Protein Alignment nAChRalpha3 and Chrna4

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_077330.2
• Gene: Chrna4 / 25590
• RGDID: 2346
• Length: 630
• Species: Rattus norvegicus
• Alignment Length: 744
• Identity: 277/744 - (37%)
• Similarity: 373/744 - (50%)
• Gaps: 169/744 - (22%)

  Fly    27 KRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWEP 91
            :||...|.|.|||..|||.|::|.:.||..|.::|||||:.|||:||||:||:|.|:||||:|:|
  Rat    40 ERLLKRLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDP 104

  Fly    92 KEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEY 156
            .:|..|..:.:||:.||||||||||||||:|.||..|||.|.|.|||:|.|||||||||.|||.:
  Rat   105 GDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTF 169

  Fly   157 FPFDEQTCVMKFGSWTYDGFQVDL----RHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRN 217
            ||||:|.|.|||||||||..::||    ..:|:|             :|:.|.||.|::.....|
  Rat   170 FPFDQQNCTMKFGSWTYDKAKIDLVSMHSRVDQL-------------DFWESGEWVIVDAVGTYN 221

  Fly   218 EKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLS 282
            .:.|.||.|.|.|||:...:||..||||:||||||:.||.||:|||||||:.||||:|.||:|||
  Rat   222 TRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLCISVLLS 286

  Fly   283 LTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFI 347
            ||||.||:.||||.||||:||:|:::|||||..|.||.:||.|||:|.|||:||||..|||.||:
  Rat   287 LTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVFL 351

  Fly   348 NQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRSTSP 412
            :.:||.|.|:||     .::|.:.|.::.:.:.:....:.  .|.||.               .|
  Rat   352 DIVPRLLFMKRP-----SVVKDNCRRLIESMHKMANAPRF--WPEPVG---------------EP 394

  Fly   413 HLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQTGG 477
            .:        |.|.|..|: ..|.|...       ||.:....|.:..|..::.
  Rat   395 GI--------LSDICNQGL-SPAPTFCN-------PTDTAVETQPTCRSPPLEV----------- 432

  Fly   478 SLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQHA 542
                 |...|...:..|.....|                             ||     |.|:.:
  Rat   433 -----PDLKTSEVEKASPCPSPG-----------------------------SC-----PPPKSS 458

  Fly   543 HQAHASTTTSDLGMANPNVIKS-TTTVNSVATASTLADS---CCSGTIQIHQGMGAGAACQSSEL 603
            ..|             |.:||: :.:|..|.::...|:.   |.|.:||........|:...|
  Rat   459 SGA-------------PMLIKARSLSVQHVPSSQEAAEDGIRCRSRSIQYCVSQDGAASLADS-- 508

  Fly   604 LHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPP 668
                                       :||:|..:..|.     ||....:..|:........|.
  Rat   509 ---------------------------KPTSSPTSLKAR-----PSQLPVSDQASPCKCTCKEPS 541

  Fly   669 PPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKED 733
            |..|.   .:|.|...:..|          |.....|.|.:|::||.||||..:.|:....||||
  Rat   542 PVSPV---TVLKAGGTKAPP----------QHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKED 593

  Fly   734 WKYVAMVLDRLFLWIFTIAVVVGTAGIIL 762
            |||||||:||:|||:|.|..::||.|:.|
  Rat   594 WKYVAMVIDRIFLWMFIIVCLLGTVGLFL 622

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	114/219 (52%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	62/88 (70%)
		TM1 helix	242..266	CDD:349866	18/23 (78%)
		TM2 helix	274..295	CDD:349866	14/20 (70%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	32/76 (42%)
Chrna4	NP_077330.2	LGIC_ECD	39..245	CDD:475126	114/217 (53%)
		Neur_chan_memb	252..621	CDD:460753	157/516 (30%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	417..462		11/107 (10%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	504..525		7/54 (13%)