWSKTLLFYSYSLWFMQLDSLL---TAAPNK 828

  Fly   786 PEILRAAYDVLVRASRLKI-TCDEFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFY 849
            .:|||.|::||.|..:.:| ..|:.||||:::|||.::.|.:||::...|:|:|::.||:|||.|
Worm   829 KKILRLAFNVLDRMEKTEIFPLDQVCYRILIELCGKYSEPEMAVKVMRAMQRAGLEQNAVTYGIY 893

  Fly   850 NRCVLESQWPQDSTTISQIRWNRIKNVVLGAAQFRK-AGKQRAASKVN-KSLSASQD-QNLSTLE 911
            :|.|:::.||..:...:...||.::.|:.||...|| |..:|...|:: ||:..::: :|:....
Worm   894 HRAVMDATWPSPARQKAIKMWNLLRIVLTGAETLRKWANLERRPLKIDEKSVENTENAENVQAEI 958

  Fly   912 TVDGQSRTSLASSSGDGGGHGLLDFAAFDRLRNKLGSIVRQTVTGGNNETMGDAVNNTGLLIPGE 976
            |||.|. ||...                                        |:|.|:..:.|..
Worm   959 TVDYQI-TSYPL----------------------------------------DSVENSPKMTPKR 982

  Fly   977 LSAPNTPTYGGDTEILAKALQQQQPRKQIMSIGGGDDDDEDEDEDEYTAGSPSTPQKQLEAGADD 1041
            .:                 |:|::|   :|              |...|..|      ||  |::
Worm   983 HN-----------------LEQKEP---LM--------------DPLGALGP------LE--AEN 1005

  Fly  1042 LEYAGGGDYEADEEDEDEVDEHVAAQRARQRVQSPTKI--SPRTP--VTQNDPLGALNEEESAAS 1102
            ||                    |:.::.::|.:|..|:  ||...  ...:..|...||.
Worm  1006 LE--------------------VSREKTKERSKSLGKVGMSPARAKFFADHATLPFSNEN----- 1045

  Fly  1103 ATPTQETQQEQQHSQSQIDSSIYSDKPILFRGQRSATFDESTQIGKSMHRSETMPVASSGVTNSL 1167
             ||..:.::.:......|.:|     | :|:..:|.|||.|.| ..:...|||..:  :|.:.|.
Worm  1046 -TPKSDKKEAKSGWFKGIANS-----P-MFKMIKSTTFDSSKQ-NSAESSSETSSI--TGASPSF 1100

  Fly  1168 ANIGSSLKFTFGRYSPARLSLKKDLKLPANIIENISSISPSLTGKKSNELIQGSLSSIKSAANSL 1232
            .::.:..|..:....|:|..|            .:|::.....|...:.:|   .|..:::|.:
Worm  1101 HSVVNQFKKGYDDVMPSRFRL------------GVSTLLNKSVGAMGSGMI---YSDDRASAEN- 1149

  Fly  1233 TKKFDEIKGVISANSTPTKTNNGHHPHGLHHGHHHPHHHHHHHSQHGNAEQEEHDAAVHEEGKLR 1297
                            |...|.....:.|..|                    ..:..:.||..:|
Worm  1150 ----------------PFFVNPKDPAYVLDSG--------------------VPNCLLREEYWMR 1178

  Fly  1298 RVSSDLD-PWGRLSESRKSSYNNLVPLGENSSTGALHMHAFPA-VPDNLYSLTSENAADRDCDVL 1360
            .|...|. ...|:.:.:||..|      |..|.|......|.| .|..|             :|:
Worm  1179 EVYLQLKRSRERVEKGQKSEEN------EEISDGIADFPQFSAKTPGKL-------------EVI 1224

  Fly  1361 IQLTTCSQCHNCSVLVYDEEIMSGWTAEDSNLNTTCHACN------------------KLTVPFL 1407
              |.|.:.|.:|..:|||||||:||..:|.||||.|..|:                  .:..|.|
Worm  1225 --LCTATYCPSCRTMVYDEEIMAGWKVDDQNLNTICPHCSGSGTATDAVSNETAAIQESVFAPRL 1287

  Fly  1408 SVQIERQVEESEQSDPLQDGKEQIANGNSHKTS-------LTVPYLNPLVLRKELENILTQEGDI 1465
            .::: |.:||.:.|.....|.::.......||:       |.|.:::|||||:|||.:|:.:.|
Worm  1288 KIRM-RWIEEMKSSWYQPGGLQETPISTPEKTTSTPSDIQLEVAFVSPLVLRRELETLLSTDKD- 1350

  Fly  1466 ALIKPEFVDEHPIIYWNLLWLMERIESKTHLPELCLPVPSDKEHIDPLSKVKTVHIQCLWDNLSL 1530
            |:........||:::|||::.:.|:...:||.....     :.|          ||:|::|..:.
Worm  1351 AMRNESLKTTHPVVFWNLIYYLRRLSLPSHLFSWIA-----ERH----------HIRCVFDLPTQ 1400

  Fly  1531 HTEASGPPMYLLYRETQPTSP--------LIKAL--------------LTDQAQLNKNVIQQIIS 1573
            |.| :..|:|.........:|        :.:.|              :||  .:|||::.:.:
Worm  1401 HDE-TRLPLYFYNPNHDGNAPSFGDFTEKITRKLEDGSDSTGYATWKTVTD--SVNKNLLFKAV- 1461

  Fly  1574 AIRCNDFATPLKRLANERHKLKSNGVERSHSF--YRDILFLALTAIGRSNVDLATFHREYAAVFD 1636
                       :.|.|....:|:..|.....|  :|||.|.::...||:.:..| ...:|.:..:
Worm  1462 -----------QTLVNSSRLVKNGSVHVGAHFPIFRDIQFASIDVFGRALLRDA-LDTQYDSESN 1514

  Fly  1637 KLTERECNMYYRNQDLPPSASTIFCRAYFRPL 1668
            ||..: .......||.|.|..|..||..|.||
Worm  1515 KLPPK-IKEILPLQDHPLSHVTRACRKVFMPL 1545

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Crag	NP_001162710.2	uDENN	189..279	CDD:214824	44/93 (47%)
		DENN	308..492	CDD:307995	100/188 (53%)
		dDENN	551..624	CDD:129037	38/73 (52%)
		PPR repeat	808..837	CDD:276811	11/28 (39%)
denn-4	NP_001294387.1	DUF2464	51..>205	CDD:287242	73/153 (48%)
		uDENN	199..286	CDD:214824	44/94 (47%)
		DENN	329..514	CDD:280329	99/187 (53%)
		dDENN	582..656	CDD:129037	38/73 (52%)
		PPR_2	851..892	CDD:289786	18/40 (45%)
		PPR repeat	852..881	CDD:276811	11/28 (39%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160159429
Domainoid	1	1.000	203	1.000	Domainoid score	I1739
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	711	1.000	Inparanoid score	I337
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46628
OrthoDB	1	1.010	-	-		D47872at33208
OrthoFinder	1	1.000	-	-		FOG0001463
OrthoInspector	1	1.000	-	-		oto17976
orthoMCL	1	0.900	-	-		OOG6_102394
Panther	1	1.100	-	-	LDO	PTHR12296
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X917
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.870