DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nrg and robo2

DIOPT Version :9

Sequence 1:	NP_001245581.1	Gene:	Nrg / 31792	FlyBaseID:	FBgn0264975	Length:	1309	Species:	Drosophila melanogaster
Sequence 2:	NP_001259868.1	Gene:	robo2 / 44522	FlyBaseID:	FBgn0002543	Length:	1519	Species:	Drosophila melanogaster

Alignment Length:	1445	Identity:	308/1445 - (21%)
Similarity:	472/1445 - (32%)	Gaps:	425/1445 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 ILAALLVALLCAGSAESKGNRPPRITKQPAPGELLFKVAQQNKESDNPFIIECEADGQPEPEYSW 71
            :|.|.|..|...|:.:.:.   |||.:.|         .......::||...|:|:|.|.|...|
  Fly    72 LLLAGLNGLTQVGALKGEN---PRIIEHP---------MDTTVPKNDPFTFNCQAEGNPTPTIQW 124

  Fly    72 IKNGKKFDWQAYDNRMLRQPGRGTL---VITIPKDEDRGHYQCFASNEFGTATSNSVYVRKAEL- 132
            .|:|::.......:|:: .|..|..   ||...::.|.|.|.|.|.||||.|.|.:..::.|.|
  Fly   125 FKDGRELKTDTGSHRIM-LPAGGLFFLKVIHSRRESDAGTYWCEAKNEFGVARSRNATLQVAFLR 188

  Fly   133 NAFKDEAAKTLEAVEGEPFMLKCAAPDGFPSPTVNWMIQESIDGSIKSINNSRMTLDPEGNLWFS 197
            :.|:.|.|.| ...:||..:::|.||.|.|.|.::|   .....::..:.|.|:.:...|||...
  Fly   189 DEFRLEPANT-RVAQGEVALMECGAPRGSPEPQISW---RKNGQTLNLVGNKRIRIVDGGNLAIQ 249

  Fly   198 NVTREDASSDFYYACSATSVFRSEYKIGNKVLLDVKQMGVSASQNKHPPVRQYVSR--RQSLALR 260
            ...:.|   |..|.|...:|      :|.:         .||:......||.::.|  :...|:.
  Fly   250 EARQSD---DGRYQCVVKNV------VGTR---------ESATAFLKVHVRPFLIRGPQNQTAVV 296

  Fly   261 GKRMELFCIYGGTPLPQTVWSKDGQRIQWSDRITQGHYGKSLVIRQTNFDDAGTYTCDVSNGVGN 325
            |..:...|..||.|||..:|.:.........|.......:||.:.....:|.|.|||:..|.||.
  Fly   297 GSSVVFQCRIGGDPLPDVLWRRTASGGNMPLRRVHVLEDRSLKLDDVTLEDMGEYTCEADNAVGG 361

  Fly   326 AQSFSIILNVNSVPYFTKEPEIATAAEDEEVVFECRAAGVPEPKISW------------IHNGKP 378
            ..:.. ||.|::.|.|...|:.......:||:|||:|.|.|.|.:.|            ..:|:.
  Fly   362 ITATG-ILTVHAPPKFVIRPKNQLVEIGDEVLFECQANGHPRPTLYWSVEGNSSLLLPGYRDGRM 425

  Fly   379 IEQSTPNPRRTVTDNTIRIINLVKGDTGN-YGCNATNSLGYVYKDVYLNVQAE----PPTISEAP 438
            ....||..|     :.:.|....:.|:|. ..|||.|::|.|.....::|..:    ||.|.:.|
  Fly   426 EVTLTPEGR-----SVLSIARFAREDSGKVVTCNALNAVGSVSSRTVVSVDTQFELPPPIIEQGP 485

  Fly   439 AAVSTVDGRNVTIKCRVNGSPKPLVKWLRASNWLTG--------GRYNVQANGDLEIQDV-TFSD 494
            ...:......|.:.||..|:|.|.|.|     :|.|        .|.|:...|.|.|.|: ...|
  Fly   486 VNQTLPVKSIVVLPCRTLGTPVPQVSW-----YLDGIPIDVQEHERRNLSDAGALTISDLQRHED 545

  Fly   495 AGKYTCYAQNKFGEIQADGSLVVKEHTRITQEPQNYEVAAGQSATFRCNEAHDDTLEIEIDWWKD 559
            .|.|||.|.|:.|:....|.|.:...|    .|......|.:.:|:                  .
  Fly   546 EGLYTCVASNRNGKSSWSGYLRLDTPT----NPNIKFFRAPELSTY------------------P 588

  Fly   560 GQSIDFEAQPRFVKTNDNSLTIAKTMELDSGEYTCVARTRLDEATARANLIVQDVPNAPKLTGIT 624
            |.    ..:|:.|:..:||:|::.|                     |:|          |:.|
  Fly   589 GP----PGKPQMVEKGENSVTLSWT---------------------RSN----------KVGG-- 616

  Fly   625 CQADKAEIHWEQQGDNRSPILHYTIQFNTSFTPASWDAAYEKVPNTDSSFV-VQMSPWANYTFRV 688
                             |.::.|.|:.........|.|...:|.||  :|. ..:.|..||.|.:
  Fly   617 -----------------SSLVGYVIEMFGKNETDGWVAVGTRVQNT--TFTQTGLLPGVNYFFLI 662

  Fly   689 IAFNKIGASPPSAHSDSCTTQP-------DVPFKNPDNVVGQGTEPNNL-VISWTPMPEIEHNAP 745
            .|.|..|.|.||..|:..|...       |:.......:.|...|.:|. |:..|.| ::.....
  Fly   663 RAENSHGLSLPSPMSEPITVGTRYFNSGLDLSEARASLLSGDVVELSNASVVDSTSM-KLTWQII 726

  Fly   746 NFHY----YVSWKRDI-------PAAAWENNN--IFDWRQNNIVIADQPTFVKYLIKVVAINDR- 796
            |..|    || :.|.:       ||....|.|  :.....:....|.....:.....:.|...|
  Fly   727 NGKYVEGFYV-YARQLPNPIVNNPAPVTSNTNPLLGSTSTSASASASASALISTKPNIAAAGKRD 790

  Fly   797 GESNVAAEEVVGYSGEDRPLDAPTNFTMRQITSSTSGYMAWTPVSEESVRGHFKGYKIQTWTENE 861
            ||:|        .||...|  .|.|...|.:|....|..:...::.......::.:.:..:...|
  Fly   791 GETN--------QSGGGAP--TPLNTKYRMLTILNGGGASSCTITGLVQYTLYEFFIVPFYKSVE 845

  Fly   862 GEEGLREIHVKGDTHNALVTQFKPDSKNYARILAYNGRFNGPPSAVIDFDTPEGVPSPV-QGLDA 925
            |                     ||.:...||.|                   |.|||.. .|::|
  Fly   846 G---------------------KPSNSRIARTL-------------------EDVPSEAPYGMEA 870

  Fly   926 YPLGSSAFMLHWKKPLYP--NGKLTGYKIYYEEVKESYVGERREYDPHITDPRVTRMKMAGLKPN 988
            ..|.|||..|.||.|...  :|.|..|.:....:..::...|...:..| |.....:.:|.|...
  Fly   871 LLLNSSAVFLKWKAPELKDRHGVLLNYHVIVRGIDTAHNFSRILTNVTI-DAASPTLVLANLTEG 934

  Fly   989 SKYRISITATTKMGEGSEHYIEKTTLKDAVNVAPATPSFSWEQLPSDNGLAKFRINWLPSTEGHP 1053
            ..|.:.:.|....|.|.  |....||:
  Fly   935 VMYTVGVAAGNNAGVGP--YCVPATLR-------------------------------------- 959

  Fly  1054 GTHFFTMHRIKGETQWIRENEEKNSDYQEVGGLDPETAYEFRVVSVDGHFNTESATQEIDTNTVE 1118
                                            |||.|.      .:|...|......:...|
  Fly   960 --------------------------------LDPITK------RLDPFINQRYPINQDHVN--- 983

  Fly  1119 GPIMVANETVANAGWFIGMMLA-LAFIIILFIIICIIRRNRGGKYDVHDRELANGRR-------- 1174
                    .|....|||.::.| ||.:::.|..:..::|    |:.:..:...|..|
  Fly   984 --------DVLTQPWFIILLGAILAVLMLSFGAMVFVKR----KHMMMKQSALNTMRGNHTSDVL 1036

  Fly  1175 DYPEEGGFHEYSQPLDNKSAGRQSVSSANKPGVESDTDSMAEY-----------GDGDTAVATAA 1228
            ..|.....:.....||:.:.|.....|.....:|...|.:|:|           |.|.::..:..
  Fly  1037 KMPSLSARNGNGYWLDSSTGGMVWRPSPGGDSLEMQKDHIADYAPVCGAPGSPAGGGTSSGGSGG 1101

  Fly  1229 ARAAAAARSKL-----------------TSLPTS------------------------------- 1245
            |.:.|:....:                 :::||.
  Fly  1102 AGSGASGGDDIHGGHGSERNQQRYVGEYSNIPTDYAEVSSFGKAPSEYGRHGNASPAPYATSSIL 1166

  Fly  1246 --TQQQQQQQ---------------------QQQQQQQEQPQSTSASTVPLISLASPSPS----- 1282
              .|||||||                     ||||.||:|.|.|......|.......||
  Fly  1167 SPHQQQQQQQPRYQQRPVPGYGLQRPMHPHYQQQQHQQQQAQQTHQQHQALQQHQQLPPSNIYQQ 1231

  Fly  1283 PSSTSN-SPTAAPPS 1296
            .|:||. .||...||
  Fly  1232 MSTTSEIYPTNTGPS 1246

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nrg	NP_001245581.1	Ig	55..128	CDD:299845	25/75 (33%)
		IG_like	57..127	CDD:214653	24/72 (33%)
		Ig	135..214	CDD:299845	22/78 (28%)
		IG_like	141..216	CDD:214653	19/74 (26%)
		ig	251..332	CDD:278476	21/82 (26%)
		I-set	339..427	CDD:254352	26/100 (26%)
		Ig	354..427	CDD:299845	23/85 (27%)
		I-set	432..517	CDD:254352	29/93 (31%)
		Ig	446..517	CDD:299845	26/79 (33%)
		I-set	522..611	CDD:254352	12/88 (14%)
		ig	525..609	CDD:278476	11/83 (13%)
		FN3	613..707	CDD:238020	22/94 (23%)
		FN3	716..807	CDD:238020	21/105 (20%)
		fn3	817..905	CDD:278470	12/87 (14%)
		FN3	917..1004	CDD:238020	23/89 (26%)
		FN3	1041..1111	CDD:238020	6/69 (9%)
		Bravo_FIGEY	1155..>1221	CDD:290593	14/84 (17%)
robo2	NP_001259868.1	Ig1_Robo	90..185	CDD:143317	30/107 (28%)
		I-set	91..184	CDD:254352	30/102 (29%)
		I-set	190..279	CDD:254352	26/110 (24%)
		Ig2_Robo	193..279	CDD:143201	25/107 (23%)
		I-set	283..370	CDD:254352	23/87 (26%)
		Ig	300..370	CDD:299845	20/70 (29%)
		Ig	388..>457	CDD:299845	20/73 (27%)
		I-set	479..561	CDD:254352	27/86 (31%)
		Ig	496..561	CDD:143165	24/69 (35%)
		FN3	588..681	CDD:238020	31/148 (21%)
		FN3	<817..856	CDD:238020	6/59 (10%)
		fn3	864..952	CDD:278470	22/90 (24%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	P	PTHR12231
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	2	2.010