Protein Alignment nAChRalpha3 and chrna6

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001036149.1
• Gene: chrna6 / 555747
• ZFINID: ZDB-GENE-090312-91
• Length: 512
• Species: Danio rerio
• Alignment Length: 761
• Identity: 250/761 - (32%)
• Similarity: 340/761 - (44%)
• Gaps: 263/761 - (34%)

  Fly     5 QVTIDMILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKN 69
            :||:..:|:........::....||:..|...||:.:|||.||:|.:||..::.:|||:.|:..|
Zfish     6 RVTLFFLLITLITQDCFSSKGEDRLFRRLFRRYNQFIRPVENVSDPVTVEFEVSISQLVKVDEVN 70

  Fly    70 QIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNY 134
            |||.||||:...|.||||||.|.|:.|:|.:.|||:.||||||||||||.|:|.|...|||.|.:
Zfish    71 QIMETNLWLRHIWNDYKLKWSPAEFDGIEFIRVPSNKIWRPDIVLYNNAVGDFLVEDKTKALLKF 135

  Fly   135 TGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVG--VD 197
            .|.:.|.||||:||||.:|:.|||||.|.|.|||||||||..::||           |.:|  |:
Zfish   136 DGTITWVPPAIFKSSCPMDITYFPFDYQNCSMKFGSWTYDKAKIDL-----------VLIGSKVN 189

  Fly   198 LSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILV 262
            |.:|:.|.||:|::.|..:::..|.||:|.|.|||::..:||..||||:||||||:.|||||:||
Zfish   190 LKDFWESGEWEIIDAPGYKHDIKYNCCEEIYPDITYSFYIRRLPLFYTINLIIPCLLISFLTVLV 254

  Fly   263 FYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLN 327
            ||||||.||||:|.||:|||||||.|::.|.||.||||:||:|:::|||||..|.||.:||.|||
Zfish   255 FYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLN 319

  Fly   328 IHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPP 392
            :|:|:|.||||..||.|||:..|||.::|||||    ::.:||
Zfish   320 VHYRTPMTHTMPSWVHTVFLRALPRIMLMRRPL----DLSESS---------------------- 358

  Fly   393 PVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQT 457
                                                 |.||
Zfish   359 -------------------------------------GKGG------------------------ 362

  Fly   458 STTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGH 522
                           .:||||                         ||.|.|.||.|...::.|
Zfish   363 ---------------LETGGS-------------------------SGTGAGRGGEGKKRKNSG- 386

  Fly   523 HLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQ 587
               .||.:                                     :||:......|
Zfish   387 ---SQQGA-------------------------------------MNSLEFGEGKA--------- 402

  Fly   588 IHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSA 652
            ..:|...|..|                                .|...|........:...|..|
Zfish   403 ALEGKKGGCPC--------------------------------HPLKEATEGDCGKVSRQLSPQA 435

  Fly   653 AAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYI 717
            .....|.:..:                                         ||:.:|::.|.||
Zfish   436 INTVVAFSVVS-----------------------------------------PEIKQAIESVKYI 459

  Fly   718 ADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763
            |:..|...::..|::||||||||:||:|||:|.:..|:||.|:.||
Zfish   460 AENMRSRNKAKEVEDDWKYVAMVIDRIFLWVFVLVCVLGTLGLFLQ 505

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	107/219 (49%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	62/88 (70%)
		TM1 helix	242..266	CDD:349866	19/23 (83%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	26/76 (34%)
chrna6	NP_001036149.1	LIC	38..504	CDD:455710	242/726 (33%)
		LGIC_ECD	38..233	CDD:475126	104/205 (51%)