DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nrg and nfasca

DIOPT Version :9

Sequence 1:	NP_001245581.1	Gene:	Nrg / 31792	FlyBaseID:	FBgn0264975	Length:	1309	Species:	Drosophila melanogaster
Sequence 2:	XP_021335211.1	Gene:	nfasca / 100141490	ZFINID:	ZDB-GENE-080229-6	Length:	1379	Species:	Danio rerio

Alignment Length:	1413	Identity:	416/1413 - (29%)
Similarity:	631/1413 - (44%)	Gaps:	270/1413 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MW--RQSTILAAL-LVALLCAGSA----------ESKGNRPPRITKQPAPGELLFKVAQQNKESD 52
            ||  |:...||.| :::||...:|          :.:..:||.|.||.....::       ...|
Zfish     1 MWTQRRWAALAVLSIISLLSEEAAAIEVPLDPKIQQELKQPPTIVKQSLKDYIV-------DPRD 58

  Fly    53 NPFIIECEADGQPEPEYSWIKNGKKFDWQAYDNRMLRQPGRGTLVI-TIPKDED-RGHYQCFASN 115
            | .||||||.|.|.|.:.|.:|||.|: ...|.|:..:...|||.| :..|.|| .|.|||||||
Zfish    59 N-IIIECEAKGNPVPTFQWRRNGKFFN-VGKDPRVTMRSRSGTLEIRSSGKPEDYEGEYQCFASN 121

  Fly   116 EFGTATSNSVYVRKAELNAFKDEAAKTLEAVEGEPFMLKCAAPDGFPSPTVNWMIQESIDGSIKS 180
            ..|||.||.:.:|.::...:..|..:.:...||.|.:|.|..|.|.|.|...||     |..|..
Zfish   122 NLGTAISNKILLRVSKSPLWPKEVLEPVTVREGSPLVLACNPPPGLPPPETFWM-----DSFIMP 181

  Fly   181 I-NNSRMTLDPEGNLWFSNVTREDASSDFYYACSATSVFRSEYKIGNKVLLDVKQM--------- 235
            | .:.|:::...|:|:||||..:||.:|  |:|:|...|....:..|...|.|:.|
Zfish   182 IAQDRRVSMGLNGDLFFSNVLAKDAKTD--YSCNARFEFTHTIQQKNPYTLKVQTMRNIPETQPT 244

  Fly   236 -----GVSASQNKHPPVRQYVSRRQSLALRGKRMELFCIYGGTPLPQTVWSKDGQRIQWSDRITQ 295
                 |:|:::               :.|||:.:.|.||..|.|.|...|||.|..:. :.::..
Zfish   245 FLSPTGLSSTK---------------MVLRGEELLLECIAAGVPTPTITWSKRGGDLP-TKKVRI 293

  Fly   296 GHYGKSLVIRQTNFDDAGTYTCDVSNGVGNAQSFSIILNVNSVPYFTKEPEIATAAEDEEVVFEC 360
            |::.|:|.|...:.:|.|:|||..||.:|:.: .::.:.|.:.|::.::|.....|.||.....|
Zfish   294 GNFDKTLRILNVSEEDIGSYTCMASNRIGSIR-HTVEVQVKAAPFWLEKPINLVLAPDESGRLVC 357

  Fly   361 RAAGVPEPKISWIHNGKPIEQSTPNPRRTVTDNTIRIINLVKGDTGNYGCNATNSLGYVYKDVYL 425
            ||.|.|:|.|.|:.||:|||.|.|||.|.|.|:||....:....:..|.|||:|..||:..:.::
Zfish   358 RANGSPKPTIQWLVNGEPIESSLPNPNRRVLDDTIIFETVQFESSAVYQCNASNEHGYLLANAFV 422

  Fly   426 NV-QAEPPTISEAPAAVSTVDGRNVTIKCRVNGSPKPLVKWLR--ASNWLTGGRYNVQANGDLEI 487
            :| ...|..:......:..::.....::|...|||.||::|.:  ..:.|.||.|.:..||.|||
Zfish   423 SVLDMAPRMLGPKNQLIKVIENNRTFLECPFFGSPLPLLRWFKNGLGSGLDGGHYRLYHNGTLEI 487

  Fly   488 QDVTFSDAGKYTCYAQNKFGEIQADGSLVVKEHTRITQEPQNYEVAAGQSATFRCNEAHDDTLEI 552
            :.....|.|.|||.|.|..|:::....|.|||.|||.:.|::.....|.:..|.|...||.:|..
Zfish   488 KQARTEDQGTYTCVASNILGKMENQVRLEVKEPTRIVRAPEHKTEPRGTTVRFDCRVKHDPSLPA 552

  Fly   553 EIDWWKDGQSIDFEAQPRFVKTNDNSLTIAKTMELDSGEYTCVARTRLDEATARANLIVQD---- 613
            .:.|.||.:.:.|....| :|.:|.||||......|.|.|||.|:|.:||.||.|.|.|.:
Zfish   553 TVTWLKDEKPLSFSWIGR-LKKDDESLTIYNVNPDDGGTYTCTAKTEIDEDTASARLTVTENDSS 616

  Fly   614 ----------VPNAPK-LTGITCQADKAEIHWEQQGDNRSPILHYTIQFNTS-FTPASWD--AAY 664
                      .|:.|: |......|....:.|....:|.||:.|:.:||... :.|..|.  :.|
Zfish   617 SASNRSASAGRPDPPQDLELSDPSARSVRLTWVPGNENNSPVTHFLVQFEEDRWEPGKWQNLSIY 681

  Fly   665 EKVPNTDSSFVVQMSPWANYTFRVIAFNKIGASP---PSAHSDSCTTQPDVPFKNPDNVVGQGTE 726
            :...|   |..:|:||:.||.|||||.|.:|.|.   |||...:....|||.   |..:.|.|::
Zfish   682 DWNLN---SVNLQLSPFVNYQFRVIAINAVGQSQPSHPSARYQTSGASPDVV---PSGLKGWGSK 740

  Fly   727 PNNLVISWTPMPEIEHNAPNFHYYVSWKRDIPAAAWENNNIFDWRQNNIVIADQPTFVKYLIKVV 791
            .||:.|:|..|...|.|.||..|.:||:|......|  |||...:..: |:.:..|:|.|.||:.
Zfish   741 KNNMEITWQAMQNTERNGPNLRYVLSWRRKDTEEEW--NNITTAKTKH-VVHNTDTYVPYEIKIQ 802

  Fly   792 AINDRG---ESNVAAEEVVGYSGEDRPLDAPTNFTMRQITSSTSGYMAWTPVSEESVRGHFKGYK 853
            |:||.|   |||:    |:||||||:|..|||:..:.:| :||...:.|.||.:.||.|.||.||
Zfish   803 AVNDFGHGPESNM----VIGYSGEDKPSAAPTDLRVSKI-NSTKVNIHWEPVEQSSVNGEFKEYK 862

  Fly   854 IQTWTENEGEEGLR----EIHVKG-----DTHNALVTQFKPDSKNYARILAYNGRFNGPPSAVID 909
            :..|.|....:||:    ::| ||     :..:.::|:..|.||....::..|....||.|..::
Zfish   863 LYYWREASLIKGLKVNKTKLH-KGFFTTAEHPSGILTELYPYSKYKMYMVVANREHEGPQSNTVE 926

  Fly   910 FDTPEGVPSPVQGLDAY---PLGSSAFMLHWKKPLYPNGKLTGYKIYYEEVKESYVGERR--EYD 969
            |.|.||.|    |...|   ...:....|.|.|||.|||.|.||.:.|..|..:..|||:  .:.
Zfish   927 FYTKEGEP----GAPKYFRILRHTDVVHLKWDKPLEPNGVLIGYTLQYHTVNGTQAGERKVQSFP 987

  Fly   970 PHITD------PRVTRMKMAGLKPNSKYRISITATTKMGEGSEHYIEK----------------- 1011
            |::|:      .|.||           ::..:.|.|::|.|..:.:|.
Zfish   988 PNVTEYVMNLPHRFTR-----------FKFFLAARTQVGAGEVYAVESPHFTNEGTPDTDSTVVA 1041

  Fly  1012 ----------------------------------------------------------------- 1011

Zfish  1042 VNFTDGLMFEIPPPPLPPSTDPNPTIFTPEPTPPVPTTAAPTKPTTTTTTTTTTTTTSTTTTTTT 1106

  Fly  1012 ------TTLKDAVNVAPATP----SFSWEQLP-------------SDNGLAKFRINWLPSTEGHP 1053
                  ||...:..|||..|    :..|:.|.             :||....::.|:  |.:...
Zfish  1107 TTTTTTTTTAASTTVAPVRPVPPKTVDWKMLAPNVKIWNLTVDANNDNANVSWKHNF--SVDSSK 1169

  Fly  1054 GTHFFTMHRIKGETQWIRENEEKNSDYQEVGGLDPETAYEFRVVSVDGHFNTESATQEIDTNTVE 1118
            ....||:.. .|....|:.|::   .:.::.||.....|..||.|   |.:...::..:...|
Zfish  1170 FVVEFTLDS-NGSMTSIQVNQQ---PHIKLAGLVAGAKYRLRVYS---HEHNSISSDYVTFET-- 1225

  Fly  1119 GPIMVANETVANAGWFIGMMLALAFIIILFIIICIIRRNRGGKYDVHD-RELANGRRDYPEEGGF 1182
            |......|.:|..|||||:|.|:|.::::.:|:|.|:|:|||||.|.: ::|.....|:.::.|.
Zfish  1226 GRAYKEQEDIATQGWFIGLMCAVALLVLILLIVCFIKRSRGGKYPVREKKDLPLDPVDHKDQDGS 1290

  Fly  1183 HEYSQPLDNKSAGRQSVSSANKP----------GVESDTDSMAEYGDG 1220
            .:|..  :|:|..|:|....|||          .|:...||:.:||:|
Zfish  1291 FDYQN--ENRSLERESSDEDNKPLQGSQTSLDGNVKESDDSLVDYGEG 1336

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nrg	NP_001245581.1	Ig	55..128	CDD:299845	36/74 (49%)
		IG_like	57..127	CDD:214653	35/71 (49%)
		Ig	135..214	CDD:299845	27/79 (34%)
		IG_like	141..216	CDD:214653	27/75 (36%)
		ig	251..332	CDD:278476	25/80 (31%)
		I-set	339..427	CDD:254352	34/87 (39%)
		Ig	354..427	CDD:299845	30/72 (42%)
		I-set	432..517	CDD:254352	25/86 (29%)
		Ig	446..517	CDD:299845	25/72 (35%)
		I-set	522..611	CDD:254352	33/88 (38%)
		ig	525..609	CDD:278476	30/83 (36%)
		FN3	613..707	CDD:238020	32/114 (28%)
		FN3	716..807	CDD:238020	33/93 (35%)
		fn3	817..905	CDD:278470	30/96 (31%)
		FN3	917..1004	CDD:238020	27/97 (28%)
		FN3	1041..1111	CDD:238020	14/69 (20%)
		Bravo_FIGEY	1155..>1221	CDD:290593	24/77 (31%)
nfasca	XP_021335211.1	Ig_hNeurofascin_like	60..134	CDD:143283	36/74 (49%)
		Ig	139..231	CDD:325142	30/98 (31%)
		Ig	263..333	CDD:325142	22/71 (31%)
		Ig	351..425	CDD:325142	30/73 (41%)
		Ig	449..514	CDD:319273	24/64 (38%)
		I-set	522..610	CDD:254352	33/88 (38%)
		FN3	628..717	CDD:238020	29/91 (32%)
		fn3	731..813	CDD:306538	30/84 (36%)
		FN3	828..929	CDD:238020	32/102 (31%)
		FN3	934..1018	CDD:238020	27/98 (28%)
		FN3	1142..1211	CDD:328772	15/77 (19%)
		Bravo_FIGEY	1262..1355	CDD:316405	24/77 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	62	1.000	Domainoid score	I10316
eggNOG	1	0.900	-	-		E1_KOG3513
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	549	1.000	Inparanoid score	I1108
OMA	1	1.010	-	-		QHG47974
OrthoDB	1	1.010	-	-		D208655at33208
OrthoFinder	1	1.000	-	-		FOG0000777
OrthoInspector	1	1.000	-	-		otm24295
orthoMCL	1	0.900	-	-		OOG6_101169
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X532
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	13	12.740