DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nAChRalpha3 and Chrna4

DIOPT Version :9

Sequence 1:	NP_001285015.1	Gene:	nAChRalpha3 / 31767	FlyBaseID:	FBgn0015519	Length:	795	Species:	Drosophila melanogaster
Sequence 2:	XP_038960311.1	Gene:	Chrna4 / 25590	RGDID:	2346	Length:	802	Species:	Rattus norvegicus

Alignment Length:	745	Identity:	276/745 - (37%)
Similarity:	374/745 - (50%)	Gaps:	171/745 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 KRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWEP 91
            :||...|.|.|||..|||.|::|.:.||..|.::|||||:.|||:||||:||:|.|:||||:|:|
  Rat   200 ERLLKRLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDP 264

  Fly    92 KEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEY 156
            .:|..|..:.:||:.||||||||||||||:|.||..|||.|.|.|||:|.|||||||||.|||.:
  Rat   265 GDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTF 329

  Fly   157 FPFDEQTCVMKFGSWTYDGFQVDL----RHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRN 217
            ||||:|.|.|||||||||..::||    ..:|:|             :|:.|.||.|::.....|
  Rat   330 FPFDQQNCTMKFGSWTYDKAKIDLVSMHSRVDQL-------------DFWESGEWVIVDAVGTYN 381

  Fly   218 EKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLS 282
            .:.|.||.|.|.|||:...:||..||||:||||||:.||.||:|||||||:.||||:|.||:|||
  Rat   382 TRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLCISVLLS 446

  Fly   283 LTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFI 347
            ||||.||:.||||.||||:||:|:::|||||..|.||.:||.|||:|.|||:||||..|||.||:
  Rat   447 LTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVFL 511

  Fly   348 NQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRST-S 411
            :.:||.|.|:||     .::|.:.|.::.                  ::.:|.::|:...... .
  Rat   512 DIVPRLLFMKRP-----SVVKDNCRRLIE------------------SMHKMANAPRFWPEPVGE 553

  Fly   412 PHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQTG 476
            |.:        |.|.|..|: ..|.|...       ||.:....|.:..|..::.
  Rat   554 PGI--------LSDICNQGL-SPAPTFCN-------PTDTAVETQPTCRSPPLEV---------- 592

  Fly   477 GSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQH 541
                  |...|...:..|.....|                             ||     |.|:.
  Rat   593 ------PDLKTSEVEKASPCPSPG-----------------------------SC-----PPPKS 617

  Fly   542 AHQAHASTTTSDLGMANPNVIKS-TTTVNSVATASTLADS---CCSGTIQIHQGMGAGAACQSSE 602
            :..|             |.:||: :.:|..|.::...|:.   |.|.:||........|:...|
  Rat   618 SSGA-------------PMLIKARSLSVQHVPSSQEAAEDGIRCRSRSIQYCVSQDGAASLADS- 668

  Fly   603 LLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPP 667
                                        :||:|..:..|.     ||....:..|:........|
  Rat   669 ----------------------------KPTSSPTSLKAR-----PSQLPVSDQASPCKCTCKEP 700

  Fly   668 PPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKE 732
            .|..|.   .:|.|...:..|          |.....|.|.:|::||.||||..:.|:....|||
  Rat   701 SPVSPV---TVLKAGGTKAPP----------QHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKE 752

  Fly   733 DWKYVAMVLDRLFLWIFTIAVVVGTAGIIL 762
            ||||||||:||:|||:|.|..::||.|:.|
  Rat   753 DWKYVAMVIDRIFLWMFIIVCLLGTVGLFL 782

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_001285015.1	Neur_chan_LBD	26..241	CDD:280998	114/217 (53%)
		Neur_chan_memb	248..>359	CDD:280999	73/110 (66%)
		Neur_chan_memb	<683..760	CDD:280999	32/76 (42%)
Chrna4	XP_038960311.1	LGIC_ECD	199..405	CDD:355788	114/217 (53%)
		Cys-loop	323..337	CDD:349787	9/13 (69%)
		Neur_chan_memb	412..781	CDD:397193	156/517 (30%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I1995
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	477	1.000	Inparanoid score	I1422
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D188416at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm12327
orthoMCL	1	0.900	-	-		OOG6_101158
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X294
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	9	8.870