Protein Alignment nAChRalpha3 and Chrnd

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_062171.1
• Gene: Chrnd / 54240
• RGDID: 2352
• Length: 517
• Species: Rattus norvegicus
• Alignment Length: 773
• Identity: 201/773 - (26%)
• Similarity: 301/773 - (38%)
• Gaps: 286/773 - (36%)

  Fly     7 TIDMILVLSFISSSTANPDAKRLYDDLL--SNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKN 69
            |:.::..|....|...| :.:||...|.  ..|||.:|||....|.:.|.:.|.||.||.:....
  Rat     7 TLGLLAALVVCGSWGLN-EEQRLIQHLFEEKGYNKELRPVARKEDIVDVALSLTLSNLISLKEVE 70

  Fly    70 QIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNY 134
            :.:|||:|::.:|.|.:|:|...|:|.:.:|.:|||.:|.|:|||.||.||:|:::.|....::.
  Rat    71 ETLTTNVWIDHAWIDSRLQWNANEFGNITVLRLPSDMVWLPEIVLENNNDGSFQISYACNVLVSD 135

  Fly   135 TGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDG--FQVDLRHIDELNGTNVVE-VGV 196
            :|.|.|.||||::|||.|.|.|||||.|.|.:||.|..|..  .::.|:..:|.|.:..:| :.:
  Rat   136 SGHVTWLPPAIFRSSCPISVTYFPFDWQNCSLKFSSLKYTAKEIRLSLKQEEEDNRSYPIEWIII 200

  Fly   197 DLSEFYTSVEWDILEVPAVRNEKFYTCCDEP-YLDITFNITMRRKTLFYTVNLIIPCMGISFLTI 260
            |...|..:.||:|:...|..|.......|.. :.|:||.:.:|||.|||.:|:::||:.|||:..
  Rat   201 DPEGFTENGEWEIVHRAAKVNVDPSVPMDSTNHQDVTFYLIIRRKPLFYIINILVPCVLISFMIN 265

  Fly   261 LVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLV 325
            ||||||.|.|||.|::||:||:.:||.||:::.:|.||:.:||:|||:||.|:|.|..:.:.|:|
  Rat   266 LVFYLPGDCGEKTSVAISVLLAQSVFLLLISKRLPATSMAIPLVGKFLLFGMVLVTMVVVICVIV 330

  Fly   326 LNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPL 390
            ||||||:|.||.::..|:..|:..||:.|.|.||                      |..|
  Rat   331 LNIHFRTPSTHVLSEGVKKFFLETLPKLLHMSRP----------------------EEED----- 368

  Fly   391 PPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQ 455
            |.|.||.|
  Rat   369 PGPRALIR--------------------------------------------------------- 376

  Fly   456 QTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSG 520
               .||||                                                ||
  Rat   377 ---RTSSL------------------------------------------------GY------- 383

  Fly   521 GHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGT 585
                   .|.....||                         :||
  Rat   384 -------ISKAEEYFS-------------------------LKS--------------------- 395

  Fly   586 IQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPST 650
                          .|:|:.              ::|.::|...:|.||:.
  Rat   396 --------------RSDLMF--------------EKQSERHGLARRLTTAR-------------- 418

  Fly   651 SAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVT 715
                               .|||                   ....|:|....  |:..|:||..
  Rat   419 -------------------KPPA-------------------SSEQVQQELFN--EMKPAVDGAN 443

  Fly   716 YIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMP 773
            :|.:..|.:......|::|..||..:|||.|::.|..:|||||.|.||.  :|:...|
  Rat   444 FIVNHMRDQNSYNEEKDNWNQVARTVDRLCLFVVTPVMVVGTAWIFLQG--VYNQPPP 499

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	82/223 (37%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	47/88 (53%)
		TM1 helix	242..266	CDD:349866	13/23 (57%)
		TM2 helix	274..295	CDD:349866	9/20 (45%)
		TM3 helix	306..328	CDD:349866	10/21 (48%)
		Neur_chan_memb	<683..760	CDD:460753	22/76 (29%)
Chrnd	NP_062171.1	LGIC_ECD	26..246	CDD:475126	81/219 (37%)
		Neur_chan_memb	253..489	CDD:460753	106/512 (21%)