Protein Alignment nAChRalpha3 and chrna10a

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: XP_021337092.1
• Gene: chrna10a / 556507
• ZFINID: ZDB-GENE-060503-725
• Length: 532
• Species: Danio rerio
• Alignment Length: 747
• Identity: 201/747 - (26%)
• Similarity: 298/747 - (39%)
• Gaps: 259/747 - (34%)

  Fly    26 AKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWE 90
            |::|.:||.:||...:|||.:..|.|.|.:::.|||:||::.:|||:|..||:.|.|.|..|.|.
Zfish    30 AQKLLNDLFNNYTSALRPVEDTEDILNVTLQITLSQIIDMDERNQILTAYLWIRQVWIDKYLTWN 94

  Fly    91 PKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVE 155
            .:.|.|::.:.:|..::|||||||||:||.:|..|:.|...:.:.|::.|..|||.||||::||.
Zfish    95 KENYDGLDTIRIPGSYVWRPDIVLYNSADDHFTSTMDTNVVIRHDGQIMWDAPAITKSSCKVDVS 159

  Fly   156 YFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRNEKF 220
            :||||.|.|.:.:|||||:|.|:|:        .|.:| ..||::...:|||::|.:||.||...
Zfish   160 FFPFDAQQCRLTYGSWTYNGNQLDI--------LNAME-SADLADLVDNVEWEVLGMPAKRNIIL 215

  Fly   221 YTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLSLTV 285
            |.||.:||.|:|:.:.::|:..||..||:|||:.||||..|.||||:||||||||.::::|:|||
Zfish   216 YGCCADPYPDVTYTLKLKRRASFYVFNLLIPCVMISFLAPLGFYLPADSGEKVSLGVTVMLALTV 280

  Fly   286 FFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFINQL 350
            |.||:|||:|| |..|||:||:.:.||.:.|.|..:|:.|:|||...|....:..|.:...:..|
Zfish   281 FQLLVAEIMPP-SENVPLIGKYYIATMTMITASTALTIFVMNIHHCGPDAKPVPDWAKKFILQYL 344

  Fly   351 PRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRSTSPHLQ 415
            .|...    :|.:.|...:.:                |..|.|              :...|
Zfish   345 ARICF----VYEVGENCMTPQ----------------PEKPDP--------------QPQKP--- 372

  Fly   416 HRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQTGGSLL 480
                     .:|...|.|.|    |....::   ....||:.::...|.|.:..:|......||.
Zfish   373 ---------QDCNCNMNGQA----GKDDFVF---KFDRNQEATSPKLLEDREDMSQILSPACSLG 421

  Fly   481 DHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQHAHQA 545
            .||
Zfish   422 KHP-------------------------------------------------------------- 424

  Fly   546 HASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQSSELLHHQQLH 610
                 |...|:....|..|.              .|..|
Zfish   425 -----TCRYGVWKEGVFMSM--------------DCGDG-------------------------- 444

  Fly   611 HQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPPAGV 675
                                                             ||..|.|
Zfish   445 -------------------------------------------------AAETGKP--------- 451

  Fly   676 CDILPACQREGGPHCCSGRGNVRQRWH--------TCPELHKAMDGVTYIADQTRKEEESTRVKE 732
                            .|||....|.|        .|.:.| .:..:.|:|:..|.:..:.|...
Zfish   452 ----------------IGRGQEGCRDHDSGFKSQCKCDQQH-LLRNIEYVANCYRDQRATARRTG 499

  Fly   733 DWKYVAMVLDRLFLWIFTIAV------VVGTA 758
            :||.||.||||.|:|:|.|.|      ::|.|
Zfish   500 EWKKVAKVLDRFFMWLFFIMVFFMSLLIMGKA 531

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	90/216 (42%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	51/88 (58%)
		TM1 helix	242..266	CDD:349866	14/23 (61%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	7/21 (33%)
		Neur_chan_memb	<683..760	CDD:460753	26/90 (29%)
chrna10a	XP_021337092.1	LIC	31..422	CDD:455710	162/453 (36%)
		LGIC_ECD_nAChR_A10	55..235	CDD:349824	79/188 (42%)
		Cys-loop	154..168	CDD:349824	8/13 (62%)