DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG32305 and Adcy8

DIOPT Version :9

Sequence 1:	NP_728725.2	Gene:	CG32305 / 317967	FlyBaseID:	FBgn0052305	Length:	1164	Species:	Drosophila melanogaster
Sequence 2:	NP_058838.1	Gene:	Adcy8 / 29241	RGDID:	2036	Length:	1248	Species:	Rattus norvegicus

Alignment Length:	1160	Identity:	305/1160 - (26%)
Similarity:	525/1160 - (45%)	Gaps:	241/1160 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    29 LKNKCQNLRLELACSRL-----RELALMSNVVQIIWHVELLMVAHVILLLALVKDI--ILVTMVP 86
            |:|..::..||....|.     |:..::.||:.::..:.|| |.|:.|..|.:..:  ||:....
  Rat   154 LRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLL-VLHLSLASAPMDPLKGILLGFFT 217

  Fly    87 YFAVMLFSPFIMLLSMQNNVGMITNTLISWLMALFLTSMDLVSDL-LGTIYSGAAYPTVPCYDHV 150
            ...|::.:..::.....::..:..:.:::|:.   :|:..|.:.| .|.:..|..|
  Rat   218 GIEVVICALVVVRKDTTSHTYLQYSGVVTWVA---MTTQILAAGLGYGLLGDGIGY--------- 270

  Fly   151 VLVSVYMT-----LPIAFYQGSSVYVLGMVVSIIYIGYAFYMQYLNDQVSFY----LLASYAIYL 206
            ||.:::.|     ||:.:     ..:.|:..|::.:    .:|.|..:::.:    :||...:::
  Rat   271 VLFTLFATYSMLPLPLTW-----AILAGLGTSLLQV----TLQVLIPRLAVFSINQVLAQVVLFM 326

  Fly   207 FTLNLIFSFFTIIRDYGLRRAILSRYQLVYQNIVYQLVMKKENGLLESILPRKMIRTLQEEICSR 271
             .:|....|.:.:.|...|:|.|...:.|...:..:...:::..|:.|:|||.::..:..:: :.
  Rat   327 -CMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDM-TN 389

  Fly   272 IEDQDKNFTPSKAGLRKLFLEPYPNVSILVADMVNYTHLTTTLEAPQLVEILHDLFVNFDLAANR 336
            :||:.....     ..::::..|.|||||.||:..:|:|:|||.|.:||.:|::||..||..|:.
  Rat   390 VEDEHLQHQ-----FHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHE 449

  Fly   337 NRAMRIKFLGDSYNCVAGIPNYFPAHASCCVDQALEMIHITQGVSSRRELDINLRIGVHSGEVFA 401
            :..:|||.|||.|.||:|:|.....||.|||:..|.||...:.|.||.:.|:::|||:|||.|..
  Rat   450 HHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLC 514

  Fly   402 GIIGHTKWQFDIWSKDVDITNRLESSGLPGLVHVSQRTLSMLDEHYIFREGTEAAKNDPILQQAG 466
            |::|..|||||:||.||||.|:|||.|:||.:|:|:.||..|...|...||....:|: .|::..
  Rat   515 GVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDYNVEEGHGKERNE-FLRKHN 578

  Fly   467 IRTFLVSN------RLPDAV-----------------EPGELDDELSSASI------------NS 496
            |.|:|:..      .||:.:                 ..|....||...:|            ||
  Rat   579 IETYLIKQPEESLLSLPEDIVKESVSCSDRRNSGATFTEGSWSPELPFDNIVGKQNTLAALTRNS 643

  Fly   497 CRLSYGGYYEEIQIKA-QREIMKEVDQMAVGHCFIEWRRSSSMKKQDFNEEYLFSNQIHLVLGVF 560
            ..|......:.:.::: ..||.|.::..      |:.|....::::......|          :|
  Rat   644 INLLPNHLAQALHVQSGPEEINKRIEHT------IDLRSGDKLRREHIKPFSL----------MF 692

  Fly   561 RSWKREWEFHNLPDLMIKYTMLLVLCAGLAIMSINLIEEATYP-----------DILVLLGILLI 614
            :....|.::..:.|.:.|..:   :||.:.::.|..| ::..|           .||::|...|:
  Rat   693 KDSSLEHKYSQMRDEVFKSNL---VCAFIVLLFITAI-QSLLPSSRLMPMTIQFSILIMLHSALV 753

  Fly   615 LLILCILAGYKKLRLQWKKLTPMSQPSCFLSRWLLRISERIEDSLFVRIPLTMVVLLLLYVMSSQ 679
            |:...  ..||.|.|..:|       :|.   |       |.::...|..:....:|:.::.:..
  Rat   754 LITTA--EDYKCLPLILRK-------TCC---W-------INETYLARNVIIFASILINFLGAVI 799

  Fly   680 AVFSCDVAKLELSIIEAHL-YNEK-------SYAECF---GPWTVTYCVVIVISLLFVVPGCPLV 733
            .:..||..|   ||...:| :|..       ||.|.|   |...:..|.|.:        ....|
  Rat   800 NILWCDFDK---SIPLKNLTFNSSAVFTDICSYPEYFVFTGVLAMVTCAVFL--------RLNSV 853

  Fly   734 IKILAGLLILGLH-LGTVHLYYGFAFERSETTDLGLKSDYAHT----WYLVALFIVLVL-RERHV 792
            :|:...|:::.:: |.|..:|.|...........|  .|:..|    ..|:|:|::.|. ..:.:
  Rat   854 LKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSG--EDFLGTKEASLLLMAMFLLAVFYHGQQL 916

  Fly   793 NYLRKFNYFMRV-CYEEAHQQTDEKLRSIKIIMANILPTHVAQVYKVR-RPHDQLYYENFSKVAV 855
            .|..:.::..|| ..||.::..|.:..: :.::.||||.|||:.:..: |.:::||.:::..|.|
  Rat   917 EYTARLDFLWRVQAKEEINEMKDLREHN-ENMLRNILPGHVARHFLEKDRDNEELYSQSYDAVGV 980

  Fly   856 MFASIENF----------NADTAGLRILHEIICCFDDLLVDYQTRYK-IEKIKVMGWTYMVACGL 909
            |||||..|          |.....||:|:|||..||:||  .:.|:: |||||.:|.|||...||
  Rat   981 MFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELL--GEDRFQDIEKIKTIGSTYMAVSGL 1043

  Fly   910 ETDHYTDFSIDIPVKQPEADSEIRRGSSVLTVHFGSTEDDEMSGDNVSQPYAQVQDV--AVLVMT 972
            .           |.||                                    |.:|.  .:..:.
  Rat  1044 S-----------PEKQ------------------------------------QCEDKWGHLCALA 1061

  Fly   973 EFALDLLRIMHDIRYNYVFSEYDTFLTGSLKIGISHGSVMAGVVGLSKPHYDIWGHTVNMASRMS 1037
            :|:|.|...:.:|. .:.|:.::      |:||||||||:|||:|..||.|||||.|||:||||.
  Rat  1062 DFSLALTESIQEIN-KHSFNNFE------LRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMD 1119

  Fly  1038 STGLLDNIQVTRHTAKVLRQFNIRCNYRGHTEVKGV----GKVPTYLVV--VDPD 1086
            |||:...|||...|..:|:......:|||...|||:    ||:.||.::  |.|:
  Rat  1120 STGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPN 1174

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG32305	NP_728725.2	CYCc	251..449	CDD:214485	85/197 (43%)
		Nucleotidyl_cyc_III	292..445	CDD:299850	77/152 (51%)
		CYCc	814..1056	CDD:214485	86/255 (34%)
		Nucleotidyl_cyc_III	845..1081	CDD:299850	88/252 (35%)
Adcy8	NP_058838.1	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000269\|PubMed:16258073, ECO:0000269\|PubMed:22494970, ECO:0000269\|PubMed:22976297	1..179		6/24 (25%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000269\|PubMed:22976297	1..106
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	51..117
		AC_N	<158..397	CDD:292831	47/262 (18%)
		CYCc	363..561	CDD:214485	84/203 (41%)
		Guanylate_cyc	402..586	CDD:278633	85/184 (46%)
		DUF1053	614..708	CDD:283888	16/109 (15%)
		CYCc	941..1145	CDD:214485	86/260 (33%)
		Guanylate_cyc	970..1169	CDD:278633	88/254 (35%)
		Involved in CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	1106..1248		31/69 (45%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000269\|PubMed:19305019	1197..1212
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1220..1248
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810