Protein Alignment nAChRalpha3 and Chrna6

DIOPT Version :9

• Sequence 1: NP_001285015.1
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_476532.2
• Gene: Chrna6 / 81721
• RGDID: 69281
• Length: 493
• Species: Rattus norvegicus
• Alignment Length: 758
• Identity: 234/758 - (30%)
• Similarity: 329/758 - (43%)
• Gaps: 290/758 - (38%)

  Fly    14 LSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWV 78
            |:|...|......::|:..|.::||:.:|||.||:|.:||..:|.::||.:|:..||||.||||:
  Rat    22 LAFFKGSRGCVSEEQLFHTLFAHYNRFIRPVENVSDPVTVHFELAITQLANVDEVNQIMETNLWL 86

  Fly    79 EQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPP 143
            ...|.||:|.|:|.||.|:|.|.||:|:||:|||||||||.|:|:|...|||.|.|.|.:.|.||
  Rat    87 RHVWKDYRLCWDPTEYDGIETLRVPADNIWKPDIVLYNNAVGDFQVEGKTKALLKYDGVITWTPP 151

  Fly   144 AIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWD 208
            ||:||||.:|:.:||||.|.|.:||||||||..::||.         ::...||:::|:.:.||:
  Rat   152 AIFKSSCPMDITFFPFDHQNCSLKFGSWTYDKAEIDLL---------IIGSKVDMNDFWENSEWE 207

  Fly   209 ILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKV 273
            |::....:::..|.||:|.|.|||::..:||..:|||:||||||:.|||||:||||||||.||||
  Rat   208 IVDASGYKHDIKYNCCEEIYTDITYSFYIRRLPMFYTINLIIPCLFISFLTVLVFYLPSDCGEKV 272

  Fly   274 SLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTM 338
            :|.||:|||||||.|::.|.||.||||:||:|:::|||||..|.||.|||.|||||:|:|.||||
  Rat   273 TLCISVLLSLTVFLLVITETIPSTSLVIPLVGEYLLFTMIFVTLSIVVTVFVLNIHYRTPATHTM 337

  Fly   339 APWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSP 403
            ..||:|:|:...|..|:|||||....|            .:|::                   .|
  Rat   338 PKWVKTMFLQVFPSILMMRRPLDKTKE------------ADGVK-------------------DP 371

  Fly   404 KT-TSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQ 467
            || |.|.......||.:. .|:.||
  Rat   372 KTHTKRPAKVKFTHRKEP-KLLKEC---------------------------------------- 395

  Fly   468 YHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCS 532
                                                                             
  Rat   396 ----------------------------------------------------------------- 395

  Fly   533 ANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAA 597
                   :|.|
  Rat   396 -------RHCH------------------------------------------------------ 399

  Fly   598 CQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAA 662
             :|||:...::|        .||..|....:.:.|
  Rat   400 -KSSEIAPGKRL--------SQQPAQWVAENSEHP------------------------------ 425

  Fly   663 AGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEES 727
                                                      |::...:|.|.:|.:..:...|:
  Rat   426 ------------------------------------------PDVEDVIDSVQFITENMKSHNET 448

  Fly   728 TRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDT 770
            ..|::||||:|||:||:|||:|.|..|.||.|:.|| |.|.:|
  Rat   449 KEVEDDWKYMAMVVDRVFLWVFIIVCVFGTVGLFLQ-PLLGNT 490

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_001285015.1	Neur_chan_LBD	26..241	CDD:280998	100/214 (47%)
		Neur_chan_memb	248..>359	CDD:280999	73/110 (66%)
		Neur_chan_memb	<683..760	CDD:280999	23/76 (30%)
Chrna6	NP_476532.2	LIC	34..483	CDD:273305	226/736 (31%)
		LGIC_ECD	34..240	CDD:355788	100/214 (47%)
		Cys-loop	158..172	CDD:349787	7/13 (54%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I1995
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H20888
Inparanoid	1	1.050	477	1.000	Inparanoid score	I1422
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm12327
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.870