Protein Alignment nAChRalpha3 and Chrnb2

DIOPT Version :9

• Sequence 1: NP_001285015.1
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_062170.1
• Gene: Chrnb2 / 54239
• RGDID: 2350
• Length: 500
• Species: Rattus norvegicus
• Alignment Length: 755
• Identity: 239/755 - (31%)
• Similarity: 330/755 - (43%)
• Gaps: 296/755 - (39%)

  Fly    14 LSFISSSTANPDA-KRLYDDLL--SNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTN 75
            |.::.|.....|. :||.:.||  |.||||:||..|.::.:||::.:.|:|||.|:.:.||||||
  Rat    15 LLWLCSGVLGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTN 79

  Fly    76 LWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEW 140
            :|:.|.|.||:|.|:|:::..::.:.:||.|||.||:||||||||.:||:..:.|.::|.|.:.|
  Rat    80 VWLTQEWEDYRLTWKPEDFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFW 144

  Fly   141 RPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGV-DLSEFYTS 204
            .|||||||:|:|:|::||||:|.|.|||.|||||..::||          |::..| .|.:|..|
  Rat   145 LPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDL----------VLKSDVASLDDFTPS 199

  Fly   205 VEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDS 269
            .||||:.:|..|||   ...|..|:|||::..:|||.||||:||||||:.|:.|.|||||||||.
  Rat   200 GEWDIIALPGRRNE---NPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDC 261

  Fly   270 GEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQ 334
            |||::|.||:||:||||.||:::|:|||||.|||:||:::|||:|.||||..:|.|||:|.|||.
  Rat   262 GEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPT 326

  Fly   335 THTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRM 399
            ||||||||:.||:.:||..|.:::|.:..:.     :||.:|
  Rat   327 THTMAPWVKVVFLEKLPTLLFLQQPRHRCAR-----QRLRLR----------------------- 363

  Fly   400 HHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLI 464
                                                                             
  Rat   364 ----------------------------------------------------------------- 363

  Fly   465 DAQYHNQYQQTG-GSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQ 528
                ..|.::.| |:|.        ||:
  Rat   364 ----RRQREREGAGALF--------FRE------------------------------------- 379

  Fly   529 SSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMG 593
                                         .|.....|..||..:.                ||:
  Rat   380 -----------------------------GPAADPCTCFVNPASV----------------QGL- 398

  Fly   594 AGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAA 658
                                                      |.|..|...|.||..|.
  Rat   399 ------------------------------------------AGAFRAEPTAAGPGRSV------ 415

  Fly   659 AAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRK 723
                                        ||..|.              |.:|:|||.:|||..|.
  Rat   416 ----------------------------GPCSCG--------------LREAVDGVRFIADHMRS 438

  Fly   724 EEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763
            |::...|:|||||||||:||||||||....|.||.|:.||
  Rat   439 EDDDQSVREDWKYVAMVIDRLFLWIFVFVCVFGTVGMFLQ 478

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_001285015.1	Neur_chan_LBD	26..241	CDD:280998	100/218 (46%)
		Neur_chan_memb	248..>359	CDD:280999	70/110 (64%)
		Neur_chan_memb	<683..760	CDD:280999	34/76 (45%)
Chrnb2	NP_062170.1	LIC	28..477	CDD:273305	234/739 (32%)
		Neur_chan_LBD	28..233	CDD:280998	100/217 (46%)
		Neur_chan_memb	240..476	CDD:280999	128/513 (25%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3645
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm9116
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.820