Protein Alignment nAChRalpha3 and acr-16

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_505207.1
• Gene: acr-16 / 179235
• WormBaseID: WBGene00000055
• Length: 498
• Species: Caenorhabditis elegans
• Alignment Length: 742
• Identity: 209/742 - (28%)
• Similarity: 306/742 - (41%)
• Gaps: 270/742 - (36%)

  Fly    27 KRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWEP 91
            :|||:||:.|||.|.|||.|.::.:||.:|:.|.|:|||:.|||::..|.|::.:|.||.|.|:.
 Worm    24 RRLYEDLMRNYNNLERPVANHSEPVTVHLKVALQQIIDVDEKNQVVYVNAWLDYTWNDYNLVWDK 88

  Fly    92 KEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEY 156
            .|||.:..:..|:..||:||::|||:.|.||:.|..|...:..||.|.|.||.|:|.||:||:::
 Worm    89 AEYGNITDVRFPAGKIWKPDVLLYNSVDTNFDSTYQTNMIVYSTGLVHWVPPGIFKISCKIDIQW 153

  Fly   157 FPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRNEKFY 221
            ||||||.|..|||||||||:::||:   ...|      |.|:||:.::.||.:......||||||
 Worm   154 FPFDEQKCFFKFGSWTYDGYKLDLQ---PATG------GFDISEYISNGEWALPLTTVERNEKFY 209

  Fly   222 TCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLSLTVF 286
            .||.|||.|:.|.:.|||:||:|..|||:||:..:.:|:|.|.||.|:|||::|.|::|||:..|
 Worm   210 DCCPEPYPDVHFYLHMRRRTLYYGFNLIMPCILTTLMTLLGFTLPPDAGEKITLQITVLLSICFF 274

  Fly   287 FLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFINQLP 351
            ..:::|:.||||..|||||.|....||:.|.|...||.|||:|:|:|:||.|.||.|.:.:..:|
 Worm   275 LSIVSEMSPPTSEAVPLLGIFFTCCMIVVTASTVFTVYVLNLHYRTPETHDMGPWTRNLLLYWIP 339

  Fly   352 RFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRSTSPHLQH 416
            ..|.|:||.:.::..                   .:|.|                 .||.|:...
 Worm   340 WILRMKRPGHNLTYA-------------------SLPSL-----------------FSTKPNRHS 368

  Fly   417 RVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYH-NQYQQTGGSLL 480
            .....|:.|                            |:.:.:.::..||... |||..|
 Worm   369 ESLIRNIKD----------------------------NEHSLSRANSFDADCRLNQYIMT----- 400

  Fly   481 DHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQHAHQA 545
                             |:..||
 Worm   401 -----------------QSVSNG------------------------------------------ 406

  Fly   546 HASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQSSELLHHQQLH 610
                 .:.||.....:|.|..|...|:..:||                         |:.|:..|
 Worm   407 -----LTSLGSIPSTMISSNGTTTDVSQQATL-------------------------LILHRIYH 441

  Fly   611 HQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPPAGV 675
            ..::
 Worm   442 ELKI------------------------------------------------------------- 445

  Fly   676 CDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKEDWKYVAMV 740
                                                  ||....:..|||::.   .:||:.|||
 Worm   446 --------------------------------------VTKRMIEGDKEEQAC---NNWKFAAMV 469

  Fly   741 LDRLFLWIFTIAVVVGTAGIILQAPTL 767
            :|||.|::|||.::|.|.||...||.|
 Worm   470 VDRLCLYVFTIFIIVSTIGIFWSAPYL 496

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	101/215 (47%)
		Cys-loop	150..164	CDD:349832	9/13 (69%)
		LGIC_TM_nAChR	242..>331	CDD:349866	43/88 (49%)
		TM1 helix	242..266	CDD:349866	10/23 (43%)
		TM2 helix	274..295	CDD:349866	7/20 (35%)
		TM3 helix	306..328	CDD:349866	9/21 (43%)
		Neur_chan_memb	<683..760	CDD:460753	19/76 (25%)
acr-16	NP_505207.1	LIC	6..493	CDD:455710	206/737 (28%)
		LGIC_ECD_nAChR	48..228	CDD:349798	86/188 (46%)
		Cys-loop	147..161	CDD:349798	9/13 (69%)