Protein Alignment nAChRalpha3 and nAChRalpha5

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001356885.1
• Gene: nAChRalpha5 / 34826
• FlyBaseID: FBgn0028875
• Length: 837
• Species: Drosophila melanogaster
• Alignment Length: 766
• Identity: 225/766 - (29%)
• Similarity: 315/766 - (41%)
• Gaps: 267/766 - (34%)

  Fly    11 ILVLSFISSST----ANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQI 71
            |.:|.:::.|.    |....|||..|||..||.|.|||:|.:|.|.:...|.|.|:|||:.|||:
  Fly   294 IFLLIYLNLSAKVCLAGYHEKRLLHDLLDPYNTLERPVLNESDPLQLSFGLTLMQIIDVDEKNQL 358

  Fly    72 MTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTG 136
            :.||:|::..|.|..|:|...:||||:.|.:|...||:||:::||:||..|:.|..|...:...|
  Fly   359 LVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNG 423

  Fly   137 RVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEF 201
            ...:.||.|:||:|:||:.:||||:|.|.||||||||||||:||:..||..|        |:|.:
  Fly   424 SCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGG--------DISSY 480

  Fly   202 YTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLP 266
            ..:.||::|.||..|||.:|.||.|||:||||.|.:||:||:|..||||||:.|:.:.:|.|.||
  Fly   481 VLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLP 545

  Fly   267 SDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFR 331
            .|||||:||.::||||||||..::||.:|.||..|||||.:....|.:...|:..|:|:||.|.|
  Fly   546 PDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHR 610

  Fly   332 SPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVAL 396
            :..||.|:.|:|.||:..||..|.|.||..|:.                ||.
  Fly   611 NADTHEMSEWIRIVFLCWLPWILRMSRPGRPLI----------------LEF------------- 646

  Fly   397 SRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTS 461
                  |.|....||...:|::                               :|....:..::.
  Fly   647 ------PTTPCSDTSSERKHQI-------------------------------LSDVELKERSSK 674

  Fly   462 SL------IDAQY-HNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQS 519
            ||      ||..: ||....|.|..|.|  .|..:|.:               .|.|..|..|..
  Fly   675 SLLANVLDIDDDFRHNCRPMTPGGTLPH--NPAFYRTV---------------YGQGDDGSIGPI 722

  Fly   520 GGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSG 584
            |                           ||...| .:.:...|||:|..
  Fly   723 G---------------------------STRMPD-AVTHHTCIKSSTEY---------------- 743

  Fly   585 TIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPS 649
                                                                             
  Fly   744 ----------------------------------------------------------------- 743

  Fly   650 TSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGV 714
                                                                    ||...:..:
  Fly   744 --------------------------------------------------------ELGLILKEI 752

  Fly   715 TYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAP 765
            .:|.||.||::|...:..|||:.|||:|||.|.|||:..::.|..::|.||
  Fly   753 RFITDQLRKDDECNDIANDWKFAAMVVDRLCLIIFTMFTILATIAVLLSAP 803

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	104/217 (48%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	45/88 (51%)
		TM1 helix	242..266	CDD:349866	11/23 (48%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	5/21 (24%)
		Neur_chan_memb	<683..760	CDD:460753	22/76 (29%)
nAChRalpha5	NP_001356885.1	LIC	313..802	CDD:455710	219/744 (29%)