Protein Alignment nAChRalpha3 and unc-38

DIOPT Version :10

• Sequence 1: NP_525079.3
• Gene: nAChRalpha3 / 31767
• FlyBaseID: FBgn0015519
• Length: 795
• Species: Drosophila melanogaster
• Sequence 2: NP_491472.1
• Gene: unc-38 / 172105
• WormBaseID: WBGene00006774
• Length: 511
• Species: Caenorhabditis elegans
• Alignment Length: 794
• Identity: 264/794 - (33%)
• Similarity: 363/794 - (45%)
• Gaps: 311/794 - (39%)

  Fly    10 MILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTT 74
            ::..:|||..:..|.|||||||||:.|||:..||..:....||:::||:|||:|||:..:||||.
 Worm    11 LLFCISFIKLTEGNEDAKRLYDDLMVNYNRHRRPSTSPNKPLTIKLKLRLSQIIDVHEIDQIMTC 75

  Fly    75 NLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVE 139
            ::|::|:|.|.||.|:|..||||.:|:||.:.||.||||||||||.|:.:|::|||||:|||.|.
 Worm    76 SVWLKQTWIDRKLSWDPVNYGGVNVLYVPYEMIWVPDIVLYNNADSNYNITISTKATLHYTGEVT 140

  Fly   140 WRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDG--FQVDL--------RHIDE---LNGTNV 191
            |.||||:||.|:|||.:||||||.|.:||||||:..  ..|:|        ..|||   ::...|
 Worm   141 WEPPAIFKSMCQIDVRWFPFDEQQCHLKFGSWTFSENLLSVELNEPSLRYEEEIDEKGIIDNVTV 205

  Fly   192 VEVGVDLSEFYTSVEWDILEVPAVRNEKFY-TCCDE-PYLDITFNITMRRKTLFYTVNLIIPCMG 254
            .|.|:|||::|.||||||:...|.|..|.| :||.: .|:|:|:.:.:|||.|||||||:.||:|
 Worm   206 AEDGIDLSDYYPSVEWDIMSRVAKRRAKNYPSCCPQSAYIDVTYYLQLRRKPLFYTVNLVFPCVG 270

  Fly   255 ISFLTILVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSI 319
            |||||||||||||||||||:|.||||::||:|||||.||||.||:.:||:||::||||::.|.|:
 Worm   271 ISFLTILVFYLPSDSGEKVTLCISILVALTIFFLLLTEIIPATSITLPLIGKYLLFTMVMVTLSV 335

  Fly   320 CVTVLVLNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELR 384
            .|||:.||:|||:|.||.|..||:.||:..||:.|.||||:....|                :..
 Worm   336 VVTVISLNLHFRTPTTHLMPNWVKKVFLKWLPKLLFMRRPIDDYEE----------------KFD 384

  Fly   385 DQIPPLPPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPT 449
            |:..|....:|||
 Worm   385 DKKKPKDGKIALS---------------------------------------------------- 397

  Fly   450 MSTANQQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYG 514
                                                                             
 Worm   398 ----------------------------------------------------------------- 397

  Fly   515 GHGQSGGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLAD 579
                                     .|||:      .|::|    |.|::.|..:::
 Worm   398 -------------------------VHAHR------VSNVG----NNIRNATIDDTI-------- 419

  Fly   580 SCCSGTIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAA 644
                                            |:|::.
 Worm   420 --------------------------------QKMYYS--------------------------- 425

  Fly   645 AGGPSTSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHK 709
                                                                        |.:.|
 Worm   426 ------------------------------------------------------------PPVVK 430

  Fly   710 AMDGVTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMPI 774
            |.:.:.:||:..:|::...::.|||||||||||||||.||:||..|||..|:|:||||||||.||
 Worm   431 AFENICFIAELLKKKDRDDKIDEDWKYVAMVLDRLFLLIFSIACFVGTVIILLRAPTLYDTRQPI 495

  Fly   775 DIKLSEIASTTAKP 788
            |::... |:.:|.|
 Worm   496 DLQYRP-ANLSANP 508

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha3	NP_525079.3	LGIC_ECD_nAChR_proto_alpha-like	25..243	CDD:349832	120/232 (52%)
		Cys-loop	150..164	CDD:349832	10/13 (77%)
		LGIC_TM_nAChR	242..>331	CDD:349866	63/88 (72%)
		TM1 helix	242..266	CDD:349866	20/23 (87%)
		TM2 helix	274..295	CDD:349866	14/20 (70%)
		TM3 helix	306..328	CDD:349866	11/21 (52%)
		Neur_chan_memb	<683..760	CDD:460753	28/76 (37%)
unc-38	NP_491472.1	LGIC_ECD_nAChR_proto_alpha-like	26..259	CDD:349832	120/232 (52%)
		Cys-loop	151..165	CDD:349832	10/13 (77%)
		Neur_chan_memb	264..481	CDD:460753	118/511 (23%)