Protein Alignment nAChRalpha3 and lev-1

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_001255705.1
• Gene: lev-1 / 178269
• WormBaseID: WBGene00002974
• Length: 507
• Species: Caenorhabditis elegans
• Alignment Length: 768
• Identity: 214/768 - (27%)
• Similarity: 312/768 - (40%)
• Gaps: 314/768 - (40%)

  Fly    10 MILVLSFISSSTANP-DAK-RLYDDLLSNYNKLVRPVVNVTD-ALTVRIKLKLSQLIDVNLKNQI 71
            :.|.:|..:.||... ||: ||..||...||.||:||.|.:: .:.|:|.::|..||:|:.|.|:
 Worm    20 VFLAVSLRNHSTCEDIDAEDRLMVDLFRGYNSLVQPVRNRSELPMIVKIGMQLVLLINVDEKEQV 84

  Fly    72 MTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTG 136
            |.||:|:...|.|::|||:|::|..:..:.|..:.:|.|||||:||||||:||:......:..||
 Worm    85 MHTNVWLTMKWDDFQLKWDPRDYANITQIRVAPEKVWLPDIVLFNNADGNYEVSFMCNVLILSTG 149

  Fly   137 RVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEF 201
            .|.|.|||||||||.||||:||||:|.|.:.||||||:..::.|   |.|....     ||.||:
 Worm   150 TVLWVPPAIYKSSCIIDVEFFPFDDQLCSLTFGSWTYNRDEIKL---DFLTSDR-----VDFSEY 206

  Fly   202 YTSVEWDILEVPAV----RNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILV 262
            .||..||:::.|||    |:.            |.|.|.:|||||||||.||:|.:.::||.:.|
 Worm   207 STSSIWDMMDGPAVLTSDRSR------------IEFQIRIRRKTLFYTVVLILPTVLMAFLNVTV 259

  Fly   263 FYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLN 327
            ||||:.||||:.|::::|||:.||.||:::|:||||..:||:.|::|.|.:|:..:|.||.::.|
 Worm   260 FYLPTASGEKMGLTMNVLLSIVVFLLLVSKILPPTSSSIPLVAKYLLLTFVLNIITIMVTTIICN 324

  Fly   328 IHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPP 392
            |:||||.||.:.||||.||::.||..:.|:||
 Worm   325 IYFRSPITHRLPPWVRKVFLDILPLLMCMQRP--------------------------------- 356

  Fly   393 PVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQT 457
                    |......||
 Worm   357 --------HRKNVIQRS------------------------------------------------ 365

  Fly   458 STTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGH 522
                       |.:..:||.|:.::|:                                 :||.|
 Worm   366 -----------HRRLLETGPSVEENPM---------------------------------RSGEH 386

  Fly   523 HLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQ 587
            |             ||.:|.|.                                 .|||....|
 Worm   387 H-------------PLCRHTHN---------------------------------QDSCRRVRI- 404

  Fly   588 IHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSA 652
                       ||.||  ..:|
 Worm   405 -----------QSDEL--DDEL------------------------------------------- 413

  Fly   653 AAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYI 717
                                                               .||..:|:|.:.:|
 Worm   414 ---------------------------------------------------SPEAQRAIDAIEFI 427

  Fly   718 ADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDT 770
            .:..|.||.:.:.::|||::|.|:||..|:.|..|.|.||.|||..||::::|
 Worm   428 TENRRDEEITKQFRDDWKFIASVVDRFLLYGFFGATVGGTIGIIFTAPSVFET 480

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	98/223 (44%)
		Cys-loop	150..164	CDD:349832	10/13 (77%)
		LGIC_TM_nAChR	242..>331	CDD:349866	43/88 (49%)
		TM1 helix	242..266	CDD:349866	13/23 (57%)
		TM2 helix	274..295	CDD:349866	9/20 (45%)
		TM3 helix	306..328	CDD:349866	7/21 (33%)
		Neur_chan_memb	<683..760	CDD:460753	21/76 (28%)
lev-1	NP_001255705.1	LIC	15..474	CDD:455710	211/760 (28%)
		LGIC_ECD_nAChR_proto_beta-like	37..238	CDD:349833	95/220 (43%)
		Cys-loop	163..177	CDD:349833	10/13 (77%)