Protein Alignment nAChRalpha3 and chrnb4

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_696993.2
• Gene: chrnb4 / 568566
• ZFINID: ZDB-GENE-120809-1
• Length: 525
• Species: Danio rerio
• Alignment Length: 759
• Identity: 243/759 - (32%)
• Similarity: 333/759 - (43%)
• Gaps: 282/759 - (37%)

  Fly    10 MILVLSFISSSTANPDAKRLYDDLLS--NYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIM 72
            ::..||...|.......:||.:.||.  .||||:||.:|.|:.:||.:::.|:|||.||.:.|||
Zfish    21 LLCALSLARSGVCADSEERLMNWLLGKHRYNKLIRPALNRTERVTVLLQVSLAQLISVNEREQIM 85

  Fly    73 TTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGR 137
            |||:|:.|:|.||:|.|:|.||.|::.|.:||.|||.|||||||||||.:|||:.|.|.:.:.|.
Zfish    86 TTNVWLTQNWNDYRLSWDPAEYEGIDKLRIPSRHIWLPDIVLYNNADGTYEVTVFTNAIVLFNGS 150

  Fly   138 VEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFY 202
            :.|.|||||||:|:|:|::||||:|.|.:||.|||||..::||.         :......:.:|.
Zfish   151 IAWLPPAIYKSACKIEVKHFPFDQQNCTLKFRSWTYDHTEIDLL---------LKSDAASMDDFT 206

  Fly   203 TSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPS 267
            .|.|||||.:|..|.   .:..|..|:|:|::..::||.||||:||||||:.|:.|.||||||||
Zfish   207 PSGEWDILALPGRRT---VSPLDATYVDLTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPS 268

  Fly   268 DSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRS 332
            |.|||::|.||:||:||||.||:::|:|||||.|||:||:::|||:|.||||..:|.|||:|.||
Zfish   269 DCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIITSVCVLNVHHRS 333

  Fly   333 PQTHTMAPWVRTVFINQLPRFLVMRRPL-YPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVAL 396
            |.||:|..||:.||:.:||..|.|:||. :...:.::.:|||       |.|.|
Zfish   334 PSTHSMPAWVKIVFLVRLPALLFMQRPRGHSARQRLRQNRRL-------LGLAD----------- 380

  Fly   397 SRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTS 461
                    |..||.                                             ..|:.|
Zfish   381 --------TPGRSV---------------------------------------------MCSSAS 392

  Fly   462 SLIDAQYHNQYQQTGGSLLDHP-LTPTKFRQMTSTSSQTGQNGSGNGNG-NGGYGGHGQSGGHHL 524
            :|:.|        :..|.|..| |...|           |..||....| |.|:|......|:.|
Zfish   393 ALLAA--------SSASALSSPGLLYNK-----------GHFGSRRAEGLNNGHGSAQDLKGNRL 438

  Fly   525 YRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIH 589
            |
Zfish   439 Y---------------------------------------------------------------- 439

  Fly   590 QGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAA 654
                                                                             
Zfish   440 ----------------------------------------------------------------- 439

  Fly   655 AAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIAD 719
                                       |:                 |.:  |:.:|:|||.|:||
Zfish   440 ---------------------------CE-----------------WGS--EIQEAVDGVRYVAD 458

  Fly   720 QTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763
            .....::...|.|||||||||:||||||||.|..||||.|:.||
Zfish   459 HMMGNDDDQSVIEDWKYVAMVVDRLFLWIFVIVCVVGTLGLFLQ 502

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	101/219 (46%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	59/88 (67%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	32/76 (42%)
chrnb4	XP_696993.2	LIC	14..501	CDD:455710	241/756 (32%)
		LGIC_ECD_nAChR_B4	64..241	CDD:349828	87/188 (46%)
		Cys-loop	163..177	CDD:349828	8/13 (62%)