Protein Alignment nAChRalpha3 and chrna9a

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_021326868.1
• Gene: chrna9a / 568807
• ZFINID: ZDB-GENE-090312-63
• Length: 580
• Species: Danio rerio
• Alignment Length: 780
• Identity: 221/780 - (28%)
• Similarity: 335/780 - (42%)
• Gaps: 217/780 - (27%)

  Fly     1 MKWFQVTIDMILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDV 65
            ||...:.|.:.|:|..:.|:... .|::|.:||:..|:..:|||.:...||.|.:::.|||:.|:
Zfish     1 MKTIFLVIHISLLLHAVFSAQGR-FAQKLLNDLMEGYSNALRPVEDTDKALNVTLQITLSQIKDM 64

  Fly    66 NLKNQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLA--- 127
            :.:||::||.|||.|.|:|..|.|:.:||.|:|::.:||:.:||||||||||||   |...:
Zfish    65 DERNQVLTTYLWVRQIWHDAYLSWDKEEYDGLEVIRIPSNLVWRPDIVLYNNAD---EEDSSGPP 126

  Fly   128 -TKATLNYTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNV 191
             |...|.|.|.:.|..|||.||||::||.|||||.|.|.:.||||||:|.|||
Zfish   127 DTNVVLRYNGEITWDSPAITKSSCKVDVSYFPFDSQECNLTFGSWTYNGNQVD------------ 179

  Fly   192 VEVGV---DLSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCM 253
            :.:|:   |||:|..:|||:...:|||:|...|.||.:||.|||:.:.::|::.||..||::||.
Zfish   180 IAMGMESGDLSDFVDNVEWECHGMPAVKNVIMYGCCSDPYPDITYTVLLKRRSSFYIFNLLLPCF 244

  Fly   254 GISFLTILVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFS 318
            .||||..|.||||:||||||||.:::||:||||.|::||.:|| |..|||:||:.:.||.:.|.|
Zfish   245 LISFLAPLGFYLPADSGEKVSLGVTVLLALTVFQLMVAESMPP-SESVPLIGKYYIATMTMITAS 308

  Fly   319 ICVTVLVLNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLEL 383
            ..:|:.::||||...:...:..|.:.:.|:.:.:...    :|.:.|           .|...|
Zfish   309 TSLTIFIMNIHFCGAEAKPVPHWAKVLIIDYMSKIFF----VYEVGE-----------NCTTPE- 357

  Fly   384 RDQIP--PLPPPVALSRMHHSPKTTSRSTSPHLQHRVQT-YNLMDECGMGMGGGASTITGHLSSL 445
            .|:.|  ...|..:|.|..:..|......  ||..|::: :|.........|.|..    |.:|.
Zfish   358 SDRGPFFSEDPLASLERDGYFDKGFYEDC--HLDERIRSQFNGYSHRDHRHGNGYH----HKNSS 416

  Fly   446 YPPTMSTANQQT--STTSSLIDAQYHNQYQ----QTGGSLLDHPLTPTKFRQMTSTSSQTGQNGS 504
            |........::|  |:.|.:..:.:|.:|.    :.|...|  |||..:..:.:..|.....||.
Zfish   417 YRQHRHEQQRRTRSSSNSPVRQSSHHPKYTHFIGRDGSEKL--PLTSQEKLKDSEISIPEKINGY 479

  Fly   505 GNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVN 569
            ....|||...|.|       |...:..|..|..      .::..|||
Zfish   480 TYDQGNGYLNGVG-------YLHDNGYSKTFGA------DSYNKTTT------------------ 513

  Fly   570 SVATASTLADSCCSGTIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTT 634
                                   |.|..|
Zfish   514 -----------------------GTGCVC------------------------------------ 519

  Fly   635 SAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQ 699
                                                                |.|
Zfish   520 ----------------------------------------------------GKH---------- 522

  Fly   700 RWHTCPELHKAMDGVTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQA 764
                    .|.:..:.|||:..|::........:||.||.|:||.|:|:|.|.|.:.:..::.:|
Zfish   523 --------QKLVRNIEYIANCFREQRGHQAKGAEWKKVAKVMDRFFMWVFFIMVFLMSILVLAKA 579

  Fly   765  764
            Zfish   580  579

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	98/224 (44%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	48/88 (55%)
		TM1 helix	242..266	CDD:349866	13/23 (57%)
		TM2 helix	274..295	CDD:349866	11/20 (55%)
		TM3 helix	306..328	CDD:349866	6/21 (29%)
		Neur_chan_memb	<683..760	CDD:460753	19/76 (25%)
chrna9a	XP_021326868.1	LGIC_ECD_nAChR_A9	25..232	CDD:349823	98/221 (44%)
		Cys-loop	150..164	CDD:349823	9/13 (69%)
		LGIC_TM_cation	233..>320	CDD:349853	47/87 (54%)
		TM1 helix	233..257	CDD:349853	13/23 (57%)
		TM2 helix	265..286	CDD:349853	11/20 (55%)
		TM3 helix	296..318	CDD:349853	6/21 (29%)