Protein Alignment nAChRalpha3 and nAChRalpha7

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001162795.1
• Gene: nAChRalpha7 / 32928
• FlyBaseID: FBgn0086778
• Length: 564
• Species: Drosophila melanogaster
• Alignment Length: 764
• Identity: 229/764 - (29%)
• Similarity: 325/764 - (42%)
• Gaps: 267/764 - (34%)

  Fly    13 VLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLW 77
            :|..|.:..|.|..|||...||.|||.|.|||||.:|.|.:...|.|.|:|||:.|||::.||:|
  Fly    48 LLIMIPACAAGPHEKRLLHALLDNYNSLERPVVNESDPLQLSFGLTLMQIIDVDEKNQLLITNIW 112

  Fly    78 VEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRP 142
            ::..|.|..|:|...|:|||..|.:|...:|:||:::||:||..|:.|.||...:...|...:.|
  Fly   113 LKLEWNDMNLRWNSSEFGGVRDLRIPPHRLWKPDVLMYNSADEGFDGTYATNVVVRNNGSCLYVP 177

  Fly   143 PAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEW 207
            |.|:||:|:||:.:||||:|.|.||||||||||||:||:..||..|        |:|.|.|:.||
  Fly   178 PGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDEAGG--------DISSFITNGEW 234

  Fly   208 DILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEK 272
            |:|.||..|||.:|.||.|||:||||.|.:|||||:|..|||:||:.|:.:.:|.|.||.|||||
  Fly   235 DLLGVPGKRNEIYYNCCPEPYIDITFAILIRRKTLYYFFNLIVPCVLIASMALLGFTLPPDSGEK 299

  Fly   273 VSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHT 337
            :||.::||||||||..::||.:|.||..|||||.:....|.:...|:..|:|:||.|.|:|.||.
  Fly   300 LSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNPDTHE 364

  Fly   338 MAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHS 402
            |:.|:|.:|:..||..|.|:||    .::             |.|       .|||
  Fly   365 MSEWIRVIFLYWLPCILRMQRP----GQV-------------GYE-------CPPP--------- 396

  Fly   403 PKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSL---- 463
            |.::|.|.|...:.::|...|                               ::.|:.|.|
  Fly   397 PSSSSSSASGEKKQQIQNVEL-------------------------------KERSSKSLLANVL 430

  Fly   464 -IDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQ 527
             ||..:...::....:|   |..||.:|.|                                |||
  Fly   431 DIDDDFRCNHRCASATL---PHQPTYYRTM--------------------------------YRQ 460

  Fly   528 QSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGM 592
                                    .|.|...|           |..|..:.|.      ::|:.:
  Fly   461 ------------------------GDDGSVGP-----------VGPAGPVVDG------RLHEAI 484

  Fly   593 GAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAA 657
            .
  Fly   485 S---------------------------------------------------------------- 485

  Fly   658 AAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTC------PELHKAMDGVTY 716
                                                        |||      .||...:..:.:
  Fly   486 --------------------------------------------HTCLTSSAEYELALILKELRW 506

  Fly   717 IADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAP 765
            |.:|.:||:|::.:..|||:.|||:|||.|.|||:..::.|..::..||
  Fly   507 ITEQLKKEDETSDITRDWKFAAMVVDRLCLIIFTLFTIIATLAVLFSAP 555

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	109/217 (50%)
		Cys-loop	150..164	CDD:349832	8/13 (62%)
		LGIC_TM_nAChR	242..>331	CDD:349866	44/88 (50%)
		TM1 helix	242..266	CDD:349866	10/23 (43%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	5/21 (24%)
		Neur_chan_memb	<683..760	CDD:460753	24/82 (29%)
nAChRalpha7	NP_001162795.1	LIC	59..548	CDD:455710	223/744 (30%)