Protein Alignment nAChRalpha3 and Chrna3

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_434692.2
• Gene: Chrna3 / 25101
• RGDID: 2345
• Length: 499
• Species: Rattus norvegicus
• Alignment Length: 756
• Identity: 247/756 - (32%)
• Similarity: 350/756 - (46%)
• Gaps: 279/756 - (36%)

  Fly    10 MILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTT 74
            ::|||..:.:::|:....||:..|..:||:::|||.||:..:.::.::.:|||:.|:..||||.|
  Rat    13 LMLVLMLLPAASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMET 77

  Fly    75 NLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVE 139
            |||::|.|.||||||:|.:|.|||.:.||::.||:|||||||||||:|:|...|||.|.|||.|.
  Rat    78 NLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVT 142

  Fly   140 WRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVG--VDLSEFY 202
            |.||||:||||:|||.|||||.|.|.||||||:||..::||           |.:|  ::|.:::
  Rat   143 WIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDL-----------VLIGSSMNLKDYW 196

  Fly   203 TSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPS 267
            .|.||.|::.|..::|..|.||:|.|.|||:::.:||..||||:||||||:.|||||:|||||||
  Rat   197 ESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPS 261

  Fly   268 DSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRS 332
            |.||||:|.||:|||||||.|::.|.||.||||:||:|:::|||||..|.||.:||.|||:|:|:
  Rat   262 DCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRT 326

  Fly   333 PQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALS 397
            |.||||..||:.||:|.|||.:.|.||.....:..|:      ||..|.|             ||
  Rat   327 PTTHTMPTWVKAVFLNLLPRVMFMTRPTSGEGDTPKT------RTFYGAE-------------LS 372

  Fly   398 RMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSS 462
            .::...:..|:|                 |..|               ||..
  Rat   373 NLNCFSRADSKS-----------------CKEG---------------YPCQ------------- 392

  Fly   463 LIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQ 527
                                                         :|..||       .||...:
  Rat   393 ---------------------------------------------DGTCGY-------CHHRRVK 405

  Fly   528 QSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGM 592
            .|:.|||.             |.:|           |:.:|::|.:.|.|:
  Rat   406 ISNFSANL-------------TRSS-----------SSESVDAVLSLSALS-------------- 432

  Fly   593 GAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAA 657
                                                                             
  Rat   433 ----------------------------------------------------------------- 432

  Fly   658 AAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTR 722
                                                           ||:.:|:..|.|||:..:
  Rat   433 -----------------------------------------------PEIKEAIQSVKYIAENMK 450

  Fly   723 KEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQ 763
            .:..:..:::||||||||:||:|||:|.:..::||||:.||
  Rat   451 AQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQ 491

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	108/219 (49%)
		Cys-loop	150..164	CDD:349832	10/13 (77%)
		LGIC_TM_nAChR	242..>331	CDD:349866	62/88 (70%)
		TM1 helix	242..266	CDD:349866	19/23 (83%)
		TM2 helix	274..295	CDD:349866	12/20 (60%)
		TM3 helix	306..328	CDD:349866	12/21 (57%)
		Neur_chan_memb	<683..760	CDD:460753	24/76 (32%)
Chrna3	NP_434692.2	LIC	24..490	CDD:455710	242/742 (33%)
		LGIC_ECD_nAChR_A3	29..235	CDD:349817	108/216 (50%)
		Cys-loop	153..167	CDD:349817	10/13 (77%)