Protein Alignment nAChRalpha3 and nAChRalpha4

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001097669.2
• Gene: nAChRalpha4 / 40521
• FlyBaseID: FBgn0266347
• Length: 539
• Species: Drosophila melanogaster
• Alignment Length: 770
• Identity: 378/770 - (49%)
• Similarity: 433/770 - (56%)
• Gaps: 245/770 - (31%)

  Fly    23 NPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKL 87
            ||||||||||||||||||||||||.||.|.|.|||||||||||||||||||||||||||||||||
  Fly     9 NPDAKRLYDDLLSNYNKLVRPVVNTTDVLKVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKL 73

  Fly    88 KWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEI 152
            :|||||||||.|||||||||||||||||||||||||||||||||:...|.|||:|||||||||||
  Fly    74 RWEPKEYGGVHMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYSEGLVEWKPPAIYKSSCEI 138

  Fly   153 DVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRN 217
            |||||||||||||:||||||||||:|||||:||..|:|||.|||||||||.||||||||||||||
  Fly   139 DVEYFPFDEQTCVLKFGSWTYDGFKVDLRHMDEQQGSNVVAVGVDLSEFYMSVEWDILEVPAVRN 203

  Fly   218 EKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLS 282
            ||||||||||||||||||||||||||||||:|||||||||||:|.|||||||||||:||||||:|
  Fly   204 EKFYTCCDEPYLDITFNITMRRKTLFYTVNIIIPCMGISFLTVLTFYLPSDSGEKVTLSISILIS 268

  Fly   283 LTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFI 347
            |.|||||:.|||||||||||||||:::|.|||.:.||||||:|||:||||||||.|||||:.|||
  Fly   269 LHVFFLLVVEIIPPTSLVVPLLGKYLIFAMILVSISICVTVVVLNVHFRSPQTHKMAPWVKRVFI 333

  Fly   348 NQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRSTSP 412
            :.||.||.::||.|    ..::|:.|:..|                       |:
  Fly   334 DFLPAFLFIKRPTY----NFETSKLLLKDT-----------------------HA---------- 361

  Fly   413 HLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQTGG 477
                          |                 .||...:|      ....|:.:.|:|...:..
  Fly   362 --------------C-----------------FYPYYSAT------NIDRLVSSGYNNSLPKED- 388

  Fly   478 SLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQHA 542
              |...:|                       .||.:||              ||..: .|:|...
  Fly   389 --LSQSIT-----------------------ANGPFGG--------------SCQVH-GPVPPLT 413

  Fly   543 HQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQSSELLHHQ 607
            |                                     |.|..|.....|..|.....|.:|::.
  Fly   414 H-------------------------------------CSSDEIAAVPDMDIGPLGMKSPILNNP 441

  Fly   608 QLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPP 672
            ...|.:
  Fly   442 AFSHSK----------------------------------------------------------- 447

  Fly   673 AGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKEDWKYV 737
                              |            .|::||:...|.:||:.|:.:|:||:||||||||
  Fly   448 ------------------C------------LPKIHKSCFCVRFIAEHTKMQEDSTKVKEDWKYV 482

  Fly   738 AMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMPI----DIKLSEIASTTAKP 788
            |||||||||||||:||||||||||||||||||.|:||    |:..|..::...:|
  Fly   483 AMVLDRLFLWIFTLAVVVGTAGIILQAPTLYDDRIPIIEQKDLDFSGTSAGRCRP 537

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	197/217 (91%)
		Cys-loop	150..164	CDD:349832	13/13 (100%)
		LGIC_TM_nAChR	242..>331	CDD:349866	71/88 (81%)
		TM1 helix	242..266	CDD:349866	20/23 (87%)
		TM2 helix	274..295	CDD:349866	15/20 (75%)
		TM3 helix	306..328	CDD:349866	13/21 (62%)
		Neur_chan_memb	<683..760	CDD:460753	39/76 (51%)
nAChRalpha4	NP_001097669.2	LIC	1..505	CDD:455710	361/736 (49%)
		LGIC_ECD_nAChR_proto_alpha-like	11..229	CDD:349832	197/217 (91%)
		Cys-loop	136..150	CDD:349832	13/13 (100%)