Protein Alignment nAChRalpha3 and chrna5

DIOPT Version :9

• Sequence 1: NP_001285015.1
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001017885.1
• Gene: chrna5 / 550584
• ZFINID: ZDB-GENE-050417-440
• Length: 466
• Species: Danio rerio
• Alignment Length: 785
• Identity: 222/785 - (28%)
• Similarity: 306/785 - (38%)
• Gaps: 345/785 - (43%)

  Fly    12 LVLSFISSSTANPD-----AK---RLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLK 68
            ||.|.:..|...|.     ||   :|:..|.|||.|.|||:.::...:.|:..|.:|||:||:.|
Zfish    14 LVCSAVLCSAIGPPMLSSYAKTEDKLFKHLFSNYQKWVRPIEDLNTTIQVKFGLAISQLVDVDEK 78

  Fly    69 NQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLN 133
            ||:||||:|::|.|.|.||:|:|..|.|:..:.||||.||.||||||:|||||||.|: |||.:.
Zfish    79 NQLMTTNVWMKQEWTDLKLRWDPDHYLGITSIRVPSDSIWIPDIVLYDNADGNFEATV-TKAVVR 142

  Fly   134 YTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDL 198
            :.|.:.|.|||.|||:|.|||.:||||.|.|.||||||||||.|||:.         :.:|.||.
Zfish   143 FDGTISWTPPANYKSACTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIL---------LEDVHVDK 198

  Fly   199 SEFYTSVEWDILEVPAVRNEK----FYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLT 259
            .:::.:.||:|::....|..:    |:      |..||::..:||..||||:.|||||:|:||||
Zfish   199 RDYFDNGEWEIVKATGNRGLRRDGTFF------YPSITYSFIIRRLPLFYTLFLIIPCIGLSFLT 257

  Fly   260 ILVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVL 324
            :|||||||:.|||:||..|:|:|||||.|::.||||.:|.|:||:|::::||||..|.||.:||.
Zfish   258 VLVFYLPSNGGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIVITVF 322

  Fly   325 VLNIHFRSPQT-HTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIP 388
            .:|||.||..| |:||||||.:|::.|||.|.||
Zfish   323 AINIHHRSSSTHHSMAPWVRRIFLHHLPRLLCMR------------------------------- 356

  Fly   389 PLPPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTA 453
                                                                             
Zfish   357 ----------------------------------------------------------------- 356

  Fly   454 NQQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQ 518
                    |.:|                        |..||..::||
Zfish   357 --------SHVD------------------------RYATSAGTRTG------------------ 371

  Fly   519 SGGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCS 583
                                                                             
Zfish   372 ----------------------------------------------------------------- 371

  Fly   584 GTIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGP 648
                   |:|.|.:..                 |...|...:|:
Zfish   372 -------GLGYGKSLS-----------------QDTNQLDAEHN--------------------- 391

  Fly   649 STSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDG 713
                                                                      |..|::.
Zfish   392 ----------------------------------------------------------LQAALES 398

  Fly   714 VTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMPIDIKL 778
            :.||.....||.|...|.:|||:||.||||:|||.|.:..::|:|  :|..|.:|.....|....
Zfish   399 IRYITRHVVKENEVREVVQDWKFVAQVLDRVFLWAFLLVSLLGSA--LLFIPVIYKWASIIVPSY 461

  Fly   779 SEIAS 783
            :||.|
Zfish   462 AEITS 466

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_001285015.1	Neur_chan_LBD	26..241	CDD:280998	102/221 (46%)
		Neur_chan_memb	248..>359	CDD:280999	68/111 (61%)
		Neur_chan_memb	<683..760	CDD:280999	23/76 (30%)
chrna5	NP_001017885.1	LIC	12..448	CDD:273305	216/765 (28%)
		Neur_chan_LBD	36..239	CDD:280998	100/218 (46%)
		Neur_chan_memb	246..446	CDD:280999	104/515 (20%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.820