Protein Alignment nAChRalpha3 and nAChRbeta2

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_524483.1
• Gene: nAChRbeta2 / 42920
• FlyBaseID: FBgn0004118
• Length: 519
• Species: Drosophila melanogaster
• Alignment Length: 810
• Identity: 314/810 - (38%)
• Similarity: 398/810 - (49%)
• Gaps: 310/810 - (38%)

  Fly     3 WFQVTIDMILVLSFISSST-----ANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQL 62
            |....:.:.|::...:|:.     ||||.||||||||||||:|:|||||.|:.|||.:.||||||
  Fly     2 WHWSLLCVFLLVPLANSTAPISFEANPDTKRLYDDLLSNYNRLIRPVVNNTETLTVWLGLKLSQL 66

  Fly    63 IDVNLKNQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLA 127
            |:||||||:|||||||:|.|:||||:|:|:||||||.|:|||:|||.||||||||.|||:||||.
  Fly    67 IEVNLKNQVMTTNLWVKQRWFDYKLRWDPEEYGGVEQLYVPSEHIWVPDIVLYNNWDGNYEVTLM 131

  Fly   128 TKATLNYTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVV 192
            |||||.|||.|.|.||||||||||::|||||:|||.|.||||||||:|.||||:|:|::.|:|:|
  Fly   132 TKATLKYTGEVFWEPPAIYKSSCEMNVEYFPYDEQICFMKFGSWTYNGAQVDLKHLDQIPGSNLV 196

  Fly   193 EVGVDLSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISF 257
            :||:||:|||.|||||||||||.:||::|....||:.||||.:||||||||||||||:||:.::|
  Fly   197 QVGIDLTEFYLSVEWDILEVPATKNEEYYPDTLEPFSDITFKLTMRRKTLFYTVNLIVPCVALTF 261

  Fly   258 LTILVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVT 322
            ||:|||||||||||||:|.||||:||||||||||||||||||.||||||::||||||.:.|:..|
  Fly   262 LTVLVFYLPSDSGEKVTLCISILVSLTVFFLLLAEIIPPTSLAVPLLGKYLLFTMILVSLSVWTT 326

  Fly   323 VLVLNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNG----LEL 383
            |.|||||||||.||.|:|.||.:|::.:|:.::|||..|.:.:...|:      ..||    :::
  Fly   327 VCVLNIHFRSPSTHNMSPLVRKLFLHFMPKLMMMRRTQYTLPDYDDST------PSNGYTNEIDV 385

  Fly   384 RDQIPPLPPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPP 448
            ||.|...|
  Fly   386 RDSISDFP--------------------------------------------------------- 393

  Fly   449 TMSTANQQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGY 513
                        |...|:|                                          :|.|
  Fly   394 ------------SEFKDSQ------------------------------------------DGAY 404

  Fly   514 GGHGQSGGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMAN----PNVIKSTTTVNSVATA 574
                                                   |.||.|    .|||....|
  Fly   405 ---------------------------------------DNGMQNSVDSDNVIPRNLT------- 423

  Fly   575 STLADSCCSGTIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAA 639
                                                                             
  Fly   424 ----------------------------------------------------------------- 423

  Fly   640 AAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTC 704
                                                                             
  Fly   424 ----------------------------------------------------------------- 423

  Fly   705 PELHKAMDGVTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYD 769
            ||:.:|:..|.:||...:..::...:.||||:|:|||||.|||:||::.|.||..||.|:|:|||
  Fly   424 PEVLQALRAVRFIAQHIKDADKDNEIVEDWKFVSMVLDRFFLWLFTLSCVFGTLAIICQSPSLYD 488

  Fly   770 TRMPIDIKLSEIA----STTAKPNVARPVL 795
            ||.|||.:||||.    :....|::.|.||
  Fly   489 TRSPIDRQLSEIPLRKNNFMLPPDIVRQVL 518

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	159/217 (73%)
		Cys-loop	150..164	CDD:349832	10/13 (77%)
		LGIC_TM_nAChR	242..>331	CDD:349866	69/88 (78%)
		TM1 helix	242..266	CDD:349866	17/23 (74%)
		TM2 helix	274..295	CDD:349866	17/20 (85%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	24/76 (32%)
nAChRbeta2	NP_524483.1	LIC	9..479	CDD:455710	293/762 (38%)
		LGIC_ECD_nAChR_proto_alpha-like	29..247	CDD:349832	159/217 (73%)
		Cys-loop	154..168	CDD:349832	10/13 (77%)