DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Crag and dennd4b

DIOPT Version :9

Sequence 1:	NP_001162710.2	Gene:	Crag / 31800	FlyBaseID:	FBgn0025864	Length:	1671	Species:	Drosophila melanogaster
Sequence 2:	XP_031746823.1	Gene:	dennd4b / 100125065	XenbaseID:	XB-GENE-5886552	Length:	1503	Species:	Xenopus tropicalis

Alignment Length:	1776	Identity:	550/1776 - (30%)
Similarity:	842/1776 - (47%)	Gaps:	390/1776 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MEE--KRIADYFVVAGM-PENPQLLQENSFNDSGRLRAATTIEPITDIGVYFPLLGEEVPEGYEI 62
            |||  .::.|||||||: |.:..|.:||      |.|:..|.|.:||:.|.....|||||:|:..
Frog     1 MEEGLPKLVDYFVVAGLTPASRPLEEEN------RQRSIRTTELVTDVAVIVKAQGEEVPQGFTC 59

  Fly    63 LSHTPTGLQANLNHGSVRTTDCYIYFRRGKDRPPLVDIGVLYDGHERIMSDAEIVAETPGGRVAN 127
            :..||.|..|:||.|.:.....|:.:|||:|:||:.::||.|||.|.:....:::..||....||
Frog    60 IETTPGGHSADLNSGLLTNQQMYLCYRRGRDKPPITELGVHYDGKEPLRPGFQVIDTTPYSHSAN 124

  Fly   128 VNN---SSAKTFLTYRRARADMPCNELVVTELCVIVQSKGERAPHAFCLIYKTLNKGYVGSDVYL 189
            :::   .:.:.||.::||...|..|.|.|.::|:|..||||..|:.||.:.:.||....|..::|
Frog   125 LSSGGPGNQRAFLMFKRAPESMGLNSLGVMDICIINPSKGESTPNTFCRVDRNLNTSMFGPALFL 189

  Fly   190 CYKKSMYRPKHISYKPEILLRYPTFDHTDFPLNLCPS-VPLFCLPMGASLEAWPHVNGTEKRKPI 253
            ||||...:...:.|:..:|.|:|:.|...|||   |. |..|||||||::|:|| :|    .|..
Frog   190 CYKKGTAKTHSLVYEAGVLSRFPSADSETFPL---PEMVATFCLPMGATIESWP-IN----TKYH 246

  Fly   254 SPVFSTFVLTVSDGTYKVYGSALTFYEDYDESKLSAEQKELLGW----DEEFGAQHSLHMIKAIC 314
            .|||||||||.:.|. ||||:|:.|:|.|....||.:|.:.||.    |:......|:...|:||
Frog   247 MPVFSTFVLTGASGE-KVYGAAIQFHEQYPRGCLSEKQNQSLGLLSVVDKRPVTNKSVQTKKSIC 310

  Fly   315 LLSHHPFGDTFDKWLKYLHRMVLYDVNIP--IPVERYITQLLDEVPFPAPS---IHLQLSSESND 374
            :|||.||.|.|.|:|.:::|   |.::.|  :|:|::|:..:..||||:|.   |.:|:|  ..|
Frog   311 VLSHWPFFDVFQKFLTFIYR---YSISGPHVLPIEKHISNFMFNVPFPSPQRPRILVQMS--PYD 370

  Fly   375 RILLTQPEDSPLPRSGAGFHILLQNLGTDNCLHVLLLALTEQKILIHSLRPATLTAVAEAIVSLL 439
            .:||.||..||||.|||.:..||||||.:..:.:||..|||.|:||||.||..||:|.||:||::
Frog   371 NVLLCQPVSSPLPLSGASYVTLLQNLGPETAVTLLLAVLTEHKLLIHSQRPDVLTSVGEALVSII 435

  Fly   440 FPFKWQCPYIPLCPLGLAEVLHAPLPYLIGVDSRFFDLYEPPTDVTCIDLDTNNISLCESQRHLS 504
            ||.:|||||||||||.|::||.||:|:::|:.|.:|||::||.||.|:|||||.:...|.::..|
Frog   436 FPLRWQCPYIPLCPLTLSDVLCAPVPFIVGIHSSYFDLHDPPQDVLCVDLDTNTLFQSEEKKVPS 500

  Fly   505 PKLLPKRAARLLRQTLTELENA------KPISYDSTNSLDRD---IRKRKRELVLEQRIQEAFLL 560
            .:.||::..::|..||::|...      ||:...:...|.:|   |..|:.:|.|:  ::.|||.
Frog   501 VRSLPRKPCKVLLGTLSDLHKQLDEMYNKPMEEATLEFLLKDYDLIYGRRTQLELD--VRAAFLR 563

  Fly   561 FMASILRGYRDFLVPISKAPSVGATDPSALFQLKAFLRSRDKSHQKFFELMMKTQMFIRFIEERS 625
            |||.:|:|||.||:||::|||....|.|.||.::.|.:|||::||||:..|:|||||.:|||:.|
Frog   564 FMACLLKGYRSFLLPITQAPSERTRDSSNLFNVEGFRKSRDRAHQKFYSQMLKTQMFTQFIEDCS 628

  Fly   626 FVSDGDHGLSFFDECAEKVGNYDETPAQLHLVDWDTGQNSERTKYIFPPDSVTPAGSQPGGGGGG 690
            ||||....|.|.|.|.|||....|....||||:.|....||.|.:|.|.:.    ..:||.|...
Frog   629 FVSDRHACLEFLDNCIEKVQIDGEKMEDLHLVELDESHRSEHTVFIMPAEE----PHEPGSGELP 689

  Fly   691 LAYNYENF-TLQPELLQSTKKTALSKFLQLQLNASLSPGSPIARRTKHEIKLSQKMASRCQQHPE 754
            ..|.||:| .|:.||....:...::...|   :.|.:|.||..||||.|||::|::|.:....|:
Frog   690 AQYMYESFPVLKWELFDLPQDLLMAPPNQ---SKSSAPSSPAPRRTKQEIKMAQRVAQKYSAMPD 751

  Fly   755 AWSKYLLATCYSLYFLILPSMVLDPRHAGKEPEILRAAYDVLVRASRLKITC-DEFCYRIMMQLC 818
            .|:|.||..||.|:|:.||:.|   |....:...|:.|||.|.:....|:.. ||.|||::||||
Frog   752 MWAKCLLGHCYGLWFIYLPTFV---RATPSKVRALQTAYDALKQMENKKVVLPDEVCYRVLMQLC 813

  Fly   819 GIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWNRIKNVVLGAAQF 883
            |.:..||||||:...||::|:..|.:|||:||:.:|||:||..|.: .::||.:::|||||||||
Frog   814 GQYGEPVLAVRVMLEMKKAGIVPNIITYGYYNKAILESKWPSSSQS-GRLRWAKLRNVVLGAAQF 877

  Fly   884 RKAGKQRAASKVNKSLSASQDQN-------------LSTLE--TVDGQSRTSLASSSGDGGGHGL 933
            |:..|::   :....||.||.::             ||.|.  |..|.|......|..
Frog   878 RQPLKRK---RRQAGLSLSQTESHLPRNCASELPPRLSLLRQATWAGSSHREFHESCA------- 932

  Fly   934 LDFAAFDRLRNKLGSIVRQ-TVTGGNNETMGDAVNNTGLLIPGELSAPNTPTYGGDTEILAKALQ 997
                         |.:|:. :|:|.                            |.|.:::..:|.
Frog   933 -------------GKLVKSWSVSGA----------------------------GSDGDMVESSLA 956

  Fly   998 QQQPRKQIMSIGGGDDDDEDEDEDEYTAGSPSTPQKQLEAGADDLEYAGGGDYEADEEDEDEVDE 1062
            ......:.|.:|...:            ||||
Frog   957 HMMEDAEGMKMGRRGE------------GSPS--------------------------------- 976

  Fly  1063 HVAAQRARQRVQSPTKISPRTPVTQNDPLGALNEEESAASATPTQETQQEQQHSQSQIDSSIYSD 1127
                  .|:...:|..:.|    ::|..|..:: .::.|:.:....|.::.:...::..:.....
Frog   977 ------LRRPCGTPLALGP----SENGSLSNVS-LQTDATGSDVAVTSEDSEALMTESSTEALPC 1030

  Fly  1128 KPILFRGQRSATFDESTQIGKSMHRSETMPVASSGVTNSLANIGSSLKFTFGRYSPARLSLKKDL 1192
            :|....|..|:|                   .::|..:|||  |...:.......|.|::|   |
Frog  1031 RPARDLGDSSST-------------------GTNGKRHSLA--GKIQQLLSPARKPGRVTL---L 1071

  Fly  1193 KLPANIIENISSISPSLTGKKSNELIQGSLSSIKSAANSLTKKFDEIKGVISANSTPTKTNNGHH 1257
            :..:::.|..|.:  |..|.:|....:.|:...:|.|:.::|....:...:..:.|...:...|.
Frog  1072 RKKSSMSEKPSPL--SFPGDQSWRSYKESIGRPESTASEVSKSSVSLGSEVDLSDTSVCSFTLHK 1134

  Fly  1258 PHGLHHGHHHPHHHHHHHSQHGNAEQEEHDAAVHEEGKLRRVSSDLDPWGRLSESRKSSYNNLVP 1322
                                                      |:|     ||::..:.:     |
Frog  1135 ------------------------------------------STD-----RLTDGGQEA-----P 1147

  Fly  1323 LGENSSTGALHMHAFPAVPDNLYSLTSENAADRDCDVLIQLTTCSQCHNCSVLVYDEEIMSGWTA 1387
            ..|                                   :.|:.||.|..|..|||||:||:|||:
Frog  1148 AAE-----------------------------------VMLSGCSLCPVCHSLVYDEDIMAGWTS 1177

  Fly  1388 EDSNLNTTCHACNKLTVPFLSVQI-ERQVEESEQSD------PLQDGKEQIANG----------- 1434
            :||||.|.|..|.|..||.|:|:: :.|...|...|      |..:|...:..|
Frog  1178 DDSNLKTVCTFCGKYFVPLLNVRVTDLQSRHSASKDSSASPQPSTNGDSPVPGGAGPVLSDRFQC 1242

  Fly  1435 --------------------------NSHKTSLTVPYLNPLVLRKELENILTQEGDIALIKPEFV 1473
                                      ::....:||.||.|||||||||::|..||...|.:||.|
Frog  1243 LVLDDQGTVRPPCNGYTDTQLQLPGPSASSEVVTVAYLCPLVLRKELESLLENEGGDVLSQPELV 1307

  Fly  1474 DEHPIIYWNLLWLMERIESKTHLPELCLPVPSDKEHIDPLS---KVKTVHIQCLWDNLSLHTEAS 1535
            :.||||||||:|..:|:...::|.:|.|.    .:|:...|   :|..|.::.|||.|....| .
Frog  1308 NSHPIIYWNLVWYFKRMALPSNLLQLVLA----SKHVRQQSQVLEVPQVRVRLLWDVLMQDPE-R 1367

  Fly  1536 GPPMYLLYRETQPTSPLIKALLTDQAQLNKN-----------------VIQQIISAIRCNDFATP 1583
            .||:|:|::                  ||:|                 |:::|::.:..|:....
Frog  1368 WPPLYVLWK------------------LNRNLPTHLHSWNHAHPFTLEVLEKILNFVGLNEVHKA 1414

  Fly  1584 LKRLANERHKLKSNGVERSHSFYRDILFLALTAIGRSNVDLATFHREYAAVFDKLT----ERECN 1644
            :..:.....:..|.........||::|.|.|.|:|:.::|:..|..:|...|.||:    :.|..
Frog  1415 ISLIIGILQEQPSPPQSMQRGIYRELLLLTLAALGKDHMDIGAFDNKYKTAFKKLSSSLGKEELR 1479

  Fly  1645 MYYRNQDLPPSASTIFCRAYF 1665
               |.:...|:...:.||..|
Frog  1480 ---RRRAQFPTPKALDCRRTF 1497

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Crag	NP_001162710.2	uDENN	189..279	CDD:214824	42/90 (47%)
		DENN	308..492	CDD:307995	95/188 (51%)
		dDENN	551..624	CDD:129037	38/72 (53%)
		PPR repeat	808..837	CDD:276811	17/28 (61%)
dennd4b	XP_031746823.1	uDENN	207..268	CDD:397495	35/69 (51%)
		DENN	307..488	CDD:396629	95/185 (51%)
		dDENN	557..627	CDD:129037	38/69 (55%)
		PPR_2	801..841	CDD:404055	20/39 (51%)
		PPR repeat	803..832	CDD:276811	17/28 (61%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	218	1.000	Domainoid score	I2601
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	976	1.000	Inparanoid score	I316
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D47872at33208
OrthoFinder	1	1.000	-	-		FOG0001463
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X917
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	6	6.060