Protein Alignment nAChRalpha3 and chrna4b

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001041528.1
• Gene: chrna4b / 556619
• ZFINID: ZDB-GENE-090505-3
• Length: 627
• Species: Danio rerio
• Alignment Length: 787
• Identity: 285/787 - (36%)
• Similarity: 387/787 - (49%)
• Gaps: 218/787 - (27%)

  Fly    12 LVLSFI-SSSTANPDA-KRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTT 74
            |.|::: |.|.|:..| :||...|..|||||.|||.|::|.:.|...|.::|||||:.|||:|||
Zfish    15 LQLAYVFSMSPAHAHAEERLLQSLFINYNKLSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTT 79

  Fly    75 NLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVE 139
            |:||:|.|.||||:|.|:||..|..:.:||:.||||||||||||||:|.||..|||.:.|.||::
Zfish    80 NVWVKQEWNDYKLRWNPEEYENVTSIRIPSEIIWRPDIVLYNNADGDFAVTHLTKAQVFYNGRIK 144

  Fly   140 WRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTS 204
            |:|||||||||.|||.:||||:|.|.|||||||||..::||..:..         .||..:::.|
Zfish   145 WKPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDRAKIDLISMAS---------DVDQMDYWES 200

  Fly   205 VEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDS 269
            .||.|:......|.|.|.||.|.|.|||::..:||..||||:||||||:.||.||:|||||||:.
Zfish   201 GEWVIINAVGTYNIKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSEC 265

  Fly   270 GEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQ 334
            .||::|.||:|||||||.||:.||||.||||:||:|:::|||||..|.||.:||.|||:|.||.:
Zfish   266 AEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSSR 330

  Fly   335 THTMAPWVRTVFINQLPRFLVMRRPLYPIS---------EMI---KSSRRLMVRTCNGLELRDQ- 386
            ||:|..|||.:|::.:||:|.|:||  |.|         ||:   .:|:.:.:|. |.|::..|
Zfish   331 THSMPHWVRQLFLHLVPRYLFMKRP--PASGKRNCGKLIEMMHKQANSQSMFLRN-NILDISPQS 392

  Fly   387 -IPPLPPPVA-----LSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSL 445
             ||.|.|..|     :.:...|||.....:||..|     |:::.|
Zfish   393 PIPHLGPTSAAQQDQVQQDSTSPKPLFFCSSPSSQ-----YSILQE------------------- 433

  Fly   446 YPPTMSTANQQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGN 510
                   .|.||                         |.||.    ::.|.:.|
Zfish   434 -------ENSQT-------------------------PQTPV----LSYTCAYT----------- 451

  Fly   511 GGYGGHGQSGGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTT--TVNSVAT 573
                                            :...||:|||     .|:.:..|.  |:.....
Zfish   452 --------------------------------NSIFASSTTS-----FPDTLLGTLLHTIPQDGA 479

  Fly   574 ASTLADSCCSGTIQIHQG-----------MGAGAA--CQSSELLHHQQLHHQQMHHQQQQQQQQQ 625
            .||..|      .|.|..           :|||:.  |.:||....|:|         ...:..:
Zfish   480 KSTECD------FQGHSAESVFMEAKESVLGAGSCQHCLTSEGTSLQKL---------DLDEAGR 529

  Fly   626 HHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHC 690
            ...|..|         :|....||:..:....||...:
Zfish   530 CTRHNMP---------SAQIFSPSSDGSTDLNAAEPVS--------------------------- 558

  Fly   691 CSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVV 755
                 |:.|      .|.||::||.||||..|.|:....|:|||||||||:||:|||:|.:..::
Zfish   559 -----NLSQ------SLLKALEGVQYIADHLRAEDSDFSVREDWKYVAMVIDRIFLWMFVLVCIL 612

  Fly   756 GTAGIIL 762
            ||.|:.|
Zfish   613 GTVGLFL 619

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	114/218 (52%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	60/88 (68%)
		TM1 helix	242..266	CDD:349866	18/23 (78%)
		TM2 helix	274..295	CDD:349866	14/20 (70%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	31/76 (41%)
chrna4b	NP_001041528.1	LGIC_ECD	31..237	CDD:475126	113/214 (53%)
		Neur_chan_memb	244..618	CDD:460753	160/546 (29%)