Protein Alignment nAChRalpha3 and chrna5

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001017885.1
• Gene: chrna5 / 550584
• ZFINID: ZDB-GENE-050417-440
• Length: 466
• Species: Danio rerio
• Alignment Length: 785
• Identity: 222/785 - (28%)
• Similarity: 306/785 - (38%)
• Gaps: 345/785 - (43%)

  Fly    12 LVLSFISSSTANPD-----AK---RLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLK 68
            ||.|.:..|...|.     ||   :|:..|.|||.|.|||:.::...:.|:..|.:|||:||:.|
Zfish    14 LVCSAVLCSAIGPPMLSSYAKTEDKLFKHLFSNYQKWVRPIEDLNTTIQVKFGLAISQLVDVDEK 78

  Fly    69 NQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLN 133
            ||:||||:|::|.|.|.||:|:|..|.|:..:.||||.||.||||||:|||||||.|: |||.:.
Zfish    79 NQLMTTNVWMKQEWTDLKLRWDPDHYLGITSIRVPSDSIWIPDIVLYDNADGNFEATV-TKAVVR 142

  Fly   134 YTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDL 198
            :.|.:.|.|||.|||:|.|||.:||||.|.|.||||||||||.|||:.         :.:|.||.
Zfish   143 FDGTISWTPPANYKSACTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIL---------LEDVHVDK 198

  Fly   199 SEFYTSVEWDILEVPAVRNEK----FYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLT 259
            .:::.:.||:|::....|..:    |:      |..||::..:||..||||:.|||||:|:||||
Zfish   199 RDYFDNGEWEIVKATGNRGLRRDGTFF------YPSITYSFIIRRLPLFYTLFLIIPCIGLSFLT 257

  Fly   260 ILVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVL 324
            :|||||||:.|||:||..|:|:|||||.|::.||||.:|.|:||:|::::||||..|.||.:||.
Zfish   258 VLVFYLPSNGGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVITVF 322

  Fly   325 VLNIHFRSPQT-HTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIP 388
            .:|||.||..| |:||||||.:|::.|||.|.||
Zfish   323 AINIHHRSSSTHHSMAPWVRRIFLHHLPRLLCMR------------------------------- 356

  Fly   389 PLPPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTA 453
                                                                             
Zfish   357 ----------------------------------------------------------------- 356

  Fly   454 NQQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQ 518
                    |.:|                        |..||..::||
Zfish   357 --------SHVD------------------------RYATSAGTRTG------------------ 371

  Fly   519 SGGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCS 583
                                                                             
Zfish   372 ----------------------------------------------------------------- 371

  Fly   584 GTIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGP 648
                   |:|.|.:..                 |...|...:|:
Zfish   372 -------GLGYGKSLS-----------------QDTNQLDAEHN--------------------- 391

  Fly   649 STSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDG 713
                                                                      |..|::.
Zfish   392 ----------------------------------------------------------LQAALES 398

  Fly   714 VTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMPIDIKL 778
            :.||.....||.|...|.:|||:||.||||:|||.|.:..::|:|  :|..|.:|.....|....
Zfish   399 IRYITRHVVKENEVREVVQDWKFVAQVLDRVFLWAFLLVSLLGSA--LLFIPVIYKWASIIVPSY 461

  Fly   779 SEIAS 783
            :||.|
Zfish   462 AEITS 466

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	102/229 (45%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	55/88 (63%)
		TM1 helix	242..266	CDD:349866	18/23 (78%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	9/21 (43%)
		Neur_chan_memb	<683..760	CDD:460753	23/76 (30%)
chrna5	NP_001017885.1	LIC	12..448	CDD:455710	216/765 (28%)
		LGIC_ECD_nAChR_A5	34..240	CDD:349819	101/221 (46%)
		Cys-loop	159..173	CDD:349819	9/13 (69%)