Protein Alignment nAChRalpha3 and Chrna9

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_075219.2
• Gene: Chrna9 / 65024
• RGDID: 621534
• Length: 479
• Species: Rattus norvegicus
• Alignment Length: 469
• Identity: 179/469 - (38%)
• Similarity: 259/469 - (55%)
• Gaps: 50/469 - (10%)

  Fly    21 TANPD-AKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYD 84
            |||.. |::|:.||..:|:..:|||.:....|.|.:::.|||:.|::.:|||:|..||:.|:|:|
  Rat    25 TANGKYAQKLFSDLFEDYSSALRPVEDTDAVLNVTLQVTLSQIKDMDERNQILTAYLWIRQTWHD 89

  Fly    85 YKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSS 149
            ..|.|:..:|..::.:.:|||.:|||||||||.||......:.|...|.|.|.:.|..|||.|||
  Rat    90 AYLTWDRDQYDRLDSIRIPSDLVWRPDIVLYNKADDESSEPVNTNVVLRYDGLITWDSPAITKSS 154

  Fly   150 CEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPA 214
            |.:||.|||||.|.|.:.||||||:|.|||:        .|.::.| |||:|...|||::..:||
  Rat   155 CVVDVTYFPFDSQQCNLTFGSWTYNGNQVDI--------FNALDSG-DLSDFIEDVEWEVHGMPA 210

  Fly   215 VRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISI 279
            |:|...|.||.|||.|:||.:.::|::.||.|||:|||:.||||..|.||||:.|||||||.::|
  Rat   211 VKNVISYGCCSEPYPDVTFTLLLKRRSSFYIVNLLIPCVLISFLAPLSFYLPAASGEKVSLGVTI 275

  Fly   280 LLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRT 344
            ||::|||.|::|||: |.|..|||:||:.:.||.|.|.|..:|::|:||||...:...:..|.:.
  Rat   276 LLAMTVFQLMVAEIM-PASENVPLIGKYYIATMALITASTALTIMVMNIHFCGAEARPVPHWAKV 339

  Fly   345 VFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRS 409
            |.:..:.|.|.    :|.:.|...|.|            ..|.|.....| .|::..|...|||:
  Rat   340 VILKYMSRILF----VYDVGESCLSPR------------HSQEPEQVTKV-YSKLPESNLKTSRN 387

  Fly   410 TSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQ 474
            ..  |..:.:...|:.. .:|..||..                  |.|.:..:..:|...| .:.
  Rat   388 KD--LSRKKEVRKLLKN-DLGYQGGIP------------------QNTDSYCARYEALTKN-IEY 430

  Fly   475 TGGSLLDHPLTPTK 488
            ....|.||..|.:|
  Rat   431 IAKCLKDHKATNSK 444

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	94/218 (43%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	51/88 (58%)
		TM1 helix	242..266	CDD:349866	15/23 (65%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	8/21 (38%)
		Neur_chan_memb	<683..760	CDD:460753
Chrna9	NP_075219.2	LIC	8..474	CDD:455710	179/469 (38%)
		LGIC_ECD_nAChR_A9	31..237	CDD:349823	94/214 (44%)
		Cys-loop	155..169	CDD:349823	9/13 (69%)