Protein Alignment nAChRalpha3 and chrna3

DIOPT Version :9

• Sequence 1: NP_001285015.1
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_001921314.1
• Gene: chrna3 / 568467
• ZFINID: ZDB-GENE-070822-1
• Length: 508
• Species: Danio rerio
• Alignment Length: 756
• Identity: 253/756 - (33%)
• Similarity: 353/756 - (46%)
• Gaps: 277/756 - (36%)

  Fly    14 LSFISSSTANPDAK-RLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLW 77
            |.|:.|.....:|: ||:..:.|:||:.:|||.||:|.:.|:.::.:|||:.|:..||||.||||
Zfish    16 LCFLLSGVCCSEAEHRLFSVIFSSYNQYIRPVENVSDPVVVQFEVSMSQLVKVDEVNQIMETNLW 80

  Fly    78 VEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRP 142
            :...|.||||:|:||::||||.:.|||:.||:|||||||||.|:|:|...|||.|.:.|.|.|.|
Zfish    81 LRHIWNDYKLRWDPKDFGGVEFIRVPSNKIWKPDIVLYNNAVGDFQVDDKTKALLRFNGDVTWIP 145

  Fly   143 PAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVG--VDLSEFYTSV 205
            |||:||||:|||.|||||.|.|.|||||||||..::||           |.:|  ::|.:|:.|.
Zfish   146 PAIFKSSCKIDVTYFPFDYQNCTMKFGSWTYDKAKIDL-----------VLIGSTINLKDFWESG 199

  Fly   206 EWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSG 270
            ||.|::.|..:::..|.||:|.|.|||:::.:||..||||:|:||||:.|||||:||||||||.|
Zfish   200 EWTIIDAPGYKHDIKYNCCEEIYTDITYSLYIRRLPLFYTINMIIPCLLISFLTVLVFYLPSDCG 264

  Fly   271 EKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQT 335
            |||:|.||:|||||||.|::.|.||.||||:||:|:::|||||..|.||.:||.|||:|:|:|:|
Zfish   265 EKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPKT 329

  Fly   336 HTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMH 400
            |||..|||.||::.|||.:.|.||                      |...::|
Zfish   330 HTMPCWVRRVFLSLLPRVMFMTRP----------------------EKDQEVP------------ 360

  Fly   401 HSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLID 465
                                   ..:|                |::||..|   :|||..
Zfish   361 -----------------------AKQC----------------SVHPPVSS---KQTSVC----- 378

  Fly   466 AQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSS 530
                     .|..||   |.||:..:.:.|:....:                    ||.:::
Zfish   379 ---------RGPQLL---LCPTELNEASRTAFLCRE--------------------HHCWKK--- 408

  Fly   531 CSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGA- 594
                           |.|..||:                                       ||
Zfish   409 ---------------HISNMTSE---------------------------------------GAE 419

  Fly   595 --GAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAA 657
              |:.|.|||.|
Zfish   420 DGGSPCSSSESL----------------------------------------------------- 431

  Fly   658 AAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTR 722
                                        .|..|.|.         ..|::.:|::.|.|||:..|
Zfish   432 ----------------------------DGFLCMSA---------VSPQVREAIESVKYIAENMR 459

  Fly   723 KEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763
            .:.|:..|::||||||||:||:|||:|.:..::||||:.||
Zfish   460 LQNEAKEVQDDWKYVAMVIDRIFLWVFVLVCILGTAGLFLQ 500

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_001285015.1	Neur_chan_LBD	26..241	CDD:280998	109/217 (50%)
		Neur_chan_memb	248..>359	CDD:280999	71/110 (65%)
		Neur_chan_memb	<683..760	CDD:280999	29/76 (38%)
chrna3	XP_001921314.1	LIC	4..499	CDD:273305	251/753 (33%)
		Neur_chan_LBD	29..235	CDD:280998	108/216 (50%)
		Neur_chan_memb	242..498	CDD:280999	134/515 (26%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	250	1.000	Domainoid score	I2065
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	473	1.000	Inparanoid score	I1461
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	1	1.000	-	-		FOG0000583
OrthoInspector	1	1.000	-	-		mtm6399
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	1	1.100	-	-	O	PTHR18945
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.970